Strain Name:
C57BL/6J-MtgxR3428Btlr/Mmmh
Stock Number:
040646-MU
Citation ID:
RRID:MMRRC_040646-MU
Other Names:
R3428 (G1), C57BL/6J-MtgxR3428Btlr
Major Collection:

Strain Information

Mtg2
Name: mitochondrial ribosome associated GTPase 2
Synonyms: 1810011P19Rik, D2Bwg0647e, 2900056P18Rik, Gtpbp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 52856
Homologene: 9233
Psma2
Name: proteasome subunit alpha 2
Synonyms: Lmpc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19166
VEGA: 13
HGNC: HGNC:9531
Homologene: 2081
Sec24d
Name: Sec24 related gene family, member D (S. cerevisiae)
Synonyms: 2310020L09Rik, LOC383951
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69608
Homologene: 40986
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, HTRX1, ALL-1, All1, Cxxc7, Mll, Mll1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Bicd1
Name: BICD cargo adaptor 1
Synonyms: B830009D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12121
HGNC: HGNC:1049
Homologene: 37518
Ap1g1
Name: adaptor protein complex AP-1, gamma 1 subunit
Synonyms: gamma-adaptin, Adtg, D8Ertd374e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11765
HGNC: HGNC:555
Homologene: 47995
Prelid1
Name: PRELI domain containing 1
Synonyms: 2610524G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66494
VEGA: 13
Homologene: 40859
Arhgap17
Name: Rho GTPase activating protein 17
Synonyms: WBP15, Nadrin, Rich1, 5730403H17Rik, Nadrin2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70497
Homologene: 9984
Bbs9
Name: Bardet-Biedl syndrome 9 (human)
Synonyms: EST 3159894, E130103I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319845
Homologene: 44480
Setd1a
Name: SET domain containing 1A
Synonyms: KMT2F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233904
Homologene: 52251
Adam18
Name: a disintegrin and metallopeptidase domain 18
Synonyms: Adam27, Dtgn3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 13524
HGNC: HGNC:196
Homologene: 74941
Eng
Name: endoglin
Synonyms: CD105, Endo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13805
HGNC: HGNC:3349
Homologene: 92
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: per, Pcn, Plc, perlecan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Ubr2
Name: ubiquitin protein ligase E3 component n-recognin 2
Synonyms: 9930021A08Rik, E130209G04Rik, ENSMUSG00000043296
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224826
VEGA: 17
Homologene: 26151
Cand2
Name: cullin-associated and neddylation-dissociated 2 (putative)
Synonyms: Tp120b, 2210404G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67088
Homologene: 41657
Nup54
Name: nucleoporin 54
Synonyms: 3110079L04Rik, 54kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269113
Homologene: 41169
Adgrf5
Name: adhesion G protein-coupled receptor F5
Synonyms: 8430401C09Rik, Gpr116
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224792
VEGA: 17
Homologene: 9065
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329178
Homologene: 122243
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Slc20a1
Name: solute carrier family 20, member 1
Synonyms: Glvr-1, Glvr1, PiT-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20515
Homologene: 38049
Slc22a5
Name: solute carrier family 22 (organic cation transporter), member 5
Synonyms: Octn2, Lstpl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20520
Homologene: 68295
Yipf2
Name: Yip1 domain family, member 2
Synonyms: 1300010K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74766
VEGA: 9
Homologene: 23430
Trpv3
Name: transient receptor potential cation channel, subfamily V, member 3
Synonyms: 1110036I10Rik, VRL3, Nh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 246788
Homologene: 17040
Gm5828
Name: predicted gene 5828
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545306
Pfpl
Name: pore forming protein-like
Synonyms: Epcs5, Epcs50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56093
VEGA: 19
Homologene: 130704
Igtp
Name: interferon gamma induced GTPase
Synonyms: Irgm3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16145
Homologene: 23118
CAAA01123846.1
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Or10a5
Name: olfactory receptor family 10 subfamily A member 5
Synonyms: GA_x6K02T2PBJ9-9415724-9416677, MOR263-1, P3, Olfr713
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259036
Homologene: 17470
Adamts14
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 14
Synonyms: TS14, Adamts-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237360
Homologene: 16383
Adrb3
Name: adrenergic receptor, beta 3
Synonyms: beta 3-AR, Beta-3 adrenoceptor, Beta-3 AR, beta3-adrenergic receptor, Adrb-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11556
HGNC: HGNC:288
Homologene: 37250
Tmem181a
Name: transmembrane protein 181A
Synonyms: 5930418K15Rik, Gpr178, C76977, Tmem181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 77106
Homologene: 44787
Alg5
Name: asparagine-linked glycosylation 5 (dolichyl-phosphate beta-glucosyltransferase)
Synonyms: 1500026A19Rik, 2600005J22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66248
Homologene: 7060
Lyzl4
Name: lysozyme-like 4
Synonyms: LYC4, 1810009N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69032
Homologene: 23604
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 16,768,614 bp
  • G to A, chromosome 1 at 66,639,305 bp
  • G to T, chromosome 2 at 32,657,533 bp
  • A to T, chromosome 2 at 129,200,282 bp
  • A to G, chromosome 2 at 180,084,272 bp
  • T to A, chromosome 3 at 54,735,585 bp
  • C to T, chromosome 3 at 123,343,923 bp
  • T to C, chromosome 4 at 137,555,290 bp
  • A to G, chromosome 4 at 156,350,141 bp
  • A to G, chromosome 5 at 92,431,027 bp
  • A to C, chromosome 6 at 115,789,707 bp
  • T to C, chromosome 6 at 149,512,902 bp
  • G to A, chromosome 7 at 107,036,716 bp
  • A to T, chromosome 7 at 123,323,631 bp
  • G to T, chromosome 7 at 127,785,321 bp
  • C to A, chromosome 8 at 24,667,604 bp
  • T to C, chromosome 8 at 27,228,181 bp
  • A to G, chromosome 8 at 109,843,448 bp
  • T to C, chromosome 9 at 21,589,645 bp
  • T to A, chromosome 9 at 22,567,887 bp
  • T to A, chromosome 9 at 44,848,119 bp
  • T to C, chromosome 9 at 121,584,129 bp
  • C to T, chromosome 10 at 61,224,374 bp
  • A to G, chromosome 11 at 53,869,326 bp
  • G to A, chromosome 11 at 58,206,593 bp
  • C to G, chromosome 11 at 59,067,930 bp
  • A to T, chromosome 11 at 73,285,941 bp
  • A to T, chromosome 13 at 14,616,777 bp
  • T to G, chromosome 13 at 55,322,194 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • A to T, chromosome 17 at 43,445,148 bp
  • T to C, chromosome 17 at 46,968,439 bp
  • T to A, chromosome 19 at 12,430,313 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3428 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040646-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.