Strain Name:
C57BL/6J-MtgxR4401Btlr/Mmmh
Stock Number:
041132-MU
Citation ID:
RRID:MMRRC_041132-MU
Other Names:
R4401 (G1), C57BL/6J-MtgxR4401Btlr
Major Collection:

Strain Information

Acvr2a
Name: activin receptor IIA
Synonyms: ActRIIa, tActRII, Acvr2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11480
HGNC: HGNC:173
Homologene: 20391
Hira
Name: histone cell cycle regulator
Synonyms: Tuple1, Gm15797, D16Ertd95e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 15260
HGNC: HGNC:4916
Homologene: 48172
Rps6kc1
Name: ribosomal protein S6 kinase polypeptide 1
Synonyms: B130003F20Rik, RPK118
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 320119
Homologene: 8244
Fnbp4
Name: formin binding protein 4
Synonyms: FBP30
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 55935
Homologene: 9087
Cct2
Name: chaperonin containing TCP1 subunit 2
Synonyms: Cctb
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12461
VEGA: 10
HGNC: HGNC:1615
Homologene: 4696
Ap3b1
Name: adaptor-related protein complex 3, beta 1 subunit
Synonyms: rim2, AP-3, Hps2, recombination induced mutation 2, beta3A
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 11774
VEGA: 13
HGNC: HGNC:566
Homologene: 68125
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100683
Homologene: 39246
Rfng
Name: RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: radical fringe
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19719
HGNC: HGNC:9974
Homologene: 56433
Eif4g1
Name: eukaryotic translation initiation factor 4, gamma 1
Synonyms: E030015G23Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208643
HGNC: HGNC:3296
Homologene: 110725
Eef1d
Name: eukaryotic translation elongation factor 1 delta
Synonyms: 5730529A16Rik, 1700026P12Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66656
HGNC: HGNC:3211
Homologene: 23404
Homer3
Name: homer scaffolding protein 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 26558
Homologene: 37972
Rpf2
Name: ribosome production factor 2 homolog
Synonyms: Bxdc1, 2810470K21Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67239
Homologene: 6404
Atad2b
Name: ATPase family, AAA domain containing 2B
Synonyms: 1110014E10Rik, D530031C13Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320817
VEGA: 12
Homologene: 86351
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: C130041O22Rik, Zfh-4, Zfh4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80892
Homologene: 23477
Slc9b2
Name: solute carrier family 9, subfamily B (NHA2, cation proton antiporter 2), member 2
Synonyms: NHA2, NHE10, C80638, nha-oc, Nhedc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 97086
Homologene: 45381
Slc66a1
Name: solute carrier family 66 member 1
Synonyms: Pqlc2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 212555
Homologene: 56784
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: Heatr7b1, OTTMUSG00000020804, ENSMUSG00000044873
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231470
Homologene: 23516
Srgap1
Name: SLIT-ROBO Rho GTPase activating protein 1
Synonyms: Arhgap13, 4930572H05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 117600
Homologene: 56898
Mthfsd
Name: methenyltetrahydrofolate synthetase domain containing
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234814
Homologene: 11245
Cytip
Name: cytohesin 1 interacting protein
Synonyms: Pscdbp, Cybr, A130053M09Rik, Cbp
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227929
HGNC: HGNC:9506
Homologene: 3167
Cntn4
Name: contactin 4
Synonyms: BIG-2A, Axcam
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 269784
HGNC: HGNC:2174
Homologene: 14257
Cyp2c54
Name: cytochrome P450, family 2, subfamily c, polypeptide 54
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 404195
VEGA: 19
Homologene: 137231
Or4k35
Name: olfactory receptor family 4 subfamily K member 35
Synonyms: Olfr1277, GA_x6K02T2Q125-72321818-72320907, MOR248-11
