Strain Name:
C57BL/6J-MtgxR3002Btlr/Mmmh
Stock Number:
040531-MU
Citation ID:
RRID:MMRRC_040531-MU
Other Names:
R3002 (G1), C57BL/6J-MtgxR3002Btlr
Major Collection:

Gene Information

Nr4a1
Name: nuclear receptor subfamily 4, group A, member 1
Synonyms: Hmr, TR3, TIS1, Gfrp, N10, Nur77, GFRP1, NP10, Hbr-1, NGFI-B, Hbr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 15370
VEGA: 15
HGNC: HGNC:7980
Homologene: 1612
Flnb
Name: filamin, beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 286940
HGNC: HGNC:3755
Homologene: 37480
Tut4
Name: terminal uridylyl transferase 4
Synonyms: 9230115F04Rik, Zcchc11, Tent3a, 6030404K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230594
Homologene: 35279
Polr1a
Name: polymerase (RNA) I polypeptide A
Synonyms: RPA194, Rpo1-4, mRPA1, 3010014K16Rik, 194kDa, 2900087K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20019
Homologene: 7033
Slitrk5
Name: SLIT and NTRK-like family, member 5
Synonyms: 2610019D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75409
VEGA: 14
Homologene: 9193
Eif4g1
Name: eukaryotic translation initiation factor 4, gamma 1
Synonyms: E030015G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208643
HGNC: HGNC:3296
Homologene: 110725
Ncam1
Name: neural cell adhesion molecule 1
Synonyms: NCAM-140, NCAM, CD56, NCAM-120, NCAM-1, NCAM-180, E-NCAM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17967
HGNC: HGNC:7656
Homologene: 40754
Pon2
Name: paraoxonase 2
Synonyms: 6330405I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330260
HGNC: HGNC:9205
Homologene: 385
Knop1
Name: lysine rich nucleolar protein 1
Synonyms: 2310008H09Rik, Tsg118
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 66356
Homologene: 49729
Tmem68
Name: transmembrane protein 68
Synonyms: 2010300G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72098
Homologene: 12440
Tmeff2
Name: transmembrane protein with EGF-like and two follistatin-like domains 2
Synonyms: 4832418D20Rik, 7630402F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 56363
Homologene: 9417
Trpm2
Name: transient receptor potential cation channel, subfamily M, member 2
Synonyms: TRPC7, LTRPC2, Trrp7, 9830168K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 28240
Homologene: 20709
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Tex15
Name: testis expressed gene 15
Synonyms: 2210014E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104271
Homologene: 12837
Chrd
Name: chordin
Synonyms: Chd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12667
HGNC: HGNC:1949
Homologene: 2774
V1rd19
Name: vomeronasal 1 receptor, D19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404287
Homologene: 104166
Hal
Name: histidine ammonia lyase
Synonyms: histidase, Hsd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15109
HGNC: HGNC:4806
Homologene: 68229
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: E230008N13Rik, LOC381522
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381522
Homologene: 117988
Pik3ca
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Synonyms: caPI3K, p110alpha, 6330412C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18706
HGNC: HGNC:8975
Homologene: 21249
Folh1
Name: folate hydrolase 1
Synonyms: prostate-specific membrane antigen, glutamate carboxypeptidase II, mopsm, GCP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 53320
HGNC: HGNC:3788
Homologene: 55826
Il27ra
Name: interleukin 27 receptor, alpha
Synonyms: WSX-1, IL-27R, Tccr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 50931
Homologene: 3562
Adcy6
Name: adenylate cyclase 6
Synonyms: AC6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11512
VEGA: 15
HGNC: HGNC:237
Homologene: 22400
Sec14l5
Name: SEC14-like lipid binding 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 665119
VEGA: 16
Homologene: 96163
Slc2a4
Name: solute carrier family 2 (facilitated glucose transporter), member 4
Synonyms: twgy, Glut-4, Glut4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20528
