Strain Name:
C57BL/6J-MtgxR3421Btlr/Mmmh
Stock Number:
040639-MU
Citation ID:
RRID:MMRRC_040639-MU
Other Names:
R3421 (G1), C57BL/6J-MtgxR3421Btlr
Major Collection:

Gene Information

Slco1a5
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Oatp3, Slc21a7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108096
Homologene: 56603
Kifap3
Name: kinesin-associated protein 3
Synonyms: Smg GDS, KAP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16579
Homologene: 7799
Psmb2
Name: proteasome (prosome, macropain) subunit, beta type 2
Synonyms: HC7-I, D4Wsu33e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26445
HGNC: HGNC:9539
Homologene: 2088
Gtf2ird1
Name: general transcription factor II I repeat domain-containing 1
Synonyms: Alb/c-myc line 166.8, ESTM9, c-myc line 166.8, Alb-c-myc line 166.8, WBSCR11, Cream1, GTF3, MusTRD1, binding factor for early enhancer, BEN, Tg(Alb1-Myc)166.8Sst
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 57080
HGNC: HGNC:4661
Homologene: 4158
Slc25a17
Name: solute carrier family 25 (mitochondrial carrier, peroxisomal membrane protein), member 17
Synonyms: 34kDa, PMP34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20524
VEGA: 15
Homologene: 4637
Ric1
Name: RAB6A GEF complex partner 1
Synonyms: C030046E11Rik, C130057E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226089
Homologene: 13806
Sarnp
Name: SAP domain containing ribonucleoprotein
Synonyms: 1110005A23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66118
VEGA: 10
Homologene: 41628
Chrdl2
Name: chordin-like 2
Synonyms: Chl2, 1810022C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69121
Homologene: 128795
Brip1
Name: BRCA1 interacting protein C-terminal helicase 1
Synonyms: 8030460J03Rik, 3110009N10Rik, BACH1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237911
Homologene: 32766
Chst4
Name: carbohydrate sulfotransferase 4
Synonyms: high endothelial cell GlcNAC-6-sulphotransferase, GST-3, HEC-GlcNAc6ST
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26887
HGNC: HGNC:1972
Homologene: 4216
Soat2
Name: sterol O-acyltransferase 2
Synonyms: ACAT2, D15Wsu97e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223920
Homologene: 68355
Col9a2
Name: collagen, type IX, alpha 2
Synonyms: Col9a-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12840
HGNC: HGNC:2218
Homologene: 37535
Acacb
Name: acetyl-Coenzyme A carboxylase beta
Synonyms: Accb, Acc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100705
HGNC: HGNC:85
Homologene: 74382
Atp7b
Name: ATPase, Cu++ transporting, beta polypeptide
Synonyms: Atp7a, Wilson protein, WND
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11979
HGNC: HGNC:870
Homologene: 20063
Agbl3
Name: ATP/GTP binding protein-like 3
Synonyms: Ccp3, Ccp3, 2900053G10Rik, 6530406M24Rik, 4930431N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76223
Homologene: 35330
Ap3b2
Name: adaptor-related protein complex 3, beta 2 subunit
Synonyms: Naptb, beta3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11775
HGNC: HGNC:567
Homologene: 55837
Slc5a4a
Name: solute carrier family 5, member 4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64452
VEGA: 10
Armc4
Name: armadillo repeat containing 4
Synonyms: b2b227.1Clo, b2b643Clo, 4930463I21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 74934
VEGA: 18
Homologene: 9992
Zfp712
Name: zinc finger protein 712
Synonyms: mszf31, mszf89, 4921504N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 78251
VEGA: 13
Homologene: 136296
Zfp217
Name: zinc finger protein 217
Synonyms: 4933431C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228913
Homologene: 4757
Pik3cg
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
Synonyms: PI3Kgamma, p110gamma, PI3Kgamma, PI(3)Kgamma, 5830428L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 30955
HGNC: HGNC:8978
Homologene: 68269
Ccdc40
Name: coiled-coil domain containing 40
Synonyms: B930008I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207607
Homologene: 27890
Igfn1
Name: immunoglobulin-like and fibronectin type III domain containing 1
Synonyms: 9830123M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226438
Homologene: 130054
Kcnip1
Name: Kv channel-interacting protein 1
Synonyms: 2900046L02Rik, KCHIP1, 3202002F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70357
Homologene: 22824
Amn1
Name: antagonist of mitotic exit network 1
Synonyms: C730024G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232566
Homologene: 45811
Zdhhc14
Name: zinc finger, DHHC domain containing 14
Synonyms: B530001K09Rik, New1cp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224454
VEGA: 17
Homologene: 62301
Prex1
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
Synonyms: P-REX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 277360
Homologene: 10821
Kif4-ps
Name: kinesin family member 4, pseudogene
