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Strain Name:
C57BL/6J-MtgxR0022Btlr/Mmmh
Stock Number:
038317-MU
Citation ID:
RRID:MMRRC_038317-MU
Other Names:
R0022 (G1), C57BL/6J-MtgxR0022Btlr
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Elavl3
Name: ELAV like RNA binding protein 3
Synonyms: mHuC, Huc, 2600009P04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15571
VEGA: 9
HGNC: HGNC:3314
Homologene: 31035
Top1
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21969
Homologene: 2467
Mcm7
Name: minichromosome maintenance complex component 7
Synonyms: mCDC47, Mcmd7
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17220
HGNC: HGNC:6950
Homologene: 4323
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 34,866,239 bp
  • C to T, chromosome 1 at 37,628,245 bp
  • G to A, chromosome 1 at 45,383,683 bp
  • T to A, chromosome 1 at 52,140,630 bp
  • T to C, chromosome 1 at 65,103,223 bp
  • T to A, chromosome 1 at 105,691,902 bp
  • A to T, chromosome 1 at 153,760,775 bp
  • A to G, chromosome 1 at 171,573,762 bp
  • A to G, chromosome 1 at 173,683,046 bp
  • A to G, chromosome 2 at 4,899,560 bp
  • A to T, chromosome 2 at 40,998,038 bp
  • A to G, chromosome 2 at 111,257,649 bp
  • A to G, chromosome 2 at 112,640,666 bp
  • A to T, chromosome 2 at 120,961,173 bp
  • A to G, chromosome 2 at 121,368,393 bp
  • A to T, chromosome 2 at 154,230,348 bp
  • A to C, chromosome 2 at 160,702,799 bp
  • A to G, chromosome 2 at 180,619,756 bp
  • G to A, chromosome 3 at 79,087,900 bp
  • G to T, chromosome 3 at 103,803,148 bp
  • A to T, chromosome 3 at 107,753,862 bp
  • A to T, chromosome 3 at 116,793,836 bp
  • G to A, chromosome 3 at 121,988,937 bp
  • G to A, chromosome 4 at 94,837,272 bp
  • T to A, chromosome 4 at 118,435,489 bp
  • A to G, chromosome 5 at 75,622,997 bp
  • C to A, chromosome 5 at 121,417,976 bp
  • T to C, chromosome 5 at 138,164,719 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • T to C, chromosome 6 at 41,614,569 bp
  • T to C, chromosome 6 at 52,217,383 bp
  • C to A, chromosome 6 at 54,985,902 bp
  • A to G, chromosome 6 at 67,298,919 bp
  • T to C, chromosome 6 at 122,900,395 bp
  • T to A, chromosome 6 at 137,443,594 bp
  • T to A, chromosome 6 at 145,429,990 bp
  • A to G, chromosome 7 at 29,792,435 bp
  • A to T, chromosome 7 at 97,982,875 bp
  • G to A, chromosome 7 at 113,325,781 bp
  • A to G, chromosome 8 at 70,770,901 bp
  • C to T, chromosome 8 at 109,525,407 bp
  • C to A, chromosome 9 at 22,036,871 bp
  • A to G, chromosome 9 at 24,696,124 bp
  • T to A, chromosome 9 at 105,396,624 bp
  • C to T, chromosome 10 at 12,709,956 bp
  • G to T, chromosome 10 at 23,920,727 bp
  • A to G, chromosome 10 at 121,671,770 bp
  • T to A, chromosome 11 at 60,780,359 bp
  • T to G, chromosome 11 at 71,123,381 bp
  • T to G, chromosome 11 at 71,161,929 bp
  • T to C, chromosome 11 at 77,843,233 bp
  • A to T, chromosome 11 at 116,297,582 bp
  • A to T, chromosome 11 at 116,349,450 bp
  • G to A, chromosome 12 at 33,082,264 bp
  • A to T, chromosome 14 at 52,232,855 bp
  • A to G, chromosome 14 at 56,166,925 bp
  • G to A, chromosome 15 at 12,371,605 bp
  • A to G, chromosome 15 at 89,163,276 bp
  • A to T, chromosome 16 at 4,085,228 bp
  • C to T, chromosome 16 at 4,633,304 bp
  • A to T, chromosome 17 at 9,669,216 bp
  • A to G, chromosome 17 at 12,890,119 bp
  • T to C, chromosome 17 at 24,594,819 bp
  • T to A, chromosome 17 at 75,364,360 bp
  • C to G, chromosome 18 at 44,519,516 bp
  • T to C, chromosome 18 at 63,777,634 bp
  • T to G, chromosome 18 at 68,338,782 bp
  • T to G, chromosome 19 at 4,194,581 bp
  • C to G, chromosome X at 150,647,512 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0022 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038317-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.