MMRRC:040136-MU

T to A, chromosome 1 at 21,226,059 bp
T to G, chromosome 1 at 40,607,741 bp
A to G, chromosome 1 at 58,169,843 bp
ACTCTCTCTCTCTCTCT to ACTCTCTCTCTCTCTCTCT, chromosome 1 at 131,663,285 bp
C to A, chromosome 1 at 133,279,365 bp
AGTG to AGTGGCACCTTTGGTG, chromosome 1 at 133,327,321 bp
C to A, chromosome 1 at 133,358,982 bp
G to A, chromosome 1 at 133,387,071 bp
A to T, chromosome 1 at 133,903,796 bp
C to T, chromosome 1 at 133,915,131 bp
ACTCTCTCT to ACTCTCTCTCT, chromosome 1 at 134,746,741 bp
AG to AGG, chromosome 1 at 134,971,364 bp
ATCCTCCTCCTCCTCCTC to ATCCTCCTCCTCCTCCTCCTC, chromosome 1 at 135,386,268 bp
TCC to TCCACC, chromosome 1 at 135,386,275 bp
A to G, chromosome 1 at 135,402,250 bp
TG to TGG, chromosome 1 at 135,846,761 bp
AGGG to AGG, chromosome 1 at 135,974,852 bp
CC to CCGAC, chromosome 1 at 136,490,395 bp
T to C, chromosome 1 at 143,760,034 bp
C to T, chromosome 1 at 155,225,786 bp
A to G, chromosome 2 at 36,480,047 bp
A to T, chromosome 2 at 37,378,916 bp
T to A, chromosome 2 at 49,758,805 bp
A to T, chromosome 2 at 69,323,883 bp
T to A, chromosome 2 at 76,586,214 bp
G to A, chromosome 2 at 76,850,612 bp
G to A, chromosome 2 at 77,059,607 bp
G to A, chromosome 2 at 89,575,256 bp
T to C, chromosome 2 at 103,981,062 bp
A to G, chromosome 2 at 128,967,830 bp
T to A, chromosome 2 at 135,325,667 bp
A to T, chromosome 2 at 156,095,510 bp
A to G, chromosome 2 at 168,940,743 bp
T to C, chromosome 3 at 40,970,535 bp
A to T, chromosome 3 at 75,908,149 bp
G to A, chromosome 3 at 87,810,572 bp
T to A, chromosome 3 at 103,735,869 bp
T to A, chromosome 3 at 108,351,686 bp
T to A, chromosome 3 at 116,539,124 bp
C to A, chromosome 4 at 62,327,899 bp
A to T, chromosome 4 at 89,688,709 bp
A to G, chromosome 4 at 100,410,025 bp
A to G, chromosome 4 at 109,710,845 bp
A to T, chromosome 4 at 114,610,008 bp
A to T, chromosome 4 at 115,491,391 bp
T to C, chromosome 4 at 119,657,631 bp
A to T, chromosome 4 at 124,785,168 bp
A to T, chromosome 4 at 129,596,926 bp
T to A, chromosome 4 at 141,005,016 bp
G to A, chromosome 4 at 141,928,400 bp
C to T, chromosome 5 at 86,906,557 bp
TGGGG to TGGG, chromosome 5 at 114,209,767 bp
G to A, chromosome 5 at 134,136,153 bp
C to T, chromosome 5 at 144,174,988 bp
T to A, chromosome 5 at 144,208,645 bp
T to C, chromosome 5 at 151,045,168 bp
T to C, chromosome 6 at 33,758,158 bp
T to C, chromosome 6 at 33,910,538 bp
C to A, chromosome 6 at 42,744,135 bp
T to C, chromosome 6 at 47,298,445 bp
A to G, chromosome 6 at 89,145,469 bp
C to T, chromosome 6 at 90,626,381 bp
T to C, chromosome 6 at 124,449,503 bp
T to C, chromosome 6 at 134,248,754 bp
T to A, chromosome 7 at 16,081,663 bp
T to A, chromosome 7 at 19,068,106 bp
T to A, chromosome 7 at 27,654,349 bp
T to C, chromosome 7 at 35,453,493 bp
A to T, chromosome 7 at 46,869,599 bp
T to A, chromosome 7 at 48,824,644 bp
A to T, chromosome 7 at 62,377,738 bp
G to T, chromosome 7 at 75,611,434 bp
G to A, chromosome 7 at 78,477,935 bp
C to T, chromosome 7 at 118,382,047 bp
T to C, chromosome 7 at 118,794,575 bp
