Strain Name:
C57BL/6J-MtgxR3918Btlr/Mmmh
Stock Number:
040816-MU
Citation ID:
RRID:MMRRC_040816-MU
Other Names:
R3918 (G1), C57BL/6J-MtgxR3918Btlr
Major Collection:

Gene Information

Dysf
Name: dysferlin
Synonyms: 2310004N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 26903
HGNC: HGNC:3097
Homologene: 20748
Egf
Name: epidermal growth factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13645
HGNC: HGNC:3229
Homologene: 1483
Ctnnal1
Name: catenin (cadherin associated protein), alpha-like 1
Synonyms: ACRP, Catnal1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 54366
HGNC: HGNC:2512
Homologene: 2815
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Mras
Name: muscle and microspikes RAS
Synonyms: 2900078C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17532
HGNC: HGNC:7227
Homologene: 7424
Wfs1
Name: wolframin ER transmembrane glycoprotein
Synonyms: Wolfram syndrome 1 homolog (human), wolframin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22393
Homologene: 4380
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12695
Homologene: 72199
Tut7
Name: terminal uridylyl transferase 7
Synonyms: Tent3b, 6030448M23Rik, Zcchc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 214290
VEGA: 13
Homologene: 51941
Pola1
Name: polymerase (DNA directed), alpha 1
Synonyms: Pola
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18968
HGNC: HGNC:9173
Homologene: 6802
Tank
Name: TRAF family member-associated Nf-kappa B activator
Synonyms: E430026L09Rik, I-TRAF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21353
Homologene: 3081
Lef1
Name: lymphoid enhancer binding factor 1
Synonyms: lymphoid enhancer factor 1, Lef-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16842
HGNC: HGNC:6551
Homologene: 7813
Aars
Name: alanyl-tRNA synthetase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234734
HGNC: HGNC:20
Homologene: 1213
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 1110059H15Rik, A130030D10Rik, 4833421P10Rik, Zfp650
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68795
Homologene: 52092
Ak7
Name: adenylate kinase 7
Synonyms: 4930502N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 78801
VEGA: 12
Homologene: 14268
Lpin1
Name: lipin 1
Synonyms: Lipin1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 14245
VEGA: 12
Homologene: 9266
Nsun2
Name: NOL1/NOP2/Sun domain family member 2
Synonyms: Misu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 28114
Homologene: 9817
Ola1
Name: Obg-like ATPase 1
Synonyms: 2510025G09Rik, 2810409H07Rik, 2810405J23Rik, Gtpbp9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67059
Homologene: 5361
Ppip5k2
Name: diphosphoinositol pentakisphosphate kinase 2
Synonyms: Cfap160, Hisppd1, Vip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227399
Homologene: 49409
Tmprss4
Name: transmembrane protease, serine 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214523
Homologene: 80930
Marveld2
Name: MARVEL (membrane-associating) domain containing 2
Synonyms: Tric, Mrvldc2, Tric-c, Tric-b, Tric-a, Tricellulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218518
VEGA: 13
Homologene: 27037
Hmcn1
Name: hemicentin 1
Synonyms: EG545370, LOC240793
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545370
Homologene: 23741
Galnt2
Name: polypeptide N-acetylgalactosaminyltransferase 2
Synonyms: ppGaNTase-T2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108148
HGNC: HGNC:4124
Homologene: 3297
Myh13
Name: myosin, heavy polypeptide 13, skeletal muscle
Synonyms: EO Myosin, MyHC-eo, extraocular myosin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 544791
HGNC: HGNC:7571
Homologene: 55780
Olfr994
Name: olfactory receptor 994
Synonyms: MOR203-4, GA_x6K02T2Q125-46907515-46906571
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258425
Homologene: 17245
Brinp3
Name: bone morphogenetic protein/retinoic acid inducible neural specific 3
Synonyms: Fam5c, B830045N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215378
Homologene: 17786
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Gprc6a
