Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR0336Btlr/Mmmh
Stock Number:
038545-MU
Citation ID:
RRID:MMRRC_038545-MU
Other Names:
R0336 (G1), C57BL/6J-MtgxR0336Btlr
Major Collection:

Strain Information

Fga
Name: fibrinogen alpha chain
Synonyms: Fib, ENSMUSG00000059807
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14161
HGNC: HGNC:3661
Homologene: 428
Rfx3
Name: regulatory factor X, 3 (influences HLA class II expression)
Synonyms: MRFX3, C230093O12Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19726
HGNC: HGNC:9984
Homologene: 7917
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Lrig3
Name: leucine-rich repeats and immunoglobulin-like domains 3
Synonyms: 9030421L11Rik, 9130004I02Rik, 9430095K15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320398
VEGA: 10
Homologene: 62452
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Rnf38
Name: ring finger protein 38
Synonyms: Oip1, 1700065B19Rik, 2610202O07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 73469
Homologene: 32550
Tlr6
Name: toll-like receptor 6
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21899
Homologene: 21223
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 95,653,558 bp
  • T to C, chromosome 2 at 88,729,154 bp
  • A to G, chromosome 2 at 129,015,412 bp
  • C to A, chromosome 2 at 163,832,322 bp
  • T to C, chromosome 3 at 83,030,857 bp
  • T to A, chromosome 3 at 96,559,979 bp
  • A to G, chromosome 3 at 102,735,027 bp
  • A to T, chromosome 4 at 44,152,350 bp
  • T to C, chromosome 4 at 88,827,941 bp
  • G to A, chromosome 4 at 120,103,847 bp
  • A to C, chromosome 4 at 147,613,135 bp
  • G to T, chromosome 5 at 33,655,602 bp
  • G to T, chromosome 5 at 64,953,946 bp
  • A to G, chromosome 5 at 76,601,502 bp
  • C to A, chromosome 5 at 88,663,153 bp
  • A to G, chromosome 5 at 110,447,951 bp
  • A to T, chromosome 5 at 135,428,613 bp
  • A to G, chromosome 5 at 149,746,592 bp
  • G to A, chromosome 6 at 88,849,595 bp
  • G to A, chromosome 7 at 5,611,067 bp
  • T to C, chromosome 7 at 12,305,321 bp
  • T to C, chromosome 7 at 16,435,178 bp
  • T to A, chromosome 7 at 21,098,462 bp
  • T to A, chromosome 7 at 24,921,957 bp
  • T to C, chromosome 7 at 44,505,616 bp
  • T to A, chromosome 7 at 45,286,468 bp
  • A to T, chromosome 7 at 67,662,483 bp
  • A to C, chromosome 7 at 87,002,079 bp
  • T to C, chromosome 7 at 109,331,474 bp
  • C to A, chromosome 8 at 18,940,636 bp
  • G to T, chromosome 8 at 19,052,969 bp
  • T to C, chromosome 8 at 71,890,605 bp
  • A to T, chromosome 9 at 79,702,345 bp
  • A to G, chromosome 9 at 90,075,738 bp
  • G to T, chromosome 9 at 114,330,285 bp
  • C to A, chromosome 10 at 39,526,901 bp
  • T to A, chromosome 10 at 51,481,293 bp
  • A to G, chromosome 10 at 77,058,736 bp
  • C to T, chromosome 10 at 125,966,705 bp
  • A to G, chromosome 11 at 9,298,481 bp
  • C to T, chromosome 11 at 69,736,893 bp
  • A to G, chromosome 11 at 101,523,993 bp
  • A to C, chromosome 11 at 113,662,250 bp
  • A to G, chromosome 11 at 115,787,385 bp
  • A to G, chromosome 12 at 51,661,608 bp
  • T to C, chromosome 12 at 88,178,191 bp
  • T to C, chromosome 12 at 111,733,658 bp
  • A to G, chromosome 13 at 70,791,794 bp
  • T to A, chromosome 14 at 20,483,186 bp
  • C to T, chromosome 14 at 98,168,748 bp
  • G to A, chromosome 15 at 6,776,753 bp
  • T to G, chromosome 15 at 35,455,133 bp
  • A to G, chromosome 15 at 74,587,149 bp
  • C to T, chromosome 15 at 83,836,282 bp
  • A to T, chromosome 15 at 100,699,206 bp
  • A to G, chromosome 16 at 3,866,379 bp
  • A to C, chromosome 16 at 4,044,626 bp
  • A to G, chromosome 16 at 63,566,648 bp
  • C to T, chromosome 17 at 7,765,107 bp
  • C to A, chromosome 17 at 22,339,028 bp
  • C to T, chromosome 17 at 73,922,463 bp
  • C to T, chromosome 19 at 6,388,758 bp
  • A to G, chromosome 19 at 27,806,262 bp
  • A to T, chromosome 19 at 29,587,793 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0336 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038545-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.