Strain Name:
C57BL/6J-MtgxR3011Btlr/Mmmh
Stock Number:
040533-MU
Citation ID:
RRID:MMRRC_040533-MU
Other Names:
R3011 (G1), C57BL/6J-MtgxR3011Btlr
Major Collection:

Gene Information

Phf20
Name: PHD finger protein 20
Synonyms: 6820402O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228829
Homologene: 9507
Tmem59l
Name: transmembrane protein 59-like
Synonyms: 5330410G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67937
Homologene: 8104
Srgap2
Name: SLIT-ROBO Rho GTPase activating protein 2
Synonyms: Fnbp2, FBP2, 9930124L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14270
Homologene: 52683
Clasp2
Name: CLIP associating protein 2
Synonyms: CLASP2alpha, 1500004F14Rik, CLASP2, 8030404L10Rik, CLASP2gamma, CLASP2beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 76499
VEGA: 9
Homologene: 24944
Vps72
Name: vacuolar protein sorting 72
Synonyms: Tcfl1, YL-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 21427
Homologene: 110905
Clec16a
Name: C-type lectin domain family 16, member A
Synonyms: 4932416N17Rik, curt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 74374
Homologene: 71019
Dis3
Name: DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease
Synonyms: 2810028N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 72662
VEGA: 14
Homologene: 6910
Gm14403
Name: predicted gene 14403
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 433520
Otof
Name: otoferlin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 83762
HGNC: HGNC:8515
Homologene: 12892
Tmtc3
Name: transmembrane and tetratricopeptide repeat containing 3
Synonyms: B130008E12Rik, mSmile, 9130014E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237500
Homologene: 27616
Map2
Name: microtubule-associated protein 2
Synonyms: repro4, Mtap2, G1-397-34, MAP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 17756
HGNC: HGNC:6839
Homologene: 1779
Arhgap28
Name: Rho GTPase activating protein 28
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 268970
Homologene: 18264
Lct
Name: lactase
Synonyms: LOC226413, Lphl, LPH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 226413
HGNC: HGNC:6530
Homologene: 124204
Itga11
Name: integrin alpha 11
Synonyms: 4732459H24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 319480
HGNC: HGNC:6136
Homologene: 8151
Fam160b1
Name: family with sequence similarity 160, member B1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226252
VEGA: 19
Homologene: 28133
Gm884
Name: predicted gene 884
Synonyms: LOC380730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 380730
Homologene: 134511
Cped1
Name: cadherin-like and PC-esterase domain containing 1
Synonyms: A430107O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 214642
Homologene: 57014
Commd3
Name: COMM domain containing 3
Synonyms: D2Ertd542e, Bup
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12238
Homologene: 15693
Sppl2c
Name: signal peptide peptidase 2C
Synonyms: 4933407P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237958
Homologene: 18491
Olfr70
Name: olfactory receptor 70
Synonyms: GA_x6K02T2N78B-16239704-16240654, mOR6, MOR262-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 56014
Homologene: 10459
4930432K21Rik
Name: RIKEN cDNA 4930432K21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 74666
Homologene: 11525
Olfr190
Name: olfactory receptor 190
Synonyms: MOR183-4, GA_x54KRFPKG5P-55303207-55302284
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 258392
Homologene: 131129
Mgat4e
Name: MGAT4 family, member E
Synonyms: 4931440L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 71001
Homologene: 129994
Arhgap31
Name: Rho GTPase activating protein 31
Synonyms: CdGAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12549
Homologene: 10644
Nphp4
Name: nephronophthisis 4 (juvenile) homolog (human)
Synonyms: 4930564O18Rik, nmf192
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 260305
Homologene: 9024
Upp2
Name: uridine phosphorylase 2
Synonyms: 1700124F02Rik, UDRPASE2, UPASE2, UP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 76654
Homologene: 12654
Ifi204
Name: interferon activated gene 204
Synonyms: p204
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 15951
HGNC: HGNC:5395
Homologene: 115929
Dnajc5b
Name: DnaJ heat shock protein family (Hsp40) member C5 beta
Synonyms: 1700008A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 66326
Homologene: 32634
Gm5414
Name: predicted gene 5414
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 406223
VEGA: 15
Nqo1
Name: NAD(P)H dehydrogenase, quinone 1
Synonyms: Nmor1, Ox1, QR1, NQO1, Dia4, NAD(P)H dehydrogenase (quinone), Ox-1, NMO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 18104
HGNC: HGNC:2874
Homologene: 695
Snx18
Name: sorting nexin 18
Synonyms: Snag1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 170625
VEGA: 13
Homologene: 14164
Tex11
Name: testis expressed gene 11
Synonyms: 4930565P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 83558
Homologene: 49962
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 66,414,612 bp
  • C to T, chromosome 1 at 128,301,372 bp
  • T to A, chromosome 1 at 131,310,591 bp
  • T to A, chromosome 1 at 134,542,108 bp
  • T to C, chromosome 1 at 173,751,651 bp
  • T to A, chromosome 2 at 18,674,688 bp
  • T to C, chromosome 2 at 58,790,095 bp
  • A to G, chromosome 2 at 156,288,026 bp
  • A to G, chromosome 2 at 177,508,993 bp
  • A to T, chromosome 3 at 19,546,802 bp
  • A to G, chromosome 3 at 95,119,274 bp
  • G to T, chromosome 4 at 43,696,624 bp
  • C to T, chromosome 4 at 152,537,171 bp
  • G to A, chromosome 5 at 30,382,840 bp
  • A to G, chromosome 6 at 22,088,696 bp
  • A to T, chromosome 8 at 70,486,237 bp
  • C to A, chromosome 8 at 84,166,910 bp
  • C to T, chromosome 8 at 107,389,111 bp
  • T to A, chromosome 9 at 62,696,980 bp
  • C to T, chromosome 9 at 113,901,513 bp
  • G to T, chromosome 10 at 100,447,582 bp
  • T to A, chromosome 11 at 103,613,103 bp
  • C to T, chromosome 11 at 104,187,315 bp
  • G to A, chromosome 13 at 113,616,886 bp
  • A to G, chromosome 14 at 99,079,297 bp
  • T to C, chromosome 15 at 101,625,612 bp
  • A to G, chromosome 16 at 10,611,111 bp
  • A to C, chromosome 16 at 38,601,907 bp
  • A to G, chromosome 16 at 59,074,987 bp
  • A to T, chromosome 17 at 67,845,825 bp
  • T to A, chromosome 19 at 57,385,288 bp
  • C to A, chromosome X at 100,933,415 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3011 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040533-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.