Strain Name:
C57BL/6J-MtgxR1612Btlr/Mmmh
Stock Number:
039649-MU
Citation ID:
RRID:MMRRC_039649-MU
Other Names:
R1612 (G1), C57BL/6J-MtgxR1612Btlr
Major Collection:

Gene Information

Gene Symbol: Slc6a6 [MGI:98488] (Mus musculus (mouse))
Name: solute carrier family 6 (neurotransmitter transporter, taurine), member 6
Synonyms: Taut
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Wsb1 [MGI:1926139] (Mus musculus (mouse))
Name: WD repeat and SOCS box-containing 1
Synonyms: 1110056B13Rik, 2700038M07Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Cd14 [MGI:88318] (Mus musculus (mouse))
Name: CD14 antigen
Chromosome: 18
Alteration at locus: Chemically Induced
Gene Symbol: Plk3 [MGI:109604] (Mus musculus (mouse))
Name: polo like kinase 3
Synonyms: Cnk
Chromosome: 4
Alteration at locus: Chemically Induced
Gene Symbol: Pigl [MGI:2681271] (Mus musculus (mouse))
Name: phosphatidylinositol glycan anchor biosynthesis, class L
Synonyms: LOC327942
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Sptan1 [MGI:98386] (Mus musculus (mouse))
Name: spectrin alpha, non-erythrocytic 1
Synonyms: 2610027H02Rik, alphaII-spectrin, Spna-2, alpha-fodrin, Spna2
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Eps8 [MGI:104684] (Mus musculus (mouse))
Name: epidermal growth factor receptor pathway substrate 8
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 162,967,885 bp
  • A to T, chromosome 1 at 171,221,902 bp
  • A to T, chromosome 1 at 176,788,457 bp
  • T to C, chromosome 2 at 25,213,854 bp
  • C to G, chromosome 2 at 30,003,336 bp
  • C to T, chromosome 2 at 84,806,373 bp
  • T to C, chromosome 2 at 87,656,087 bp
  • T to C, chromosome 2 at 121,139,120 bp
  • A to T, chromosome 2 at 172,427,078 bp
  • A to G, chromosome 3 at 79,631,144 bp
  • C to T, chromosome 3 at 86,805,639 bp
  • C to T, chromosome 3 at 87,751,853 bp
  • T to C, chromosome 3 at 100,151,199 bp
  • T to C, chromosome 3 at 138,228,881 bp
  • T to A, chromosome 4 at 52,911,501 bp
  • G to A, chromosome 4 at 62,625,935 bp
  • A to G, chromosome 4 at 107,395,068 bp
  • A to G, chromosome 4 at 117,131,807 bp
  • A to T, chromosome 4 at 129,686,922 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • T to C, chromosome 5 at 24,877,028 bp
  • C to A, chromosome 5 at 36,796,362 bp
  • G to T, chromosome 5 at 105,326,596 bp
  • A to G, chromosome 5 at 142,905,595 bp
  • A to G, chromosome 6 at 91,741,027 bp
  • G to T, chromosome 6 at 127,603,929 bp
  • G to A, chromosome 6 at 137,500,618 bp
  • G to T, chromosome 7 at 3,695,396 bp
  • T to A, chromosome 7 at 49,571,211 bp
  • T to C, chromosome 7 at 99,348,931 bp
  • T to A, chromosome 7 at 114,519,556 bp
  • A to G, chromosome 7 at 118,509,911 bp
  • T to G, chromosome 7 at 119,486,975 bp
  • T to A, chromosome 8 at 71,392,379 bp
  • A to G, chromosome 8 at 80,614,861 bp
  • A to T, chromosome 9 at 88,376,905 bp
  • T to C, chromosome 9 at 90,188,697 bp
  • A to G, chromosome 9 at 105,777,549 bp
  • A to G, chromosome 9 at 123,518,154 bp
  • T to G, chromosome 10 at 21,358,835 bp
  • A to G, chromosome 10 at 79,899,245 bp
  • T to A, chromosome 10 at 80,233,055 bp
  • T to A, chromosome 10 at 88,428,482 bp
  • T to C, chromosome 11 at 4,739,198 bp
  • A to T, chromosome 11 at 52,384,070 bp
  • T to A, chromosome 11 at 62,512,994 bp
  • C to T, chromosome 11 at 67,742,207 bp
  • T to A, chromosome 11 at 79,248,585 bp
  • A to C, chromosome 11 at 115,393,406 bp
  • C to A, chromosome 11 at 120,377,088 bp
  • T to A, chromosome 13 at 3,560,051 bp
  • A to T, chromosome 13 at 6,588,589 bp
  • T to C, chromosome 13 at 64,111,510 bp
  • G to T, chromosome 14 at 16,290,867 bp
  • A to G, chromosome 14 at 52,004,081 bp
  • A to T, chromosome 15 at 101,488,211 bp
  • A to T, chromosome 15 at 101,951,844 bp
  • T to C, chromosome 16 at 59,149,624 bp
  • G to A, chromosome 17 at 21,129,915 bp
  • A to G, chromosome 17 at 37,070,669 bp
  • A to G, chromosome 17 at 46,590,251 bp
  • T to C, chromosome 17 at 87,639,938 bp
  • G to A, chromosome 18 at 36,725,665 bp
  • G to T, chromosome 18 at 46,894,703 bp
  • A to T, chromosome 18 at 58,061,752 bp
  • T to A, chromosome 18 at 89,095,660 bp
  • A to G, chromosome 19 at 6,343,341 bp
  • A to G, chromosome 19 at 29,717,845 bp
  • A to G, chromosome 19 at 60,874,434 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1612 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039649-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.