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Strain Name:
C57BL/6J-MtgxR4434Btlr/Mmmh
Stock Number:
041148-MU
Citation ID:
RRID:MMRRC_041148-MU
Other Names:
R4434 (G1), C57BL/6J-MtgxR4434Btlr
Major Collection:

Strain Information

L3mbtl2
Name: L3MBTL2 polycomb repressive complex 1 subunit
Synonyms: 4732493N06Rik, m4mbt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 214669
Homologene: 12882
Fam169a
Name: family with sequence similarity 169, member A
Synonyms: B230112C05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 320557
VEGA: 13
Homologene: 52656
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: 5530401C11Rik, ARHGAP10
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71435
Homologene: 10822
Tacc2
Name: transforming, acidic coiled-coil containing protein 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57752
Homologene: 5087
Nampt
Name: nicotinamide phosphoribosyltransferase
Synonyms: 1110035O14Rik, Visfatin, Pbef1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 59027
VEGA: 12
Homologene: 4201
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: A630086P08Rik, opm, b2b327Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 43,100,799 bp
  • G to A, chromosome 1 at 46,666,282 bp
  • A to G, chromosome 1 at 75,170,686 bp
  • C to T, chromosome 1 at 153,802,341 bp
  • C to T, chromosome 1 at 172,957,544 bp
  • A to G, chromosome 2 at 20,967,335 bp
  • G to A, chromosome 2 at 24,429,609 bp
  • T to A, chromosome 2 at 164,820,643 bp
  • T to A, chromosome 3 at 90,777,875 bp
  • T to C, chromosome 4 at 64,007,829 bp
  • A to C, chromosome 4 at 147,583,911 bp
  • T to A, chromosome 5 at 4,032,708 bp
  • T to C, chromosome 5 at 33,645,975 bp
  • A to G, chromosome 5 at 122,382,052 bp
  • T to C, chromosome 5 at 149,453,355 bp
  • A to G, chromosome 6 at 3,994,282 bp
  • A to G, chromosome 6 at 67,535,144 bp
  • A to T, chromosome 6 at 125,281,543 bp
  • A to T, chromosome 6 at 132,571,878 bp
  • C to T, chromosome 7 at 3,672,007 bp
  • T to A, chromosome 7 at 24,229,346 bp
  • G to A, chromosome 7 at 130,623,541 bp
  • T to C, chromosome 8 at 23,244,734 bp
  • A to T, chromosome 8 at 111,054,621 bp
  • C to T, chromosome 9 at 15,076,796 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • C to T, chromosome 10 at 69,987,070 bp
  • A to G, chromosome 10 at 88,412,622 bp
  • T to A, chromosome 11 at 66,108,075 bp
  • T to A, chromosome 11 at 115,999,814 bp
  • G to A, chromosome 11 at 116,127,609 bp
  • T to C, chromosome 12 at 32,838,363 bp
  • A to T, chromosome 12 at 51,752,052 bp
  • A to C, chromosome 12 at 113,490,661 bp
  • T to C, chromosome 13 at 4,242,616 bp
  • T to C, chromosome 13 at 51,449,266 bp
  • T to A, chromosome 13 at 96,946,342 bp
  • A to G, chromosome 13 at 97,126,740 bp
  • G to A, chromosome 14 at 27,449,861 bp
  • G to A, chromosome 14 at 34,071,617 bp
  • T to A, chromosome 14 at 103,133,789 bp
  • A to T, chromosome 14 at 123,972,199 bp
  • G to A, chromosome 15 at 47,899,795 bp
  • T to C, chromosome 15 at 81,676,892 bp
  • A to T, chromosome 15 at 83,721,924 bp
  • A to G, chromosome 15 at 89,162,803 bp
  • C to A, chromosome 15 at 89,503,359 bp
  • A to C, chromosome 15 at 99,422,863 bp
  • A to T, chromosome 15 at 101,865,469 bp
  • A to G, chromosome 15 at 102,359,101 bp
  • T to G, chromosome 16 at 4,689,995 bp
  • A to G, chromosome 16 at 29,611,983 bp
  • G to T, chromosome 19 at 5,716,248 bp
  • T to C, chromosome 19 at 29,007,621 bp
  • A to T, chromosome 19 at 29,334,562 bp
  • A to G, chromosome 19 at 43,294,269 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4434 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041148-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.