Strain Name:
C57BL/6J-MtgxR1999Btlr/Mmmh
Stock Number:
040009-MU
Citation ID:
RRID:MMRRC_040009-MU
Other Names:
R1999 (G1), C57BL/6J-MtgxR1999Btlr
Major Collection:

Strain Information

Epha7
Name: Eph receptor A7
Synonyms: MDK1, Ebk, Cek11, Ehk3, Hek11, Mdk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13841
HGNC: HGNC:3390
Homologene: 20935
Dnajc7
Name: DnaJ heat shock protein family (Hsp40) member C7
Synonyms: mDj11, Ttc2, mTpr2, 2010003F24Rik, 2010004G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56354
Homologene: 68306
Pola2
Name: polymerase (DNA directed), alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18969
VEGA: 19
Homologene: 48121
Dnmt3b
Name: DNA methyltransferase 3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13436
HGNC: HGNC:2979
Homologene: 56000
Diaph3
Name: diaphanous related formin 3
Synonyms: p134MDia2, mDia2, 4930417P13Rik, Drf3, Diap3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56419
VEGA: 14
Homologene: 84829
Anln
Name: anillin, actin binding protein
Synonyms: 1110037A17Rik, Scraps, 2900037I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68743
VEGA: 9
Homologene: 41281
Nomo1
Name: nodal modulator 1
Synonyms: PM5, Nomo, D7Ertd156e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 211548
Homologene: 13810
Bltp1
Name: bridge-like lipid transfer protein family member 1
Synonyms: Tweek, FSA, 4932438A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229227
Homologene: 52105
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Arhgap32
Name: Rho GTPase activating protein 32
Synonyms: p200RhoGAP, GC-GAP, PX-RICS, 3426406O18Rik, Grit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 330914
Homologene: 8812
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: MAP1C, dynein heavy chain, retrograde transport, Dnec1, Loa, 9930018I23Rik, Dnchc1, Swl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Senp1
Name: SUMO1/sentrin specific peptidase 1
Synonyms: 2310046A20Rik, E330036L07Rik, D15Ertd528e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223870
Homologene: 8731
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Rbbp6
Name: retinoblastoma binding protein 6, ubiquitin ligase
Synonyms: 4933422O15Rik, C030034J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19647
HGNC: HGNC:9889
Homologene: 136812
Otof
Name: otoferlin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 83762
HGNC: HGNC:8515
Homologene: 12892
Rsbn1l
Name: round spermatid basic protein 1-like
Synonyms: 8430412F05Rik, C330002G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 242860
Homologene: 19435
Hoxa3
Name: homeobox A3
Synonyms: Hox-1.5, Mo-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15400
HGNC: HGNC:5104
Homologene: 40725
Slitrk3
Name: SLIT and NTRK-like family, member 3
Synonyms: ST3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 386750
Homologene: 8955
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269700
Homologene: 28297
Htr5a
Name: 5-hydroxytryptamine (serotonin) receptor 5A
Synonyms: Htr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15563
HGNC: HGNC:5300
Homologene: 22461
Sycp2l
Name: synaptonemal complex protein 2-like
Synonyms: LOC218175, EG621792, Gm40956
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 637277
Homologene: 52882
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26875
Homologene: 69111
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 192190
Homologene: 16332
Gpld1
Name: glycosylphosphatidylinositol specific phospholipase D1
Synonyms: 6330541J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14756
HGNC: HGNC:4459
Homologene: 1152
Kif1a
Name: kinesin family member 1A
Synonyms: Kns1, ATSV, N-3 kinesin, LOC381283, C630002N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16560
HGNC: HGNC:888
Homologene: 99729
Slc44a5
Name: solute carrier family 44, member 5
Synonyms: LOC242259
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242259
Homologene: 72094
Stard9
Name: START domain containing 9
Synonyms: N-3 kinesin, Kif16a, 4831403C07Rik, E230025N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668880
Homologene: 130712
Myh1
Name: myosin, heavy polypeptide 1, skeletal muscle, adult
Synonyms: MyHC-IId/x, Myhs-f2, Myhs-f, Myhsf2, A530084A17Rik, MYHC-IIX, myosin heavy chain 2X, IId, IId/x
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17879
HGNC: HGNC:7567
Homologene: 133718
Nek9
Name: NIMA (never in mitosis gene a)-related expressed kinase 9
Synonyms: C130021H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217718
Homologene: 13222
Vmn1r195