Name: G protein-coupled receptor, family C, group 6, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 210198
VEGA: 10
Homologene: 17529
Adhfe1
Name: alcohol dehydrogenase, iron containing, 1
Synonyms: 6330565B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 76187
Homologene: 5865
Ppp1r36
Name: protein phosphatase 1, regulatory subunit 36
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 210762
VEGA: 12
Homologene: 52094
Mest
Name: mesoderm specific transcript
Synonyms: Peg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 17294
HGNC: HGNC:7028
Homologene: 1800
Ccdc40
Name: coiled-coil domain containing 40
Synonyms: B930008I02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207607
Homologene: 27890
Pcdhb12
Name: protocadherin beta 12
Synonyms: Pcdh3, PcdhbL, Pcdhb5F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93883
Homologene: 87126
Ufsp2
Name: UFM1-specific peptidase 2
Synonyms: 1810047C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 192169
Homologene: 10151
Fbln5
Name: fibulin 5
Synonyms: EVEC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 23876
VEGA: 12
HGNC: HGNC:3602
Homologene: 38170
Trappc13
Name: trafficking protein particle complex 13
Synonyms: 2610524F24Rik, 2410002O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66975
Homologene: 11792
Rnf150
Name: ring finger protein 150
Synonyms: C030044C12Rik, Greul5, A630007N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 330812
Homologene: 33783
Acp2
Name: acid phosphatase 2, lysosomal
Synonyms: LAP, Acp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11432
HGNC: HGNC:123
Homologene: 1217
Best2
Name: bestrophin 2
Synonyms: Vmd2l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 212989
Homologene: 41187
Zfp51
Name: zinc finger protein 51
Synonyms: Zfp-51, zfec12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22709
VEGA: 17
Homologene: 134003
Gm12258
Name: predicted gene 12258
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108167848
Txndc9
Name: thioredoxin domain containing 9
Synonyms: Apacd, ATP binding protein associated with cell differentiation
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98258
Homologene: 4225
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 9,576,216 bp
  • G to A, chromosome 1 at 37,994,050 bp
  • A to G, chromosome 1 at 97,747,656 bp
  • C to A, chromosome 1 at 146,751,861 bp
  • G to C, chromosome 1 at 150,690,610 bp
  • G to T, chromosome 2 at 61,643,786 bp
  • A to T, chromosome 2 at 70,016,130 bp
  • A to G, chromosome 2 at 73,142,339 bp
  • A to T, chromosome 2 at 85,430,730 bp
  • G to T, chromosome 2 at 91,206,006 bp
  • T to C, chromosome 3 at 129,696,860 bp
  • A to G, chromosome 3 at 131,111,641 bp
  • T to C, chromosome 4 at 56,865,000 bp
  • C to A, chromosome 4 at 98,456,218 bp
  • C to A, chromosome 5 at 36,968,624 bp
  • G to A, chromosome 6 at 30,742,750 bp
  • G to A, chromosome 6 at 84,186,509 bp
  • T to A, chromosome 8 at 45,994,101 bp
  • C to T, chromosome 8 at 82,864,461 bp
  • T to G, chromosome 8 at 85,009,724 bp
  • T to C, chromosome 8 at 111,040,142 bp
  • C to T, chromosome 8 at 124,334,262 bp
  • C to T, chromosome 9 at 45,180,666 bp
  • T to A, chromosome 9 at 99,411,420 bp
  • CAAA to CA, chromosome 10 at 51,615,680 bp
  • T to C, chromosome 10 at 77,053,358 bp
  • T to C, chromosome 11 at 58,858,230 bp
  • T to A, chromosome 11 at 65,870,974 bp
  • G to A, chromosome 11 at 67,329,238 bp
  • T to C, chromosome 11 at 119,264,253 bp
  • A to G, chromosome 12 at 16,571,189 bp
  • G to A, chromosome 12 at 76,417,657 bp
  • C to T, chromosome 12 at 101,750,791 bp
  • A to G, chromosome 12 at 105,710,256 bp
  • A to G, chromosome 13 at 59,782,024 bp
  • A to G, chromosome 13 at 69,630,680 bp
  • T to C, chromosome 13 at 100,611,893 bp
  • T to C, chromosome 13 at 104,161,082 bp
  • A to G, chromosome 17 at 21,463,440 bp
  • T to A, chromosome 18 at 37,437,048 bp
  • C to A, chromosome X at 93,461,472 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3918 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040816-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.