Homologene: 74381
Rgl2
Name: ral guanine nucleotide dissociation stimulator-like 2
Synonyms: Rgt2, Rlf, Rab2l, KE1.5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19732
HGNC: HGNC:9769
Homologene: 3494
Otulinl
Name: OTU deubiquitinase with linear linkage specificity like
Synonyms: Fam105a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223433
VEGA: 15
Homologene: 41302
Tyk2
Name: tyrosine kinase 2
Synonyms: JTK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 54721
VEGA: 9
Homologene: 20712
Vmn2r24
Name: vomeronasal 2, receptor 24
Synonyms: EG243628
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243628
Homologene: 135915
Mindy4
Name: MINDY lysine 48 deubiquitinase 4
Synonyms: C330043M08Rik, Fam188b, LOC384387
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330323
Homologene: 49991
Thoc5
Name: THO complex 5
Synonyms: Fmip, 1700060C24Rik, A430085L24Rik, PK1.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 107829
Homologene: 37836
Mrgprb3
Name: MAS-related GPR, member B3
Synonyms: MrgB3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404238
Homologene: 138632
Hs3st2
Name: heparan sulfate (glucosamine) 3-O-sulfotransferase 2
Synonyms: 6430516N12Rik, A830061E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 195646
HGNC: HGNC:5195
Homologene: 21220
Cnksr3
Name: Cnksr family member 3
Synonyms: Magi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215748
Homologene: 18192
Pkd2l1
Name: polycystic kidney disease 2-like 1
Synonyms: TRPP3, Pkdl, PCL, polycystin-L, PKD2L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 329064
HGNC: HGNC:9011
Homologene: 22946
Slc17a1
Name: solute carrier family 17 (sodium phosphate), member 1
Synonyms: Npt1, NAPI-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20504
Homologene: 48324
Olfr446
Name: olfactory receptor 446
Synonyms: MOR261-12, GA_x6K02T2P3E9-4632269-4631343
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 258292
Homologene: 17179
Tgif2
Name: TGFB-induced factor homeobox 2
Synonyms: 5730599O09Rik, TALE family homeobox
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228839
Homologene: 32514
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 51,181,835 bp
  • C to G, chromosome 2 at 156,844,194 bp
  • T to A, chromosome 3 at 32,462,797 bp
  • A to C, chromosome 4 at 3,569,588 bp
  • A to G, chromosome 4 at 45,899,988 bp
  • G to A, chromosome 4 at 108,512,928 bp
  • A to G, chromosome 6 at 5,268,976 bp
  • A to G, chromosome 6 at 42,927,954 bp
  • T to A, chromosome 6 at 55,218,364 bp
  • A to G, chromosome 6 at 71,913,016 bp
  • T to A, chromosome 6 at 71,965,644 bp
  • A to C, chromosome 6 at 123,804,272 bp
  • A to T, chromosome 7 at 24,003,885 bp
  • C to T, chromosome 7 at 48,643,484 bp
  • A to C, chromosome 7 at 86,723,311 bp
  • CTCTTCTTCTTCTTCTTCTTCTTC to CTCTTCTTCTTCTTCTTC, chromosome 7 at 118,852,449 bp
  • T to A, chromosome 7 at 121,500,687 bp
  • A to G, chromosome 8 at 16,196,170 bp
  • T to C, chromosome 8 at 33,574,528 bp
  • G to T, chromosome 8 at 84,032,031 bp
  • C to A, chromosome 9 at 21,109,321 bp
  • A to G, chromosome 9 at 49,557,226 bp
  • T to C, chromosome 10 at 7,152,856 bp
  • A to T, chromosome 10 at 77,930,534 bp
  • G to A, chromosome 10 at 93,507,519 bp
  • A to G, chromosome 11 at 4,928,688 bp
  • A to T, chromosome 11 at 69,945,925 bp
  • G to A, chromosome 13 at 23,878,581 bp
  • G to T, chromosome 14 at 7,907,162 bp
  • A to G, chromosome 14 at 111,679,582 bp
  • T to C, chromosome 15 at 27,664,706 bp
  • T to C, chromosome 15 at 98,596,660 bp
  • A to G, chromosome 15 at 101,270,972 bp
  • A to G, chromosome 16 at 5,171,882 bp
  • T to A, chromosome 16 at 20,692,384 bp
  • T to G, chromosome 16 at 20,737,445 bp
  • A to G, chromosome 17 at 33,932,605 bp
  • A to G, chromosome 19 at 44,155,557 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3002 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040531-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.