Name: vomeronasal 1 receptor 195
Synonyms: V1ri6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 171257
Homologene: 110880
Kcnt1
Name: potassium channel, subfamily T, member 1
Synonyms: Slack, slo2, C030030G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227632
Homologene: 11055
Cd79b
Name: CD79B antigen
Synonyms: B29, Igb, Igbeta, Ig-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15985
HGNC: HGNC:1699
Homologene: 521
Itgb7
Name: integrin beta 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16421
HGNC: HGNC:6162
Homologene: 20247
Acot1
Name: acyl-CoA thioesterase 1
Synonyms: D12Ucla1, CTE-1, ACH2, CTE-I, Cte1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 26897
VEGA: 12
Homologene: 22686
Manea
Name: mannosidase, endo-alpha
Synonyms: 4932703L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242362
Homologene: 11636
Spef2
Name: sperm flagellar 2
Synonyms: C230086A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320277
Homologene: 23371
Krt84
Name: keratin 84
Synonyms: HRb-1, Krt2-3, Krt2-16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16680
VEGA: 15
HGNC: HGNC:6461
Homologene: 22473
Ssc5d
Name: scavenger receptor cysteine rich family, 5 domains
Synonyms: s5d-srcrb, A430110N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269855
Homologene: 77555
Tectb
Name: tectorin beta
Synonyms: [b]-tectorin, Tctnb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 21684
Homologene: 7568
Ces2h
Name: carboxylesterase 2H
Synonyms: Gm5744
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 436059
HGNC: HGNC:1864
Homologene: 128645
Dab2
Name: disabled 2, mitogen-responsive phosphoprotein
Synonyms: D15Wsu122e, 5730435J12Rik, p96, D630005B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13132
HGNC: HGNC:2662
Homologene: 1026
Adprm
Name: ADP-ribose/CDP-alcohol diphosphatase, manganese dependent
Synonyms: 2310004I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66358
Homologene: 10632
Rad21l
Name: RAD21-like (S. pombe)
Synonyms: Gm14160
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668929
Homologene: 82650
Ccdc63
Name: coiled-coil domain containing 63
Synonyms: 4921511C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330188
Homologene: 45136
Apc2
Name: APC regulator of WNT signaling pathway 2
Synonyms: APCL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 23805
Homologene: 4299
Zfp956
Name: zinc finger protein 956
Synonyms: AI894139
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101197
Homologene: 128449
Tmem8b
Name: transmembrane protein 8B
Synonyms: 4930500O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242409
Homologene: 72894
Or8k30
Name: olfactory receptor family 8 subfamily K member 30
Synonyms: GA_x6K02T2Q125-47993761-47994702, MOR189-2, Olfr1076
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258401
Homologene: 17233
Flt4
Name: FMS-like tyrosine kinase 4
Synonyms: VEGFR3, VEGFR-3, Flt-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14257
HGNC: HGNC:3767
Homologene: 7321
Gvin-ps6
Name: GTPase, very large interferon inducible, pseudogene 6
Synonyms: Gm4759
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 209380
Vmn1r74
Name: vomeronasal 1 receptor 74
Synonyms: V1rg5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171240
Homologene: 110796
Ceacam5
Name: CEA cell adhesion molecule 5
Synonyms: 1600029H12Rik, Psg30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73250
Homologene: 115938
Mtmr6
Name: myotubularin related protein 6
Synonyms: 4022440C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219135
VEGA: 14
HGNC: HGNC:7453
Homologene: 55842
Etfb
Name: electron transferring flavoprotein, beta polypeptide
Synonyms: 2810441H06Rik, 0610009I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 110826
HGNC: HGNC:3482
Homologene: 1503
Or3a1b
Name: olfactory receptor family 3 subfamily A member 1B
Synonyms: GA_x6K02T2P1NL-4278037-4278984, MOR255-6, Olfr401
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258701
HGNC: HGNC:8282
Homologene: 73922
Fndc3c1
Name: fibronectin type III domain containing 3C1
Synonyms: LOC333564
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 333564
Homologene: 46440
Kcnq5
Name: potassium voltage-gated channel, subfamily Q, member 5
Synonyms: 9230107O05Rik, D1Mgi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226922
HGNC: HGNC:6299
Homologene: 28270
Or10g6
Name: olfactory receptor family 10 subfamily G member 6
Synonyms: GA_x6K02T2PVTD-33720892-33721824, MOR223-8, Olfr981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258283
VEGA: 9
Homologene: 64849
