Strain Name:
C57BL/6J-MtgxR3015Btlr/Mmmh
Stock Number:
040536-MU
Citation ID:
RRID:MMRRC_040536-MU
Other Names:
R3015 (G1), C57BL/6J-MtgxR3015Btlr
Major Collection:

Gene Information

Suz12
Name: SUZ12 polycomb repressive complex 2 subunit
Synonyms: 2610028O16Rik, D11Ertd530e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52615
Homologene: 32256
Pik3r1
Name: phosphoinositide-3-kinase regulatory subunit 1
Synonyms: p85alpha, p50alpha, p55alpha, PI3K
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18708
HGNC: HGNC:8979
Homologene: 7889
Pax2
Name: paired box 2
Synonyms: Pax-2, Opdc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18504
HGNC: HGNC:8616
Homologene: 2968
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: D630035I23Rik, TRIP8, 5430433L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 108829
Homologene: 3129
Slc2a1
Name: solute carrier family 2 (facilitated glucose transporter), member 1
Synonyms: Glut-1, Glut1, M100200, Rgsc200
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 20525
Homologene: 68520
Golph3l
Name: golgi phosphoprotein 3-like
Synonyms: GPP34R, 2010204I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229593
Homologene: 23082
Ints9
Name: integrator complex subunit 9
Synonyms: D14Ertd231e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 210925
VEGA: 14
Homologene: 10096
4932438A13Rik
Name: RIKEN cDNA 4932438A13 gene
Synonyms: Tweek, FSA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229227
Homologene: 52105
Rnf216
Name: ring finger protein 216
Synonyms: UIP83, F830018F18Rik, 2810055G22Rik, Ubce7ip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108086
Homologene: 19442
Prom1
Name: prominin 1
Synonyms: AC133, CD133, Prom, 4932416E19Rik, Prom-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19126
HGNC: HGNC:9454
Homologene: 4390
Rars
Name: arginyl-tRNA synthetase
Synonyms: 2610037E21Rik, 2610011N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 104458
HGNC: HGNC:9870
Homologene: 68281
Grm7
Name: glutamate receptor, metabotropic 7
Synonyms: mGluR7, Gpr1g, E130018M02Rik, 6330570A01Rik, mGlu7a receptor, SMN2, Tg(SMN2)89Ahmb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108073
HGNC: HGNC:4599
Homologene: 20233
Upf2
Name: UPF2 regulator of nonsense transcripts homolog (yeast)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 326622
Homologene: 6101
Erc2
Name: ELKS/RAB6-interacting/CAST family member 2
Synonyms: D14Ertd171e, ELKS2alpha, CAST
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 238988
Homologene: 69188
Ifrd2
Name: interferon-related developmental regulator 2
Synonyms: SKMc15, 1810034A24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15983
HGNC: HGNC:5457
Homologene: 21341
Nop9
Name: NOP9 nucleolar protein
Synonyms: 2610027L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67842
Homologene: 41678
Rnf115
Name: ring finger protein 115
Synonyms: 2610028E05Rik, Zfp364, Rabring7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67845
Homologene: 69167
Fam207a
Name: family with sequence similarity 207, member A
Synonyms: 1810008A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 108707
VEGA: 10
Homologene: 14384
Ppme1
Name: protein phosphatase methylesterase 1
Synonyms: 1110069N17Rik, 2700017M01Rik, PME-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72590
Homologene: 6099
Katnip
Name: katanin interacting protein
Synonyms: D430042O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233865
Homologene: 45841
Umod
Name: uromodulin
Synonyms: Tamm-Horsfall glycoprotein, uromucoid, urehr4, Urehd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22242
Homologene: 2522
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Rapgef4
Name: Rap guanine nucleotide exchange factor (GEF) 4
Synonyms: 1300003D15Rik, Epac2, cAMP-GEFII, 5730402K07Rik, 6330581N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56508
Homologene: 4451
Tnr
Name: tenascin R
Synonyms: janusin, restrictin, TN-R, J1-tenascin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21960
Homologene: 124416
Aff3
Name: AF4/FMR2 family, member 3
Synonyms: LAF-4, 3222402O04Rik, Laf4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16764
HGNC: HGNC:6473
Homologene: 1718
Scn7a
Name: sodium channel, voltage-gated, type VII, alpha
Synonyms: Nav2.3, NaG, Nav2, 1110034K09Rik, Scn6a, Nax
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20272