T to A, chromosome 7 at 135,704,241 bp
C to A, chromosome 7 at 143,592,474 bp
T to C, chromosome 8 at 22,011,077 bp
A to T, chromosome 8 at 25,164,493 bp
C to A, chromosome 8 at 71,352,162 bp
A to T, chromosome 8 at 72,386,868 bp
A to T, chromosome 8 at 111,297,402 bp
A to T, chromosome 8 at 128,498,516 bp
C to T, chromosome 9 at 22,358,604 bp
T to C, chromosome 9 at 45,803,183 bp
T to A, chromosome 9 at 53,149,512 bp
T to C, chromosome 9 at 72,308,005 bp
G to A, chromosome 10 at 5,346,808 bp
A to G, chromosome 10 at 11,343,682 bp
T to C, chromosome 10 at 78,401,894 bp
T to A, chromosome 10 at 81,278,054 bp
A to G, chromosome 10 at 120,165,177 bp
T to G, chromosome 10 at 127,011,892 bp
T to A, chromosome 11 at 3,680,302 bp
T to C, chromosome 11 at 5,977,880 bp
T to A, chromosome 11 at 67,096,864 bp
CAAATAAATAAATAAATAAATAAATAAATAAATAAA to CAAATAAATAAATAAATAAATAAATAAATAAATAAATAAA, chromosome 11 at 68,829,866 bp
G to C, chromosome 11 at 76,211,050 bp
G to A, chromosome 11 at 78,540,034 bp
A to T, chromosome 11 at 87,583,436 bp
T to A, chromosome 11 at 87,821,130 bp
A to G, chromosome 11 at 101,775,417 bp
G to A, chromosome 11 at 102,064,595 bp
T to A, chromosome 11 at 107,670,484 bp
A to G, chromosome 11 at 115,881,791 bp
A to G, chromosome 11 at 116,358,694 bp
T to C, chromosome 12 at 17,321,611 bp
A to T, chromosome 12 at 28,746,598 bp
T to A, chromosome 12 at 79,268,434 bp
T to C, chromosome 12 at 102,394,908 bp
A to G, chromosome 12 at 112,852,403 bp
T to C, chromosome 13 at 34,913,680 bp
T to A, chromosome 13 at 107,743,987 bp
T to A, chromosome 14 at 12,211,637 bp
A to G, chromosome 14 at 27,476,614 bp
T to C, chromosome 14 at 32,325,934 bp
T to A, chromosome 14 at 64,233,928 bp
T to C, chromosome 14 at 70,638,487 bp
A to T, chromosome 14 at 77,794,856 bp
A to T, chromosome 14 at 99,079,877 bp
T to C, chromosome 15 at 44,516,185 bp
T to A, chromosome 15 at 74,529,908 bp
A to G, chromosome 15 at 82,618,501 bp
A to G, chromosome 15 at 83,505,235 bp
T to A, chromosome 15 at 98,075,967 bp
A to T, chromosome 15 at 99,243,375 bp
G to A, chromosome 16 at 16,053,273 bp
A to G, chromosome 17 at 25,383,528 bp
G to A, chromosome 17 at 34,879,902 bp
T to C, chromosome 17 at 43,624,833 bp
T to C, chromosome 17 at 46,161,202 bp
A to G, chromosome 17 at 48,090,452 bp
T to C, chromosome 17 at 52,893,933 bp
A to T, chromosome 17 at 57,027,832 bp
G to A, chromosome 18 at 13,844,705 bp
T to C, chromosome 18 at 33,874,195 bp
C to A, chromosome 18 at 34,312,045 bp
C to A, chromosome 18 at 37,447,870 bp
T to C, chromosome 18 at 60,602,895 bp
T to C, chromosome 18 at 80,117,308 bp
T to C, chromosome 18 at 82,621,304 bp
C to A, chromosome 19 at 10,864,066 bp
A to G, chromosome 19 at 59,464,033 bp
C to A, chromosome X at 71,119,392 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Suggested Control Mice

Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R2133 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
040136-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All