Strain Name    : B6.Cg-Siglec15tm1.2Cfg/Mmucd

Stock Number : 032722-UCD

Other Names   : Siglec15-KO

Gene Information

(provided by MGI)
Allele Symbol: Siglec15tm1.2Cfg
Name: targeted mutation 1.2, Consortium for Functional Glycomics
Chromosome: 18
Alteration at locus: Knockout
Gene Symbol: Siglec15
Name: sialic acid binding Ig-like lectin 15
Chromosome: 18
Alteration at locus: Knockout
Genetic Alterations:
The Siglec15 gene consists of 6 exons. A loxP-flanked selection vector containing neomycin and thymidine kinase was used in the construction of this mutant. The positive and negative selection vector was inserted downstream of exon 5 of the targeted gene and a third loxP site was inserted upstream of exon 2 via homologous recombination. Diagram of construct design

Genotype Determination:

ES Cell Line: Bruce4 derived from B6.Cg-Thy1

Phenotype
Phenotype data can be accessed at the Consortium for Functional Glycomics Gateway.

Mammalian Phenotype Terms:

Strain of Origin: B6.Cg

Strain genetic background: B6.Cg

Strain Development: The targeting construct was electroporated into Bruce4 embryonic stem (ES) cells. Correctly targeted ES cells containing the floxed allele were injected into BALB/c blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and further crossed to ubiquitous Cre-recombinase mice,  C57BL/6N-Hprttm1(CMV-cre)Brd/Mmucd, (MMRRC:034412) to remove the cassette and exons 2 through 5.

Suggested Control Mice: Wild-type littermates

Donor: Jim Paulson, Ph.D., The Scripps Research Institute.

Primary Reference:

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: Black

Other:

MMRRC Breeding System: Backcross or Sib-mating

Breeding Scheme(s):

  • C57BL/6J female x Heterozygous male or reciprocal mating
  • Wild-type female x Herterozygous male siblings or reciprocal mating
  • Heterozygous female x Heterozygous male siblings
  • Homozygous female x Homozygous male siblings

Generation:

Overall Breeding Performance: Good

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Average litter size: 6-8

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
032722-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
032722-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.