Strain Name:
C57BL/6J-MtgxR2220Btlr/Mmmh
Stock Number:
040222-MU
Citation ID:
RRID:MMRRC_040222-MU
Other Names:
R2220 (G1), C57BL/6J-MtgxR2220Btlr
Major Collection:

Strain Information

Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, separase, SSE, ESP1, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Gdf6
Name: growth differentiation factor 6
Synonyms: BMP13
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242316
HGNC: HGNC:4221
Homologene: 40883
Ppp1r37
Name: protein phosphatase 1, regulatory subunit 37
Synonyms: Lrrc68
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232947
Homologene: 17851
Ppp2r3c
Name: protein phosphatase 2, regulatory subunit B'', gamma
Synonyms: 5730412A08Rik, G4-1, G5pr, 4930511A21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 59032
VEGA: 12
Homologene: 9915
Ggnbp2
Name: gametogenetin binding protein 2
Synonyms: DIF-3, Zfp403, D330017P12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217039
Homologene: 11750
Ltbp4
Name: latent transforming growth factor beta binding protein 4
Synonyms: 2310046A13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 108075
HGNC: HGNC:6717
Homologene: 2645
Cdkal1
Name: CDK5 regulatory subunit associated protein 1-like 1
Synonyms: 6620401C13Rik, 1190005B03Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68916
Homologene: 9830
Bltp1
Name: bridge-like lipid transfer protein family member 1
Synonyms: Tweek, FSA, 4932438A13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229227
Homologene: 52105
Cep85
Name: centrosomal protein 85
Synonyms: 2410030J07Rik, Ccdc21
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 70012
Homologene: 11254
Lars2
Name: leucyl-tRNA synthetase, mitochondrial
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102436
VEGA: 9
Homologene: 6526
Supt16
Name: SPT16, facilitates chromatin remodeling subunit
Synonyms: Cdc68, Fact140, Spt16, Supt16h
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 114741
VEGA: 14
Homologene: 5207
Cluh
Name: clustered mitochondria homolog
Synonyms: 1300001I01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74148
Homologene: 9063
Ap5m1
Name: adaptor-related protein complex 5, mu 1 subunit
Synonyms: 4932432K03Rik, Mudeng
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 74385
VEGA: 14
Homologene: 10081
Tasor2
Name: transcription activation suppressor family member 2
Synonyms: BC016423, Fam208b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105203
VEGA: 13
Homologene: 26435
Bcl6
Name: B cell leukemia/lymphoma 6
Synonyms: Bcl5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12053
HGNC: HGNC:1001
Homologene: 7640
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: LOC381341, Dnahc7, Dnahc7a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 627872
Homologene: 41287
Bicc1
Name: BicC family RNA binding protein 1
Synonyms: Bic-C, jcpk
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 83675
Homologene: 12856
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230895
Homologene: 15583
Dusp3
Name: dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related)
Synonyms: T-DSP11, VHR, 2210015O03Rik, 5031436O03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72349
HGNC: HGNC:3069
Homologene: 20870
Ppp3ca
Name: protein phosphatase 3, catalytic subunit, alpha isoform
Synonyms: PP2B alpha 1, PP2BA alpha, CnA, Caln, Calna, 2900074D19Rik, CN
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19055
HGNC: HGNC:9314
Homologene: 55497
Cops8
Name: COP9 signalosome subunit 8
Synonyms: Csn8, 9430009J09Rik, Sgn8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108679
Homologene: 4882
Pcdhb16
Name: protocadherin beta 16
Synonyms: PcdhbP, Pcdhb8
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93887
Homologene: 99843
Iqcb1
Name: IQ calmodulin-binding motif containing 1
Synonyms: 6820449I09Rik, NPHP5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 320299
Homologene: 8766
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94109
Homologene: 69536
Rpgrip1l
Name: Rpgrip1-like
Synonyms: Ftm, 1700047E16Rik, fantom, Nphp8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244585
Homologene: 18296
Uggt2
Name: UDP-glucose glycoprotein glucosyltransferase 2
Synonyms: 3110001A05Rik, 1810064L21Rik, 3110027P15Rik, A230065J02Rik, Ugcgl2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66435
Homologene: 56875
4930432E11Rik
Name: RIKEN cDNA 4930432E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243900
Tecta
Name: tectorin alpha
Synonyms: [a]-tectorin, Tctna
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21683
Homologene: 3955
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 672511
Homologene: 45439
Insrr
Name: insulin receptor-related receptor
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 23920
HGNC: HGNC:6093
Homologene: 56539
Ralgapa2
Name: Ral GTPase activating protein, alpha subunit 2 (catalytic)
Synonyms: A230067G21Rik, RGC2, AS250
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241694
Homologene: 28131
Ermap