Synonyms: Kif4b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74947
Olfr725
Name: olfactory receptor 725
Synonyms: MOR246-3, MOR246-7_p, GA_x6K02T2PMLR-5725741-5724776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258314
Homologene: 44957
Dtx2
Name: deltex 2, E3 ubiquitin ligase
Synonyms: 2610524D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74198
Homologene: 56904
Olfr1230
Name: olfactory receptor 1230
Synonyms: MOR237-1, GA_x6K02T2Q125-50741934-50741017
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258785
Homologene: 81570
D930048N14Rik
Name: RIKEN cDNA D930048N14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 97775
Mgat4d
Name: MGAT4 family, member C
Synonyms: 4933434I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67555
Homologene: 128538
Nuak2
Name: NUAK family, SNF1-like kinase, 2
Synonyms: Snark, 1200013B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74137
Homologene: 12539
Olfr26
Name: olfactory receptor 26
Synonyms: MTPCR09, GA_x6K02T2PVTD-32550930-32551856, MOR171-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18324
VEGA: 9
HGNC: HGNC:8481
Homologene: 79676
Olfr20
Name: olfactory receptor 20
Synonyms: MOR135-11, Olfr21, MTPCR55, GA_x6K02T2P1NL-3514066-3515010, MTPCR06
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258925
Homologene: 128054
Mr1
Name: major histocompatibility complex, class I-related
Synonyms: H2ls, MR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 15064
HGNC: HGNC:4975
Homologene: 123981
Dmgdh
Name: dimethylglycine dehydrogenase precursor
Synonyms: 1200014D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 74129
Homologene: 8372
Telo2
Name: telomere maintenance 2
Synonyms: 1200003M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 71718
Homologene: 41107
Hoxc6
Name: homeobox C6
Synonyms: Hox-3.3, Hox-6.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 15425
HGNC: HGNC:5128
Homologene: 19533
Gm11787
Name: predicted gene 11787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666513
Saysd1
Name: SAYSVFN motif domain containing 1
Synonyms: 4930488P03Rik, 1810063B07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67509
VEGA: 14
Homologene: 41248
Olfr1288
Name: olfactory receptor 1288
Synonyms: MOR245-9, GA_x6K02T2Q125-72530279-72531217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258395
Homologene: 88360
Tafa1
Name: TAFA chemokine like family member 1
Synonyms: Tafa-1, C630007B19Rik, Fam19a1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320265
Homologene: 45655
Slc7a3
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 3
Synonyms: SLC7A1, SLC7A2, Atrc3, Cat3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11989
Homologene: 56382
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 132,332,080 bp
  • C to T, chromosome 1 at 135,976,917 bp
  • T to C, chromosome 1 at 155,137,591 bp
  • T to A, chromosome 1 at 163,794,026 bp
  • C to G, chromosome 2 at 89,296,553 bp
  • A to G, chromosome 2 at 111,478,952 bp
  • A to G, chromosome 2 at 166,617,854 bp
  • A to G, chromosome 2 at 170,120,017 bp
  • A to G, chromosome 4 at 3,516,370 bp
  • C to G, chromosome 4 at 121,054,258 bp
  • T to C, chromosome 4 at 126,677,837 bp
  • T to A, chromosome 5 at 114,212,636 bp
  • T to A, chromosome 5 at 134,388,500 bp
  • T to A, chromosome 5 at 136,012,478 bp
  • A to G, chromosome 6 at 34,793,965 bp
  • C to A, chromosome 6 at 96,649,138 bp
  • A to T, chromosome 6 at 142,268,238 bp
  • A to T, chromosome 6 at 149,169,452 bp
  • C to T, chromosome 7 at 81,473,850 bp
  • G to A, chromosome 7 at 100,023,868 bp
  • C to T, chromosome 8 at 22,028,670 bp
  • T to C, chromosome 8 at 83,358,143 bp
  • T to C, chromosome 8 at 110,030,406 bp
  • A to G, chromosome 9 at 38,855,325 bp
  • T to C, chromosome 10 at 76,176,573 bp
  • G to A, chromosome 10 at 128,877,326 bp
  • A to G, chromosome 11 at 33,645,594 bp
  • T to C, chromosome 11 at 51,654,958 bp
  • A to G, chromosome 11 at 73,354,634 bp
  • A to T, chromosome 11 at 86,152,669 bp
  • C to T, chromosome 11 at 119,234,779 bp
  • A to T, chromosome 12 at 32,204,739 bp
  • A to T, chromosome 12 at 101,146,971 bp
  • C to T, chromosome 13 at 67,052,392 bp
  • T to C, chromosome 13 at 93,711,361 bp
  • T to A, chromosome 14 at 20,082,926 bp
  • T to C, chromosome 14 at 50,034,540 bp
  • C to T, chromosome 15 at 81,360,700 bp
  • T to C, chromosome 15 at 102,156,809 bp
  • T to A, chromosome 15 at 103,010,895 bp
  • T to A, chromosome 17 at 5,753,091 bp
  • C to T, chromosome 17 at 25,110,752 bp
  • T to C, chromosome 18 at 7,223,523 bp
  • T to C, chromosome 19 at 29,567,590 bp
  • T to A, chromosome X at 101,080,875 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3421 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040639-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.