Strain Name:
B6J.Cg-Baiap2l2em1Pgg/Mmucd
Stock Number:
069633-UCD
Citation ID:
RRID:MMRRC_069633-UCD
Other Names:
Baiap2L2

Strain Information

Baiap2l2em1Pgg
Name: BAI1-associated protein 2-like 2; endonuclease-mediated mutation 1, Peter G Gillespie
Synonyms: Baiap2l2delta16
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: CRISPR
Baiap2l2
Name: BAI1-associated protein 2-like 2
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: CRISPR
NCBI: 207495
Homologene: 11811
Genetic Alterations
The Baiap2L2 locus was targeted for CRISPR-mediated knockout. Founders were screened, which identified an out-of-frame mutation in the coding region of exon 4 (c.247_262delCGGCACTTGAACTCAG) that is predicted to result in a premature termination codon (p.Arg83ThrfsTer67).
HVGS Nomenclature
  • Genbank RefSeq - mRNA: NM_177580.3
  • Genbank RefSeq, protein: NP_808248.1
  • Variant, nucleic acid level: c.247_262delCGGCACTTGAACTCAG
  • Variant, amino acid level, predicted: p.Arg83ThrfsTer67
  • Check this variant: LUMC Mutalyzer
ES Cell Line
Not applicable
Phenotype
No behavioral phenotype is displayed in homozygous mutant animals. Homozygous mutant animals have elevated ABR threshholds at all tested frequencies.
Strain Development
The Baiap2l2 locus was targeted for CRISPR-mediated knockout using guide RNAs (gRNAs) designed for exons 4 and 10. A mixture containing gRNA and Cas9 mRNA was injected into F1 hybrid C57BL/6J xC57BL/6NJ zygotes and implanted into pseudopregnant females. The line was backcrossed onto the C57BL/6J background.
Suggested Control Mice
Heterozygous and wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Neurobiology
  • Obesity
  • Sensorineural
Donor
Peter Barr-Gillespie, Ph.D., Oregon Health & Science University.
Primary Reference

Halford J, Bateschell M, Barr-Gillespie PG. Ca2+ entry through mechanotransduction channels localizes BAIAP2L2 to stereocilia tips. Mol Biol Cell. 2022 Jan 19:mbcE21100491. doi: 10.1091/mbc.E21-10-0491. Epub ahead of print. (Medline PMID: 35044843)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross and random intra-strain mating
Generation
N12 (C57BL/6J), F5
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 10 to 12 months
Average litter size
4 to 6
Recommended wean age
4 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069633-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
069633-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.