Name: erythroblast membrane-associated protein
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 27028
Homologene: 22782
Cyb5d1
Name: cytochrome b5 domain containing 1
Synonyms: LOC327951
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327951
Homologene: 15945
Ggt7
Name: gamma-glutamyltransferase 7
Synonyms: 1110017C11Rik, 6330563L03Rik, Ggtl3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 207182
HGNC: HGNC:4259
Homologene: 70866
Fer1l4
Name: fer-1 like family member 4
Synonyms: 9130402C12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74562
Homologene: 19075
Aox2
Name: aldehyde oxidase 2
Synonyms: Aox3l1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213043
Homologene: 84622
Aadacl4
Name: arylacetamide deacetylase like 4
Synonyms: Gm13177
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 435815
Homologene: 82530
Or5k1
Name: olfactory receptor family 5 subfamily K member 1
Synonyms: GA_x54KRFPKG5P-54960233-54959268, MOR184-3, Olfr173
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 259002
Homologene: 45084
Mast3
Name: microtubule associated serine/threonine kinase 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 546071
Homologene: 66191
Pard3b
Name: par-3 family cell polarity regulator beta
Synonyms: 2810455B10Rik, PAR3beta, PAR3L, PAR3B, 2010002N16Rik, Als2cr19, 1810008K04Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72823
Homologene: 35389
Ttc23l
Name: tetratricopeptide repeat domain 23-like
Synonyms: 4930401A09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75777
Homologene: 44907
Abca8a
Name: ATP-binding cassette, sub-family A member 8a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217258
HGNC: HGNC:38
Homologene: 131160
Syde2
Name: synapse defective 1, Rho GTPase, homolog 2 (C. elegans)
Synonyms: C430017H16Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 214804
Homologene: 19035
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: B230363K08Rik, Ccdc108
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241116
Homologene: 28093
Flg2
Name: filaggrin family member 2
Synonyms: EG229574
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229574
Homologene: 134146
Dhx30
Name: DExH-box helicase 30
Synonyms: Ddx30, 2810477H02Rik, C130058C04Rik, helG, DEAH (Asp-Glu-Ala-His) box polypeptide 30
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72831
Homologene: 15779
Aatk
Name: apoptosis-associated tyrosine kinase
Synonyms: AATYK1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11302
HGNC: HGNC:21
Homologene: 74861
Mertk
Name: MER proto-oncogene tyrosine kinase
Synonyms: Tyro 12, Nyk, Eyk, Mer, nmf12
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17289
HGNC: HGNC:7027
Homologene: 4626
Klhdc7a
Name: kelch domain containing 7A
Synonyms: B230308G19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242721
Homologene: 17567
Ccdc83
Name: coiled-coil domain containing 83
Synonyms: 4930549K11Rik, 4930554C01Rik, 4932423M01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75338
Homologene: 32709
Trim30c
Name: tripartite motif-containing 30C
Synonyms: Trim30-2, Gm5598
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434219
Tmem174
Name: transmembrane protein 174
Synonyms: 0610009B10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68344
VEGA: 13
Homologene: 17711
Or13a19
Name: olfactory receptor family 13 subfamily A member 19
Synonyms: GA_x6K02T2PBJ9-42472898-42473827, MOR251-2, Olfr525
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258958
Homologene: 138321
Cntnap5a
Name: contactin associated protein-like 5A
Synonyms: Caspr5-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 636808
Homologene: 43974
Stt3a
Name: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Synonyms: Itm1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 16430
HGNC: HGNC:6172
Homologene: 40617
Hivep3
Name: human immunodeficiency virus type I enhancer binding protein 3
Synonyms: Krc, E030045D18Rik, 2900056N03Rik, Shn3, Schnurri-3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16656
Homologene: 7803
Cfap61
Name: cilia and flagella associated protein 61
Synonyms: 4930529M08Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78774
Cyp2c29
Name: cytochrome P450, family 2, subfamily c, polypeptide 29
Synonyms: AHOH, AHOHase, Ahh-1, Ah-2, P450-2C, Cyp2c
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13095
Homologene: 117948
Clca4c-ps
Name: chloride channel accessory 4C, pseudogene
Synonyms: Gm6289, Clca4c, Gm18718
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 622139
Or2l13b
Name: olfactory receptor family 2 subfamily L member 13B
Synonyms: GA_x54KRFPKG5P-15979009-15978071, MOR271-1, Olfr168
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258354
Homologene: 128057
Igsf21
Name: immunoglobulin superfamily, member 21
Synonyms: LOC230868
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230868
Homologene: 13153
Cyp2j8
Name: cytochrome P450, family 2, subfamily j, polypeptide 8
Synonyms: OTTMUSG00000007938, Cyp2j8-ps
