Strain Name:
C57BL/6J-MtgxR4344Btlr/Mmmh
Stock Number:
041101-MU
Citation ID:
RRID:MMRRC_041101-MU
Other Names:
R4344 (G1), C57BL/6J-MtgxR4344Btlr
Major Collection:

Gene Information

Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228852
Homologene: 9194
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Rad54l
Name: RAD54 like (S. cerevisiae)
Synonyms: RAD54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19366
HGNC: HGNC:9826
Homologene: 48227
Tmprss6
Name: transmembrane serine protease 6
Synonyms: matriptase-2, 1300008A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 71753
VEGA: 15
Homologene: 12408
Dcc
Name: deleted in colorectal carcinoma
Synonyms: C030036D22Rik, Igdcc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13176
HGNC: HGNC:2701
Homologene: 21081
Fbf1
Name: Fas (TNFRSF6) binding factor 1
Synonyms: 1110033G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217335
Homologene: 16531
Doc2a
Name: double C2, alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13446
HGNC: HGNC:2985
Homologene: 2657
Ccdc187
Name: coiled-coil domain containing 187
Synonyms: 4932418E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329366
Homologene: 77630
Yme1l1
Name: YME1-like 1 (S. cerevisiae)
Synonyms: Ftsh, ATP-dependent metalloprotease FtsH1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27377
Homologene: 31996
Slc38a9
Name: solute carrier family 38, member 9
Synonyms: 9430067K09Rik, 6720411P22Rik, 9130023D20Rik, 4833412L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 268706
VEGA: 13
Homologene: 18139
Fhdc1
Name: FH2 domain containing 1
Synonyms: 6330505N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229474
Homologene: 18920
Cep19
Name: centrosomal protein 19
Synonyms: 1500031L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66994
Homologene: 12065
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dennd6b
Name: DENN/MADD domain containing 6B
Synonyms: 1700027J05Rik, Fam116b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69440
VEGA: 15
Homologene: 57065
Rasef
Name: RAS and EF hand domain containing
Synonyms: RAB45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242505
Homologene: 28424
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Trpm3
Name: transient receptor potential cation channel, subfamily M, member 3
Synonyms: B930001P07Rik, 6330504P12Rik, MLSN2, melastatin 2, LTRPC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226025
VEGA: 19
Homologene: 62287
Adgrb3
Name: adhesion G protein-coupled receptor B3
Synonyms: A830096D10Rik, Bai3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210933
HGNC: HGNC:945
Homologene: 1289
Ces2a
Name: carboxylesterase 2A
Synonyms: 9130231C15Rik, Ces6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102022
HGNC: HGNC:1864
Homologene: 136396
Gm8251
Name: predicted gene 8251
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 102636082
VEGA: 1
Homologene: 141149
Ralgds
Name: ral guanine nucleotide dissociation stimulator
Synonyms: RalGDS, Rgds, Gnds
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19730
HGNC: HGNC:9842
Homologene: 4562
Zmynd15
Name: zinc finger, MYND-type containing 15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 574428
Homologene: 12995
Vmn2r22
Name: vomeronasal 2, receptor 22
Synonyms: EG546913
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 546913
Homologene: 84037
Ccn5
Name: cellular communication network factor 5
Synonyms: rCop1, Crgr4, CCN5, Wisp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22403
Homologene: 2882
Elmo1
Name: engulfment and cell motility 1
Synonyms: CED-12, C230095H21Rik, 6330578D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 140580
Homologene: 56685
Dlgap3
Name: DLG associated protein 3
Synonyms: PSD-95/SAP90-binding protein 3, SAP90/PSD 95 associated protein 3, DAP3, Sapap3, Prpl8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242667
Homologene: 18276
Ugt2b5
Name: UDP glucuronosyltransferase 2 family, polypeptide B5
Synonyms: Udpgt-3, m-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22238
Homologene: 137225
Cd38
Name: CD38 antigen
Synonyms: Cd38-rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12494
HGNC: HGNC:1667
Homologene: 1345
Plod3
Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Synonyms: lysyl hydroxylase 3, LH3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26433
HGNC: HGNC:9083
Homologene: 843
Klk1b7-ps
Name: kallikrein 1-related peptidase b7, pseudogene
Synonyms: mGK-7, Klk-ps2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16604
Sh3bp2
Name: SH3-domain binding protein 2
Synonyms: 3BP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 24055
Homologene: 2276
Ttc39c
Name: tetratricopeptide repeat domain 39C
Synonyms: 1700008N02Rik, 2810439F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72747
Homologene: 124438
Gm5878
Name: predicted gene 5878
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 545861
5031434C07Rik
Name: RIKEN cDNA 5031434C07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100503613
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 25,826,748 bp
  • G to A, chromosome 1 at 44,060,991 bp
  • G to A, chromosome 2 at 23,173,061 bp
  • T to A, chromosome 2 at 26,280,669 bp
  • T to C, chromosome 2 at 28,552,095 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • G to A, chromosome 2 at 158,749,186 bp
  • G to A, chromosome 2 at 163,828,986 bp
  • C to A, chromosome 3 at 84,444,826 bp
  • T to A, chromosome 4 at 73,745,089 bp
  • C to T, chromosome 4 at 116,097,354 bp
  • T to C, chromosome 4 at 127,214,348 bp
  • T to C, chromosome 5 at 34,555,542 bp
  • A to C, chromosome 5 at 43,869,089 bp
  • A to T, chromosome 5 at 87,139,723 bp
  • G to C, chromosome 5 at 136,988,146 bp
  • G to A, chromosome 6 at 85,125,651 bp
  • T to A, chromosome 6 at 112,288,007 bp
  • C to T, chromosome 6 at 123,637,797 bp
  • C to A, chromosome 7 at 43,945,762 bp
  • C to T, chromosome 7 at 126,851,149 bp
  • T to G, chromosome 8 at 104,737,134 bp
  • C to T, chromosome 11 at 70,461,068 bp
  • A to G, chromosome 11 at 116,147,742 bp
  • T to A, chromosome 13 at 13,698,466 bp
  • T to A, chromosome 13 at 20,261,552 bp
  • T to C, chromosome 13 at 112,729,215 bp
  • T to A, chromosome 15 at 78,459,427 bp
  • G to A, chromosome 15 at 89,188,663 bp
  • T to A, chromosome 16 at 15,768,022 bp
  • C to T, chromosome 16 at 32,107,065 bp
  • A to G, chromosome 16 at 32,770,292 bp
  • T to A, chromosome 18 at 12,728,610 bp
  • G to A, chromosome 18 at 71,374,490 bp
  • A to T, chromosome 19 at 22,897,697 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4344 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041101-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.