Strain Name:
C57BL/6J-MtgxR4434Btlr/Mmmh
Stock Number:
041148-MU
Citation ID:
RRID:MMRRC_041148-MU
Other Names:
R4434 (G1), C57BL/6J-MtgxR4434Btlr
Major Collection:

Strain Information

L3mbtl2
Name: L3MBTL2 polycomb repressive complex 1 subunit
Synonyms: 4732493N06Rik, m4mbt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 214669
Homologene: 12882
Fam169a
Name: family with sequence similarity 169, member A
Synonyms: B230112C05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 320557
VEGA: 13
Homologene: 52656
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: 5530401C11Rik, ARHGAP10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Tacc2
Name: transforming, acidic coiled-coil containing protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57752
Homologene: 5087
Nampt
Name: nicotinamide phosphoribosyltransferase
Synonyms: 1110035O14Rik, Visfatin, Pbef1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 59027
VEGA: 12
Homologene: 4201
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: A630086P08Rik, opm, b2b327Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Akap9
Name: A kinase (PRKA) anchor protein (yotiao) 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Cdc37l1
Name: cell division cycle 37-like 1
Synonyms: 2700033A15Rik, Hsp90-associating relative of Cdc37, Harc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 67072
VEGA: 19
Homologene: 9912
Opa1
Name: OPA1, mitochondrial dynamin like GTPase
Synonyms: 1200011N24Rik, lilr3, optic atrophy 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74143
HGNC: HGNC:8140
Homologene: 14618
Tasor
Name: transcription activation suppressor
Synonyms: 4933409E02Rik, D14Abb1e, MommeD6, Fam208a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218850
VEGA: 14
Homologene: 9062
Zfp985
Name: zinc finger protein 985
Synonyms: Gm13154
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 433804
Homologene: 133076
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, Ankyrin-3, AnkG, 2900054D09Rik, Ankyrin-G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Nckap5l
Name: NCK-associated protein 5-like
Synonyms: C230021P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 380969
Homologene: 18924
Scube1
Name: signal peptide, CUB domain, EGF-like 1
Synonyms: A630023E24Rik, 7330410C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 64706
Homologene: 11224
Plxnb2
Name: plexin B2
Synonyms: Debt, 1110007H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 140570
HGNC: HGNC:9104
Homologene: 66630
Tnc
Name: tenascin C
Synonyms: Hxb, TN-C, TN, C130033P17Rik, tenascin-C, cytotactin, hexabrachion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21923
HGNC: HGNC:5318
Homologene: 55636
Clec18a
Name: C-type lectin domain family 18, member A
Synonyms: Mrcl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 353287
Homologene: 18755
Gpn3
Name: GPN-loop GTPase 3
Synonyms: A930018B01Rik, D5Ertd708e, Atpbd1c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68080
Homologene: 6487
Dnaja3
Name: DnaJ heat shock protein family (Hsp40) member A3
Synonyms: Tid-1, 1200003J13Rik, 1810053A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 83945
Homologene: 36170
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74144
Homologene: 10397
Gngt1
Name: guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
Synonyms: G(y)1, Gng1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14699
HGNC: HGNC:4411
Homologene: 7738
Hephl1
Name: hephaestin-like 1
Synonyms: LOC244698, Zp, zyklopen, thd, cw
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244698
Homologene: 9112
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Itgb4
Name: integrin beta 4
Synonyms: CD104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192897
HGNC: HGNC:6158
Homologene: 179
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Gnptab
Name: N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits
Synonyms: EG432486
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432486
Homologene: 32576
Trim65
Name: tripartite motif-containing 65
Synonyms: 4732463G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 338364
Homologene: 27844
Sp7
Name: Sp7 transcription factor 7
Synonyms: Osx, 6430578P22Rik, osterix
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 170574
VEGA: 15
Homologene: 15607
Itgbl1
Name: integrin, beta-like 1
Synonyms: with EGF-like repeat domains, B930011D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 223272
HGNC: HGNC:6164
Homologene: 3519
Hpse2
Name: heparanase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 545291
VEGA: 19
Homologene: 19680
Rgsl1
Name: regulator of G-protein signaling like 1
Synonyms: 4930415K13Rik, Rgsl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240816
Homologene: 129962
Rln1
Name: relaxin 1
Synonyms: rlx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19773
Homologene: 55524
Tmc4
Name: transmembrane channel-like gene family 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 353499
Homologene: 16971
Adam6b
Name: a disintegrin and metallopeptidase domain 6B
Synonyms: 4930523C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238405
Homologene: 128362
Krt77
Name: keratin 77
Synonyms: 4732484G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 406220
Homologene: 45490
Shank3
Name: SH3 and multiple ankyrin repeat domains 3
Synonyms: ProSAP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 58234