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 665095
HGNC: HGNC:2634
Homologene: 133819
Pira12
Name: paired-Ig-like receptor A12
Synonyms: Gm14548
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100038909
Homologene: 134028
Ptgds
Name: prostaglandin D2 synthase (brain)
Synonyms: PGD2, L-PGDS, Ptgs3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19215
HGNC: HGNC:9592
Homologene: 737
Slc25a18
Name: solute carrier family 25 (mitochondrial carrier), member 18
Synonyms: 1500015I14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 71803
Homologene: 57151
Tmc7
Name: transmembrane channel-like gene family 7
Synonyms: C630024K23Rik, 1700030H01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 209760
Homologene: 36424
Tomm40l
Name: translocase of outer mitochondrial membrane 40-like
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 641376
Homologene: 134180
Slc11a1
Name: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
Synonyms: host resistance locus Bcg/Ity/Lsh, Nramp, Lsh, Bcg, Ity, Nramp1, ity, Ity1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18173
Homologene: 73884
Wfdc18
Name: WAP four-disulfide core domain 18
Synonyms: WDNM1, Kal1, Expi
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14038
Mettl21a
Name: methyltransferase 21A, HSPA lysine
Synonyms: 2310038H17Rik, Fam119a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67099
Homologene: 41658
Gm3150
Name: predicted gene 3150
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 100041120
Gtf2h5
Name: general transcription factor IIH, polypeptide 5
Synonyms: 2700017P07Rik, 2810432H05Rik, D17Wsu155e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66467
Homologene: 45635
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 53,521,174 bp
  • A to G, chromosome 1 at 58,349,130 bp
  • C to T, chromosome 1 at 62,479,683 bp
  • A to T, chromosome 1 at 64,616,283 bp
  • T to A, chromosome 1 at 74,380,665 bp
  • T to C, chromosome 1 at 74,904,025 bp
  • A to C, chromosome 1 at 90,606,619 bp
  • A to T, chromosome 1 at 116,580,639 bp
  • A to T, chromosome 1 at 171,221,981 bp
  • T to C, chromosome 2 at 25,467,804 bp
  • T to A, chromosome 2 at 128,801,472 bp
  • G to A, chromosome 2 at 146,036,816 bp
  • G to A, chromosome 2 at 146,421,679 bp
  • A to G, chromosome 2 at 155,495,719 bp
  • T to G, chromosome 2 at 156,031,764 bp
  • T to C, chromosome 3 at 36,875,530 bp
  • T to C, chromosome 3 at 87,809,418 bp
  • T to G, chromosome 3 at 93,202,185 bp
  • C to A, chromosome 3 at 136,797,924 bp
  • T to A, chromosome 3 at 144,879,469 bp
  • A to G, chromosome 3 at 146,001,958 bp
  • A to G, chromosome 4 at 9,844,770 bp
  • T to C, chromosome 4 at 96,444,625 bp
  • A to T, chromosome 4 at 119,188,343 bp
  • T to G, chromosome 4 at 119,734,038 bp
  • T to C, chromosome 4 at 134,153,867 bp
  • G to A, chromosome 4 at 139,965,453 bp
  • A to G, chromosome 4 at 140,028,114 bp
  • T to C, chromosome 4 at 144,618,002 bp
  • C to T, chromosome 4 at 145,178,320 bp
  • G to A, chromosome 6 at 120,793,557 bp
  • A to T, chromosome 7 at 3,897,489 bp
  • C to A, chromosome 7 at 19,532,446 bp
  • A to T, chromosome 7 at 27,335,384 bp
  • T to A, chromosome 7 at 29,562,818 bp
  • G to A, chromosome 7 at 90,259,514 bp
  • A to G, chromosome 7 at 104,383,267 bp
  • A to T, chromosome 7 at 118,552,816 bp
  • T to C, chromosome 7 at 140,323,571 bp
  • T to C, chromosome 8 at 15,992,641 bp
  • T to C, chromosome 8 at 70,780,963 bp
  • A to G, chromosome 8 at 91,300,769 bp
  • A to G, chromosome 9 at 36,749,551 bp
  • T to C, chromosome 9 at 42,392,030 bp
  • T to A, chromosome 9 at 72,736,707 bp
  • A to G, chromosome 9 at 110,087,635 bp
  • T to C, chromosome 9 at 123,418,780 bp
  • A to G, chromosome 10 at 70,950,125 bp
  • T to C, chromosome 11 at 69,395,045 bp
  • T to A, chromosome 11 at 74,667,121 bp
  • C to T, chromosome 11 at 83,709,913 bp
  • T to C, chromosome 11 at 84,836,613 bp
  • T to C, chromosome 11 at 101,974,805 bp
  • A to T, chromosome 11 at 110,026,855 bp
  • T to C, chromosome 11 at 119,436,428 bp
  • G to T, chromosome 11 at 120,012,177 bp
  • A to G, chromosome 12 at 55,288,064 bp
  • A to T, chromosome 13 at 3,581,872 bp
  • T to A, chromosome 13 at 29,354,758 bp
  • T to C, chromosome 13 at 98,637,259 bp
  • A to G, chromosome 14 at 49,081,095 bp
  • G to A, chromosome 14 at 52,172,144 bp
  • A to T, chromosome 14 at 119,075,337 bp
  • G to A, chromosome 15 at 10,537,566 bp
  • A to T, chromosome 15 at 72,913,430 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • A to T, chromosome 16 at 19,530,145 bp
  • A to T, chromosome 16 at 23,972,632 bp
  • A to T, chromosome 16 at 36,843,462 bp
  • G to T, chromosome 16 at 58,797,624 bp
  • G to A, chromosome 17 at 6,084,578 bp
  • A to G, chromosome 18 at 37,478,967 bp
  • A to T, chromosome 19 at 39,287,232 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2220 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040222-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.