Gm6797
Name: predicted pseudogene 6797
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 387284
Homologene: 85956
Vmn2r-ps158
Name: vomeronasal 2, receptor, pseudogene 158
Synonyms: Gm9268, Vmn2r126
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668612
Homologene: 104832
Ambp
Name: alpha 1 microglobulin/bikunin precursor
Synonyms: ASPI, HI-30, ulinastatin, Urinary Trypsin Inhibitor, UTI, Itil, Intin4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11699
HGNC: HGNC:453
Homologene: 1234
Prl2c2
Name: prolactin family 2, subfamily c, member 2
Synonyms: MRP-1, PLF-1, Plf, Plf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18811
VEGA: 13
HGNC: HGNC:9445
Homologene: 40763
Mboat2
Name: membrane bound O-acyltransferase domain containing 2
Synonyms: 2810049G06Rik, Oact2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 67216
VEGA: 12
Homologene: 6971
Medag
Name: mesenteric estrogen dependent adipogenesis
Synonyms: MEDA-4, 6330406I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70717
Homologene: 12364
Pwwp4a
Name: PWWP domain containing 4A
Synonyms: Gm14685
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 574404
Homologene: 128534
Ghdc
Name: GH3 domain containing
Synonyms: D11Lgp1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 80860
Homologene: 32760
Cep295nl
Name: CEP295 N-terminal like
Synonyms: Ddc8, BC100451
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 58251
Homologene: 75164
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 21,402,204 bp
  • T to C, chromosome 1 at 93,060,795 bp
  • T to A, chromosome 2 at 25,892,360 bp
  • T to A, chromosome 2 at 86,508,745 bp
  • A to G, chromosome 2 at 120,692,868 bp
  • T to C, chromosome 2 at 151,654,701 bp
  • A to G, chromosome 2 at 153,684,487 bp
  • C to T, chromosome 3 at 36,908,211 bp
  • C to T, chromosome 3 at 73,049,964 bp
  • T to C, chromosome 3 at 154,258,493 bp
  • A to G, chromosome 4 at 26,327,871 bp
  • C to T, chromosome 4 at 28,938,686 bp
  • G to A, chromosome 4 at 43,681,300 bp
  • C to T, chromosome 4 at 63,149,429 bp
  • T to C, chromosome 5 at 14,677,080 bp
  • G to A, chromosome 5 at 20,902,370 bp
  • G to A, chromosome 5 at 27,850,889 bp
  • T to A, chromosome 5 at 30,388,772 bp
  • T to C, chromosome 5 at 121,253,858 bp
  • C to G, chromosome 5 at 122,127,565 bp
  • T to A, chromosome 5 at 149,427,252 bp
  • G to A, chromosome 6 at 47,963,871 bp
  • A to C, chromosome 6 at 52,170,402 bp
  • A to T, chromosome 7 at 4,942,714 bp
  • G to T, chromosome 7 at 11,847,375 bp
  • A to T, chromosome 7 at 17,747,247 bp
  • A to T, chromosome 7 at 43,047,459 bp
  • C to T, chromosome 7 at 43,454,563 bp
  • T to C, chromosome 7 at 46,056,727 bp
  • CG to CGACGGCGGTG, chromosome 7 at 97,579,908 bp
  • G to A, chromosome 7 at 106,423,231 bp
  • T to C, chromosome 7 at 122,990,352 bp
  • T to C, chromosome 8 at 44,952,393 bp
  • A to G, chromosome 8 at 105,020,345 bp
  • A to G, chromosome 9 at 22,333,052 bp
  • A to G, chromosome 9 at 32,116,140 bp
  • A to G, chromosome 9 at 40,022,689 bp
  • A to G, chromosome 9 at 66,486,078 bp
  • C to T, chromosome 10 at 80,309,160 bp
  • T to C, chromosome 11 at 49,645,997 bp
  • A to G, chromosome 11 at 67,038,229 bp
  • A to C, chromosome 11 at 67,222,408 bp
  • A to G, chromosome 11 at 74,121,580 bp
  • A to G, chromosome 11 at 100,583,588 bp
  • T to C, chromosome 11 at 100,769,192 bp
  • G to A, chromosome 11 at 106,312,453 bp
  • T to C, chromosome 11 at 118,333,089 bp
  • A to G, chromosome 12 at 24,946,673 bp
  • T to A, chromosome 12 at 84,009,753 bp
  • A to G, chromosome 12 at 85,329,903 bp
  • T to C, chromosome 12 at 110,666,423 bp
  • G to C, chromosome 13 at 13,002,201 bp
  • C to G, chromosome 13 at 22,278,764 bp
  • T to C, chromosome 13 at 24,962,647 bp
  • A to G, chromosome 13 at 41,118,304 bp
  • T to A, chromosome 14 at 60,293,407 bp
  • T to A, chromosome 14 at 86,984,866 bp
  • T to C, chromosome 15 at 6,416,917 bp
  • T to A, chromosome 15 at 9,668,903 bp
  • T to G, chromosome 15 at 44,499,982 bp
  • C to T, chromosome 15 at 98,058,315 bp
  • T to C, chromosome 15 at 101,529,584 bp
  • C to T, chromosome 15 at 102,222,118 bp
  • C to T, chromosome 19 at 5,953,157 bp
  • C to G, chromosome 19 at 55,180,999 bp
  • T to A, chromosome X at 8,641,765 bp
  • G to T, chromosome X at 73,127,655 bp
  • C to T, chromosome X at 106,420,705 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1999 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040009-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.