Homologene: 55706
Cfap44
Name: cilia and flagella associated protein 44
Synonyms: 6330444M21Rik, D16Ertd642e, Wdr52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 212517
Homologene: 75085
Tspyl3
Name: TSPY-like 3
Synonyms: LOC241732
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241732
Homologene: 19093
Pard3b
Name: par-3 family cell polarity regulator beta
Synonyms: 2810455B10Rik, PAR3beta, PAR3L, PAR3B, 2010002N16Rik, 1810008K04Rik, Als2cr19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72823
Homologene: 35389
Adamts9
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9
Synonyms: 1810011L16Rik, E030027K14Rik, 8430403M15Rik, Mhdaund4, Gsfund3, UND3, Mhdaund3, UND4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101401
Homologene: 18821
Cdh4
Name: cadherin 4
Synonyms: Rcad, R-cadherin, R-Cadh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12561
HGNC: HGNC:1763
Homologene: 48044
Frem3
Name: Fras1 related extracellular matrix protein 3
Synonyms: LOC333315
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 333315
Homologene: 35388
Matn3
Name: matrilin 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 17182
HGNC: HGNC:6909
Homologene: 1785
Ica1
Name: islet cell autoantigen 1
Synonyms: ICA69, 69kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15893
HGNC: HGNC:5343
Homologene: 7777
Vsig10l
Name: V-set and immunoglobulin domain containing 10 like
Synonyms: 2210412E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75690
Homologene: 35386
Fcgbp
Name: Fc fragment of IgG binding protein
Synonyms: A430096B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 215384
Homologene: 68369
Plekha8
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8
Synonyms: FAPP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 231999
Homologene: 32284
Ppp1r26
Name: protein phosphatase 1, regulatory subunit 26
Synonyms: LOC241289
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241289
Homologene: 49400
Shisal2b
Name: shisa like 2B
Synonyms: A930021C24Rik, Fam159b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 77803
VEGA: 13
Homologene: 19019
Dbndd2
Name: dysbindin (dystrobrevin binding protein 1) domain containing 2
Synonyms: 2900022J10Rik, 1110017A21Rik, D2Bwg0891e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 52840
Homologene: 12276
Vash1
Name: vasohibin 1
Synonyms: G630009D10Rik, D930046M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238328
Homologene: 8941
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 38,210,568 bp
  • T to C, chromosome 1 at 62,344,878 bp
  • A to G, chromosome 1 at 159,888,259 bp
  • A to G, chromosome 2 at 5,976,079 bp
  • A to C, chromosome 2 at 28,452,302 bp
  • G to T, chromosome 2 at 66,699,896 bp
  • T to C, chromosome 2 at 72,198,373 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • A to T, chromosome 2 at 153,224,730 bp
  • T to A, chromosome 2 at 164,488,350 bp
  • C to T, chromosome 2 at 179,894,204 bp
  • T to A, chromosome 3 at 36,875,462 bp
  • A to G, chromosome 3 at 95,591,713 bp
  • T to C, chromosome 3 at 96,754,359 bp
  • T to A, chromosome 4 at 119,132,143 bp
  • C to A, chromosome 5 at 44,034,391 bp
  • A to G, chromosome 5 at 143,075,725 bp
  • G to A, chromosome 6 at 8,658,211 bp
  • T to C, chromosome 6 at 54,622,122 bp
  • T to C, chromosome 6 at 92,872,932 bp
  • G to T, chromosome 6 at 110,646,348 bp
  • T to C, chromosome 7 at 28,075,413 bp
  • T to A, chromosome 7 at 43,467,457 bp
  • T to C, chromosome 7 at 100,331,877 bp
  • T to C, chromosome 7 at 119,472,540 bp
  • T to A, chromosome 7 at 125,866,340 bp
  • T to C, chromosome 8 at 80,690,773 bp
  • G to A, chromosome 9 at 107,590,022 bp
  • A to G, chromosome 10 at 67,157,932 bp
  • A to G, chromosome 10 at 77,514,428 bp
  • G to A, chromosome 11 at 35,808,605 bp
  • A to G, chromosome 11 at 80,013,531 bp
  • A to C, chromosome 12 at 8,952,217 bp
  • A to G, chromosome 12 at 86,685,420 bp
  • A to G, chromosome 13 at 101,687,263 bp
  • T to A, chromosome 13 at 104,858,391 bp
  • T to A, chromosome 14 at 28,011,775 bp
  • T to C, chromosome 14 at 55,751,174 bp
  • T to A, chromosome 14 at 64,950,278 bp
  • A to C, chromosome 16 at 44,410,469 bp
  • T to C, chromosome 19 at 44,816,024 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3015 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040536-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.