Homologene: 75163
Ehbp1l1
Name: EH domain binding protein 1-like 1
Synonyms: G430002G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 114601
VEGA: 19
Homologene: 136059
Zfp109
Name: zinc finger protein 109
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56869
Zswim3
Name: zinc finger SWIM-type containing 3
Synonyms: 4921517A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67538
Homologene: 15436
AI597479
Name: expressed sequence AI597479
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98404
Homologene: 11459
Gcnt4
Name: glucosaminyl (N-acetyl) transferase 4, core 2 (beta-1,6-N-acetylglucosaminyltransferase)
Synonyms: LOC218476, LOC238786, C2GNT3, Gm73
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218476
VEGA: 13
Homologene: 41144
Tex26
Name: testis expressed 26
Synonyms: 4930588N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 75860
Homologene: 51841
Or10j5
Name: olfactory receptor family 10 subfamily J member 5
Synonyms: MOR23, MOR267-13, GA_x6K02T2R7CC-893157-892228, Olfr16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18313
Homologene: 7460
Shc3
Name: src homology 2 domain-containing transforming protein C3
Synonyms: ShcC, N-Shc, Rai
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20418
VEGA: 13
Homologene: 7536
Akr1c19
Name: aldo-keto reductase family 1, member C19
Synonyms: 1810010N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 432720
Homologene: 134145
Prh1
Name: proline rich protein HaeIII subfamily 1
Synonyms: A-type, MP2, Prp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19131
Antxrl
Name: anthrax toxin receptor-like
Synonyms: 1700112N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239029
VEGA: 14
Homologene: 52111
Slbp
Name: stem-loop binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20492
Homologene: 31389
Tacstd2
Name: tumor-associated calcium signal transducer 2
Synonyms: GA733-1, EGP-1, TROP2, Ly97
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56753
Homologene: 1763
Dnah7c
Name: dynein, axonemal, heavy chain 7C
Synonyms: Dnahc7c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100101919
Homologene: 41287
Pax8
Name: paired box 8
Synonyms: Pax-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18510
HGNC: HGNC:8622
Homologene: 2589
Tuba3a
Name: tubulin, alpha 3A
Synonyms: M[a]3, Tuba3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22144
Homologene: 128229
Zfand2b
Name: zinc finger, AN1 type domain 2B
Synonyms: 1110060O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68818
Homologene: 32671
Gm5849
Name: predicted gene 5849
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Golga7
Name: golgi autoantigen, golgin subfamily a, 7
Synonyms: GOLGA3AP1, HSPC041, GCP16, C130038N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 57437
Homologene: 9385
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 43,100,799 bp
  • G to A, chromosome 1 at 46,666,282 bp
  • A to G, chromosome 1 at 75,170,686 bp
  • C to T, chromosome 1 at 153,802,341 bp
  • C to T, chromosome 1 at 172,957,544 bp
  • A to G, chromosome 2 at 20,967,335 bp
  • G to A, chromosome 2 at 24,429,609 bp
  • T to A, chromosome 2 at 164,820,643 bp
  • T to A, chromosome 3 at 90,777,875 bp
  • T to C, chromosome 4 at 64,007,829 bp
  • A to C, chromosome 4 at 147,583,911 bp
  • T to A, chromosome 5 at 4,032,708 bp
  • T to C, chromosome 5 at 33,645,975 bp
  • A to G, chromosome 5 at 122,382,052 bp
  • T to C, chromosome 5 at 149,453,355 bp
  • A to G, chromosome 6 at 3,994,282 bp
  • A to G, chromosome 6 at 67,535,144 bp
  • A to T, chromosome 6 at 125,281,543 bp
  • A to T, chromosome 6 at 132,571,878 bp
  • C to T, chromosome 7 at 3,672,007 bp
  • T to A, chromosome 7 at 24,229,346 bp
  • G to A, chromosome 7 at 130,623,541 bp
  • T to C, chromosome 8 at 23,244,734 bp
  • A to T, chromosome 8 at 111,054,621 bp
  • C to T, chromosome 9 at 15,076,796 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • C to T, chromosome 10 at 69,987,070 bp
  • A to G, chromosome 10 at 88,412,622 bp
  • T to A, chromosome 11 at 66,108,075 bp
  • T to A, chromosome 11 at 115,999,814 bp
  • G to A, chromosome 11 at 116,127,609 bp
  • T to C, chromosome 12 at 32,838,363 bp
  • A to T, chromosome 12 at 51,752,052 bp
  • A to C, chromosome 12 at 113,490,661 bp
  • T to C, chromosome 13 at 4,242,616 bp
  • T to C, chromosome 13 at 51,449,266 bp
  • T to A, chromosome 13 at 96,946,342 bp
  • A to G, chromosome 13 at 97,126,740 bp
  • G to A, chromosome 14 at 27,449,861 bp
  • G to A, chromosome 14 at 34,071,617 bp
  • T to A, chromosome 14 at 103,133,789 bp
  • A to T, chromosome 14 at 123,972,199 bp
  • G to A, chromosome 15 at 47,899,795 bp
  • T to C, chromosome 15 at 81,676,892 bp
  • A to T, chromosome 15 at 83,721,924 bp
  • A to G, chromosome 15 at 89,162,803 bp
  • C to A, chromosome 15 at 89,503,359 bp
  • A to C, chromosome 15 at 99,422,863 bp
  • A to T, chromosome 15 at 101,865,469 bp
  • A to G, chromosome 15 at 102,359,101 bp
  • T to G, chromosome 16 at 4,689,995 bp
  • A to G, chromosome 16 at 29,611,983 bp
  • G to T, chromosome 19 at 5,716,248 bp
  • T to C, chromosome 19 at 29,007,621 bp
  • A to T, chromosome 19 at 29,334,562 bp
  • A to G, chromosome 19 at 43,294,269 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4434 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041148-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.