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Strain Name:
C57BL/6J-MtgxR0285Btlr/Mmmh
Stock Number:
038506-MU
Citation ID:
RRID:MMRRC_038506-MU
Other Names:
R0285 (G1), C57BL/6J-MtgxR0285Btlr
Major Collection:

Strain Information

Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Gpd2
Name: glycerol phosphate dehydrogenase 2, mitochondrial
Synonyms: Gdm1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14571
HGNC: HGNC:4456
Homologene: 352
Card11
Name: caspase recruitment domain family, member 11
Synonyms: CARMA1, BIMP3, 2410011D02Rik, 0610008L17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108723
Homologene: 13024
Washc2
Name: WASH complex subunit 2
Synonyms: C530005J20Rik, D6Wsu116e, Fam21
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28006
Homologene: 41686
Diaph3
Name: diaphanous related formin 3
Synonyms: p134MDia2, mDia2, 4930417P13Rik, Drf3, Diap3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Dop1a
Name: DOP1 leucine zipper like protein A
Synonyms: B130005I07Rik, D9Ertd809e, Dopey1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320615
Homologene: 26645
Tmem87a
Name: transmembrane protein 87A
Synonyms: A930025J12Rik, Elkin1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 211499
Homologene: 9165
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 156,845,215 bp
  • T to G, chromosome 2 at 26,460,861 bp
  • T to G, chromosome 2 at 28,550,569 bp
  • C to A, chromosome 2 at 32,823,542 bp
  • A to T, chromosome 2 at 57,338,955 bp
  • T to C, chromosome 2 at 60,318,319 bp
  • A to G, chromosome 2 at 87,950,131 bp
  • A to G, chromosome 2 at 120,394,424 bp
  • G to A, chromosome 2 at 153,483,853 bp
  • TGC to TGCGC, chromosome 2 at 155,408,291 bp
  • T to C, chromosome 2 at 155,415,701 bp
  • T to C, chromosome 3 at 30,684,948 bp
  • G to A, chromosome 3 at 79,839,273 bp
  • G to T, chromosome 3 at 88,096,990 bp
  • A to G, chromosome 4 at 21,798,603 bp
  • G to A, chromosome 4 at 42,850,236 bp
  • G to A, chromosome 4 at 43,854,398 bp
  • A to C, chromosome 4 at 139,440,801 bp
  • C to T, chromosome 5 at 30,379,533 bp
  • T to A, chromosome 5 at 65,806,559 bp
  • A to G, chromosome 5 at 86,271,430 bp
  • T to C, chromosome 5 at 101,797,038 bp
  • C to A, chromosome 5 at 116,467,789 bp
  • G to A, chromosome 5 at 140,887,101 bp
  • T to C, chromosome 6 at 37,984,522 bp
  • C to A, chromosome 6 at 57,209,994 bp
  • G to A, chromosome 6 at 91,498,064 bp
  • T to A, chromosome 6 at 116,221,839 bp
  • A to G, chromosome 7 at 5,110,378 bp
  • T to A, chromosome 7 at 13,628,760 bp
  • C to A, chromosome 7 at 27,673,900 bp
  • A to G, chromosome 7 at 30,645,840 bp
  • A to G, chromosome 7 at 86,742,165 bp
  • A to T, chromosome 7 at 104,189,324 bp
  • A to G, chromosome 7 at 108,346,499 bp
  • T to C, chromosome 7 at 140,129,140 bp
  • A to C, chromosome 8 at 63,928,789 bp
  • A to T, chromosome 8 at 82,034,516 bp
  • G to C, chromosome 8 at 110,893,717 bp
  • T to A, chromosome 9 at 38,930,774 bp
  • A to T, chromosome 9 at 86,512,639 bp
  • T to C, chromosome 9 at 108,378,564 bp
  • C to A, chromosome 9 at 116,030,738 bp
  • C to T, chromosome 9 at 119,070,827 bp
  • T to C, chromosome 10 at 75,925,668 bp
  • T to C, chromosome 10 at 76,062,283 bp
  • T to A, chromosome 10 at 79,396,557 bp
  • G to A, chromosome 10 at 115,290,863 bp
  • T to A, chromosome 10 at 125,294,631 bp
  • T to A, chromosome 10 at 128,512,218 bp
  • A to T, chromosome 11 at 53,959,196 bp
  • G to A, chromosome 11 at 82,062,258 bp
  • A to G, chromosome 11 at 102,672,191 bp
  • A to T, chromosome 11 at 120,640,316 bp
  • C to A, chromosome 12 at 31,326,645 bp
  • A to G, chromosome 12 at 56,858,025 bp
  • T to C, chromosome 13 at 11,716,977 bp
  • A to G, chromosome 13 at 38,172,794 bp
  • A to T, chromosome 13 at 49,263,948 bp
  • A to G, chromosome 13 at 75,877,509 bp
  • G to A, chromosome 14 at 55,601,389 bp
  • G to A, chromosome 14 at 87,115,024 bp
  • T to C, chromosome 15 at 60,822,967 bp
  • T to A, chromosome 15 at 78,452,868 bp
  • C to T, chromosome 15 at 89,336,274 bp
  • A to G, chromosome 15 at 97,798,222 bp
  • A to G, chromosome 17 at 17,374,680 bp
  • A to G, chromosome 17 at 20,345,611 bp
  • A to G, chromosome 17 at 26,939,449 bp
  • C to T, chromosome 17 at 34,650,396 bp
  • A to G, chromosome 17 at 78,808,453 bp
  • A to G, chromosome 18 at 21,129,612 bp
  • G to A, chromosome 18 at 57,533,865 bp
  • C to A, chromosome 18 at 84,618,238 bp
  • A to G, chromosome 19 at 3,988,193 bp
  • A to G, chromosome 19 at 11,871,138 bp
  • A to T, chromosome 19 at 12,935,172 bp
  • G to A, chromosome 19 at 23,979,385 bp
  • A to T, chromosome 19 at 42,576,129 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0285 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038506-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.