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258391
Homologene: 105176
Or2a25
Name: olfactory receptor family 2 subfamily A member 25
Synonyms: MOR261-1, GA_x6K02T2P3E9-4647978-4647046, Olfr447
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 258990
Homologene: 17457
Zfp986
Name: zinc finger protein 986
Synonyms: Gm13235
Type: Gene
Species: Mouse
Chromosome: 4
Or51f5
Name: olfactory receptor family 51 subfamily F member 5
Synonyms: GA_x6K02T2PBJ9-5491151-5492095, MOR14-2, Olfr561
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259096
Homologene: 133029
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 620697
Homologene: 115466
Abcb1a
Name: ATP-binding cassette, sub-family B member 1A
Synonyms: MDR3, Pgp, P-glycoprotein, Pgy-3, Evi32, mdr-3, Mdr1a, multiple drug resistant 1a, Pgy3, P-gp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18671
HGNC: HGNC:40
Homologene: 55496
Cage1
Name: cancer antigen 1
Synonyms: CAGE1, Ctag3, 4933427I01Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 71213
Homologene: 18484
Lpcat2
Name: lysophosphatidylcholine acyltransferase 2
Synonyms: lysoPAFAT/LPCAT2, Aytl1, LPCAT2, Aytl1a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 270084
Homologene: 41201
Cracd
Name: capping protein inhibiting regulator of actin
Synonyms: C530008M17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320827
Homologene: 136278
Krtap2-21
Name: keratin associated protein 2-21
Synonyms: Gm11937
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100041488
Homologene: 41961
Gm17613
Name: predicted gene, 17613
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Pla2g1b
Name: phospholipase A2, group IB, pancreas
Synonyms: Pla2a, sPLA2IB
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18778
HGNC: HGNC:9030
Homologene: 715
Gm28379
Name: predicted gene 28379
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Gm6124
Name: predicted gene 6124
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620018
Homologene: 45597
Gm20528
Name: predicted gene 20528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • G to T, chromosome 1 at 88,254,935 bp
  • A to G, chromosome 1 at 190,798,605 bp
  • G to T, chromosome 1 at 190,799,958 bp
  • A to G, chromosome 2 at 48,899,702 bp
  • T to C, chromosome 2 at 58,133,935 bp
  • A to G, chromosome 2 at 90,746,758 bp
  • A to G, chromosome 2 at 111,269,833 bp
  • C to A, chromosome 3 at 5,403,345 bp
  • T to C, chromosome 3 at 135,336,544 bp
  • T to C, chromosome 4 at 139,306,543 bp
  • C to T, chromosome 4 at 145,898,943 bp
  • A to G, chromosome 5 at 8,702,390 bp
  • G to A, chromosome 5 at 76,848,916 bp
  • C to A, chromosome 5 at 96,642,620 bp
  • T to A, chromosome 5 at 115,470,888 bp
  • A to G, chromosome 5 at 144,843,318 bp
  • G to T, chromosome 6 at 42,912,326 bp
  • A to G, chromosome 6 at 106,489,664 bp
  • T to A, chromosome 7 at 39,221,105 bp
  • TCC to TC, chromosome 7 at 85,221,550 bp
  • C to T, chromosome 7 at 102,774,799 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • T to C, chromosome 8 at 92,873,055 bp
  • A to G, chromosome 8 at 121,098,823 bp
  • T to C, chromosome 9 at 66,418,463 bp
  • A to G, chromosome 10 at 40,236,128 bp
  • A to G, chromosome 10 at 117,057,809 bp
  • T to C, chromosome 10 at 121,804,921 bp
  • C to T, chromosome 11 at 99,610,075 bp
  • A to T, chromosome 11 at 120,782,480 bp
  • T to A, chromosome 12 at 4,940,145 bp
  • T to A, chromosome 13 at 38,023,102 bp
  • C to A, chromosome 13 at 94,418,099 bp
  • C to T, chromosome 14 at 120,749,980 bp
  • C to T, chromosome 15 at 75,902,920 bp
  • A to G, chromosome 16 at 18,925,720 bp
  • G to T, chromosome 16 at 20,678,843 bp
  • T to C, chromosome 19 at 40,072,171 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4401 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041132-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.