Strain Name:
C57BL/6J-MtgxR2284Btlr/Mmmh
Stock Number:
040283-MU
Citation ID:
RRID:MMRRC_040283-MU
Other Names:
R2284 (G1), C57BL/6J-MtgxR2284Btlr
Major Collection:

Gene Information

Usp7
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 252870
Homologene: 2592
Adsl
Name: adenylosuccinate lyase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11564
HGNC: HGNC:291
Homologene: 12
Cryzl1
Name: crystallin, zeta (quinone reductase)-like 1
Synonyms: 2210407J23Rik, 2410006O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66609
HGNC: HGNC:2420
Homologene: 3749
Mtmr12
Name: myotubularin related protein 12
Synonyms: C730015A02Rik, Pip3ap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 268783
Homologene: 10403
Sacm1l
Name: SAC1 suppressor of actin mutations 1-like (yeast)
Synonyms: Sac1p, SAC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 83493
VEGA: 9
Homologene: 6320
Mki67
Name: antigen identified by monoclonal antibody Ki 67
Synonyms: Ki-67, D630048A14Rik, Ki67
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17345
HGNC: HGNC:7107
Homologene: 1814
Map2
Name: microtubule-associated protein 2
Synonyms: MAP-2, G1-397-34, Mtap2, repro4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17756
HGNC: HGNC:6839
Homologene: 1779
Osbpl7
Name: oxysterol binding protein-like 7
Synonyms: 4933437E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71240
Homologene: 79672
Ccdc88a
Name: coiled coil domain containing 88A
Synonyms: C330012F17Rik, D130005J21Rik, C130096N06Rik, GIV, HkRP1, Girdin, A430106J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108686
Homologene: 41180
Tsc1
Name: TSC complex subunit 1
Synonyms: hamartin, tuberous sclerosis 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 64930
Homologene: 314
Nr2f1
Name: nuclear receptor subfamily 2, group F, member 1
Synonyms: COUP-TFI, Erbal3, Tcfcoup1, COUP-TF1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13865
HGNC: HGNC:7975
Homologene: 21158
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328783
VEGA: 17
Homologene: 18633
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Nlrp1b
Name: NLR family, pyrin domain containing 1B
Synonyms: Nalp1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 637515
Homologene: 19080
Naip6
Name: NLR family, apoptosis inhibitory protein 6
Synonyms: Naip-rs4A, Naip-rs4, Birc1f
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17952
HGNC: HGNC:7634
Homologene: 113589
Vmn1r67
Name: vomeronasal 1 receptor 67
Synonyms: V1re10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171263
Homologene: 128344
Klrd1
Name: killer cell lectin-like receptor, subfamily D, member 1
Synonyms: CD94
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16643
HGNC: HGNC:6378
Homologene: 1705
Gm5698
Name: predicted gene 5698
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435615
Gtf2ird2
Name: GTF2I repeat domain containing 2
Synonyms: 1700012P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 114674
Homologene: 24941
Nrxn3
Name: neurexin III
Synonyms: neurexin III beta, neurexin III alpha, neurexin III beta, neurexin III alpha, 9330112C09Rik, D12Bwg0831e, 4933401A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Wt1
Name: WT1 transcription factor
Synonyms: Wt-1, D630046I19Rik, Wilms tumor 1 homolog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22431
Homologene: 11536
Iho1
Name: interactor of HORMAD1 1
Synonyms: Ccdc36, Iho1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 434438
Homologene: 52256
Ptpre
Name: protein tyrosine phosphatase, receptor type, E
Synonyms: PTPe, PTPepsilon, RPTPepsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19267
HGNC: HGNC:9669
Homologene: 31387
Adipoq
Name: adiponectin, C1Q and collagen domain containing
Synonyms: adiponectin, adipo, apM1, GBP28, Acrp30, Acdc, APN, Ad
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11450
Homologene: 3525
Hdac10
Name: histone deacetylase 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 170787
Homologene: 23749
Hhatl
Name: hedgehog acyltransferase-like
Synonyms: 1110011D13Rik, Gup1, Mitsugumin 56, Mg56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74770
VEGA: 9
Homologene: 19287
Dip2a
Name: disco interacting protein 2 homolog A
Synonyms: Kiaa0184-hp, 4931420H10Rik, Dip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64451
Homologene: 41012
Vmn2r45
Name: vomeronasal 2, receptor 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042810
Homologene: 113703
Necab2
Name: N-terminal EF-hand calcium binding protein 2
Synonyms: Necab2, Efcbp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 117148
Homologene: 62200
Vmn1r42
Name: vomeronasal 1 receptor 42
Synonyms: V1ra6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 113848
Homologene: 130651
Olfr1157
Name: olfactory receptor 1157
Synonyms: GA_x6K02T2Q125-49446395-49445457, MOR174-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258846
HGNC: HGNC:8350
Homologene: 17402
Krtap31-1
Name: keratin associated protein 31-1
Synonyms: 4733401H21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70831
Homologene: 134336
Bcas2
Name: BCAS2 pre-mRNA processing factor
Synonyms: 6430539P16Rik, C76366, breast carcinoma amplified sequence 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68183
HGNC: HGNC:975
Homologene: 4291
Gm5830
Name: predicted pseudogene 5830
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545332
Gldn
Name: gliomedin
Synonyms: Crlg2, CRG-L2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235379
Homologene: 14529
Csl
Name: citrate synthase like
Synonyms: 1700007H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71832
VEGA: 10
Homologene: 7131
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 30,977,883 bp
  • C to T, chromosome 1 at 66,414,068 bp
  • A to T, chromosome 1 at 78,967,698 bp
  • G to A, chromosome 2 at 28,665,097 bp
  • A to G, chromosome 2 at 87,962,793 bp
  • A to G, chromosome 2 at 105,172,321 bp
  • T to C, chromosome 3 at 103,178,362 bp
  • A to G, chromosome 5 at 134,217,183 bp
  • A to G, chromosome 6 at 89,844,699 bp
  • A to G, chromosome 6 at 129,598,381 bp
  • A to T, chromosome 7 at 8,485,766 bp
  • A to T, chromosome 7 at 10,447,673 bp
  • A to G, chromosome 7 at 135,669,781 bp
  • A to G, chromosome 7 at 135,699,945 bp
  • T to C, chromosome 7 at 141,637,924 bp
  • G to A, chromosome 8 at 119,471,000 bp
  • G to A, chromosome 9 at 54,286,565 bp
  • C to T, chromosome 9 at 108,421,473 bp
  • T to C, chromosome 9 at 121,789,582 bp
  • T to A, chromosome 9 at 123,569,938 bp
  • A to C, chromosome 10 at 76,313,193 bp
  • T to A, chromosome 10 at 99,758,459 bp
  • T to C, chromosome 11 at 29,494,099 bp
  • T to C, chromosome 11 at 55,282,360 bp
  • A to G, chromosome 11 at 71,156,284 bp
  • G to A, chromosome 11 at 97,056,019 bp
  • C to T, chromosome 11 at 99,908,255 bp
  • T to A, chromosome 12 at 89,510,365 bp
  • C to A, chromosome 13 at 78,195,462 bp
  • C to T, chromosome 13 at 100,300,600 bp
  • T to A, chromosome 15 at 12,245,011 bp
  • C to T, chromosome 15 at 80,963,895 bp
  • T to C, chromosome 15 at 89,127,404 bp
  • T to C, chromosome 16 at 8,696,663 bp
  • C to T, chromosome 16 at 23,157,487 bp
  • C to T, chromosome 16 at 91,694,305 bp
  • G to A, chromosome 17 at 25,742,934 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2284 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040283-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.