Strain Detail Sheet

Strain Name:
B6J.129X1-Ier3tm1Mxw/Mmjax
Stock Number:
068229-JAX
Citation ID:
RRID:MMRRC_068229-JAX
Other Names:
IEX-1 knockout mice

Strain Information

Gene Details

Ier3tm1Mxw
Name: immediate early response 3; targeted mutation 1, Mei X Wu
Synonyms: IEX-1 KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
Ier3
Name: immediate early response 3
Synonyms: cI-3, IEX-1, cAMP inducible gene 3, gly96
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Knockout
NCBI: 15937
HGNC: HGNC:5392
Homologene: 2894
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Additional Resources
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Genetic Alterations

A 1.5-kb region including part of exon 1, all of intron 1, and most of exon 2 was replaced with a lacZ, pgk-neo cassette via homologous recombination.

Genotype Determination
ES Cell Line
Unspecified derived from 129X1/SvJ

Strain Description

Phenotype
None/Normal/Wild-type
MeSH Terms
  • Animals
  • Aorta/drug effects
  • Aorta/metabolism
  • Aorta/physiology
  • Blood Pressure/physiology
  • Blotting, Western
  • Cells, Cultured
  • Cyclic AMP/metabolism
  • Female
  • Free Radical Scavengers/pharmacology
  • GTP-Binding Protein alpha Subunit, Gi2/genetics
  • GTP-Binding Protein alpha Subunit, Gi2/metabolism
  • Immediate-Early Proteins/genetics
  • Immediate-Early Proteins/metabolism
  • In Vitro Techniques
  • Male
  • Metalloporphyrins/pharmacology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mice, Knockout
  • Mitochondria/metabolism
  • Muscle, Smooth, Vascular/cytology
  • Muscle, Smooth, Vascular/metabolism
  • Myocytes, Smooth Muscle/cytology
  • Myocytes, Smooth Muscle/drug effects
  • Myocytes, Smooth Muscle/metabolism
  • Pertussis Toxin/pharmacology
  • Rotenone/pharmacology
  • Signal Transduction/physiology
  • Superoxides/antagonists & inhibitors
  • Superoxides/metabolism
  • Vasodilation/drug effects
Strain Development
Unspecified ES cells derived from 129X1/SvJ. Chimeras were bred to C57BL/6J, followed by backcrossing to C57BL/6J to minimally N7 and sib-mating (F?, generation data unavailable).
Suggested Control Mice
Wild-type sibling

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Apoptosis
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Hematology
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Research Tools

Strain Origin

Donor
Mei X. Wu, Ph.D., Harvard Medical School.
Primary Reference

Shahid M, Shen L, Seldin DC, Lu B, Ustyugova IV, Chen X, Zapol WM, Wu MX. Impaired 3',5'-cyclic adenosine monophosphate-mediated signaling in immediate early responsive gene X-1-deficient vascular smooth muscle cells. Hypertension. 2010 Oct;56(4):705-12. doi: 10.1161/HYPERTENSIONAHA.110.154880. Epub 2010 Aug 16. (Medline PMID: 20713914)

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Agouti
Eye
Black

Breeding

MMRRC Breeding System
Backcross and sib-mating
Generation
N7 (C57BL/6J), F?
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months 7 to 9 months
Bred to Homozygosity
Yes
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.