Strain Name:
C57BL/6J-MtgxR2974Btlr/Mmmh
Stock Number:
040527-MU
Citation ID:
RRID:MMRRC_040527-MU
Other Names:
R2974 (G1), C57BL/6J-MtgxR2974Btlr
Major Collection:

Gene Information

Slit1
Name: slit guidance ligand 1
Synonyms: Slil1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20562
Homologene: 2302
Tpgs1
Name: tubulin polyglutamylase complex subunit 1
Synonyms: Gm16517, Gtrgeo22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 110012
VEGA: 10
Homologene: 17525
Phax
Name: phosphorylated adaptor for RNA export
Synonyms: Phax, p55, D18Ertd65e, 2810055C14Rik, Rnuxa, 4933427L19Rik, phosphorylation regulated
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 56698
Homologene: 10558
Rgs3
Name: regulator of G-protein signaling 3
Synonyms: C2pa, C2PA-RGS3, RGS3S, PDZ-RGS3, 4930506N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 50780
HGNC: HGNC:9999
Homologene: 32440
Cop1
Name: COP1, E3 ubiquitin ligase
Synonyms: Cop1, Rfwd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 26374
Homologene: 115565
Depdc5
Name: DEP domain containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 277854
Homologene: 34718
Gse1
Name: genetic suppressor element 1, coiled-coil protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382034
Homologene: 40964
Med15
Name: mediator complex subunit 15
Synonyms: A230074L19Rik, Pcqap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 94112
VEGA: 16
Tnip1
Name: TNFAIP3 interacting protein 1
Synonyms: Nef, A20-binding inhibitor of NF-kappa B activation, ABIN1, VAN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 57783
Homologene: 31355
Crnkl1
Name: crooked neck pre-mRNA splicing factor 1
Synonyms: 1200013P10Rik, crn, 5730590A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66877
Homologene: 6462
Slc25a27
Name: solute carrier family 25, member 27
Synonyms: 9430092A03Rik, 3632410G24Rik, Ucp4, D530043E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74011
VEGA: 17
Homologene: 12523
Trpv5
Name: transient receptor potential cation channel, subfamily V, member 5
Synonyms: CaT2, ECaC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 194352
HGNC: HGNC:3145
Homologene: 10520
Nyap2
Name: neuronal tyrosine-phophorylated phosphoinositide 3-kinase adaptor 2
Synonyms: 9430031J16Rik, Jr6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241134
Homologene: 18239
Dnah14
Name: dynein, axonemal, heavy chain 14
Synonyms: Dnahc14, A230079K17Rik, Gm980, LOC381311
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240960
HGNC: HGNC:2945
Homologene: 90078
Vmn2r77
Name: vomeronasal 2, receptor 77
Synonyms: EG546983
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 546983
Homologene: 115466
Gsdme
Name: gasdermin E
Synonyms: 2310037D07Rik, Fin15, 4932441K13Rik, Dfna5h, Dfna5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54722
HGNC: HGNC:2810
Homologene: 3242
Dpp10
Name: dipeptidylpeptidase 10
Synonyms: 6430601K09Rik, DPRP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269109
Homologene: 41400
Nlrp4d
Name: NLR family, pyrin domain containing 4D
Synonyms: Nalp-beta, Nalp4d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384752
Olfr948
Name: olfactory receptor 948
Synonyms: GA_x6K02T2PVTD-33016899-33015934, MOR171-51, MOR171-45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 257912
VEGA: 9
Homologene: 79377
Simc1
Name: SUMO-interacting motifs containing 1
Synonyms: 4732471D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 319719
Homologene: 131217
Olfr1182
Name: olfactory receptor 1182
Synonyms: Gm13757, MOR225-8P, GA_x6K02T2Q125-49934557-49933640, Olfr1536-ps1, MOR225-8P, MOR225-7P, MOR225-16_p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258167
Homologene: 86666
Serpinb9e
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9e
Synonyms: NK26, ovalbumin, Spi14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20710
HGNC: HGNC:8955
Homologene: 69093
Sptlc3
Name: serine palmitoyltransferase, long chain base subunit 3
Synonyms: C130053K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228677
Homologene: 105590
Ube2d2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Doxl2
Name: diamine oxidase-like protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243376
Homologene: 19443
Vmn2r117
Name: vomeronasal 2, receptor 117
Synonyms: EG619788, V2Rp6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 619788
Homologene: 86604
Rxrg
Name: retinoid X receptor gamma
Synonyms: Nr2b3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20183
Homologene: 21373
Abca7
Name: ATP-binding cassette, sub-family A (ABC1), member 7
Synonyms: Abc51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 27403
HGNC: HGNC:37
Homologene: 22783
Ctu1
Name: cytosolic thiouridylase subunit 1
Synonyms: Atpbd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233189
Homologene: 102140
Rogdi
Name: rogdi homolog
Synonyms: 0610011C19Rik, Lzf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66049
Homologene: 11605
BC048507
Name: cDNA sequence BC048507
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 408058
VEGA: 13
Homologene: 133712
Cstdc2
Name: cystatin domain containing 2
Synonyms: mCST E2, 9230104L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77705
Homologene: 87070
D930007J09Rik
Name: RIKEN cDNA D930007J09 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 81,191,770 bp
  • A to G, chromosome 1 at 123,411,705 bp
  • A to G, chromosome 1 at 159,324,929 bp
  • G to A, chromosome 1 at 167,639,146 bp
  • A to T, chromosome 1 at 181,755,241 bp
  • A to T, chromosome 2 at 88,446,574 bp
  • A to G, chromosome 2 at 139,589,661 bp
  • A to G, chromosome 2 at 145,932,261 bp
  • T to C, chromosome 2 at 148,850,786 bp
  • A to T, chromosome 4 at 62,640,720 bp
  • T to C, chromosome 5 at 32,934,017 bp
  • T to C, chromosome 6 at 41,653,231 bp
  • A to G, chromosome 6 at 48,976,424 bp
  • A to G, chromosome 6 at 50,229,324 bp
  • A to G, chromosome 7 at 10,378,440 bp
  • A to G, chromosome 7 at 43,675,650 bp
  • T to C, chromosome 7 at 86,803,685 bp
  • C to A, chromosome 8 at 120,570,897 bp
  • T to C, chromosome 9 at 39,318,996 bp
  • A to G, chromosome 10 at 79,669,615 bp
  • T to G, chromosome 10 at 80,008,967 bp
  • C to T, chromosome 11 at 54,933,983 bp
  • C to T, chromosome 13 at 32,802,759 bp
  • T to A, chromosome 13 at 33,255,143 bp
  • A to G, chromosome 13 at 54,550,461 bp
  • T to C, chromosome 13 at 67,863,630 bp
  • C to G, chromosome 15 at 76,188,761 bp
  • G to T, chromosome 16 at 5,011,662 bp
  • T to C, chromosome 16 at 17,652,711 bp
  • A to G, chromosome 17 at 23,459,856 bp
  • T to C, chromosome 17 at 43,653,371 bp
  • T to A, chromosome 18 at 35,800,172 bp
  • T to A, chromosome 18 at 56,573,062 bp
  • G to A, chromosome 19 at 41,611,016 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2974 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040527-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.