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Strain Detail Sheet

Strain Name:
STOCK Id2tm1Mais/Mmjax
Stock Number:
041568-JAX
Citation ID:
RRID:MMRRC_041568-JAX
Other Names:
Id2 null, Id2-/-, Id2 null, Id2 knockout

Strain Information

Gene Details

Id2tm1Mais
Name: inhibitor of DNA binding 2; targeted mutation 1, Mark A Israel
Synonyms: Id2tm1Jdu
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Id2
Name: inhibitor of DNA binding 2
Synonyms: inhibitor of differentiation 2, C78922, Idb2, bHLHb26
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knockout
NCBI: 15902
VEGA: 12
HGNC: HGNC:5361
Homologene: 1632

Additional Resources
IMPC
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IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
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Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A 1.9 kb portion of the Id2 gene, including 560 bp 5’ upstream of the transcription start site (containing a significant proportion of the promoter region) and exons 1 and 2, was replaced by a 1.8 kb neomycin resistance cassette (Neo). This strain may be useful for studying ID2 as a regulator of circadian rhythm and metabolic homeostasis.
Genotype Determination
ES Cell Line
JM-1

Strain Description

Phenotype
ID2 (inhibitor of DNA binding 2) is a transcriptional regulator containing a helix-loop-helix domain. ID genes are involved in multiple systems including circadian rhythm biology, glucose and lipid metabolism, immune cell function, organ development and tumorigenesis. The Id2 knockout allele has a PGK-neo cassette replacing exons 1-2 of the gene. Mice homozygous for this null allele exhibit multiple alterations in the circadian system including: disrupted circadian rhythms (25% of mice), rapid entrainment following alterations in photoschedule and an increase in light-induced phase shifts. Some mice demonstrate circling behavior and jumpiness, although overall locomotor activity is reduced. Males exhibit increased glucose tolerance, increased glucose uptake in skeletal muscle and improved insulin sensitivity. Males are smaller than littermates, and lean, although they consume more food than females. Females are also smaller, but the difference is less pronounced. Adipogenesis is impaired, resulting in reduced fat deposits and lipid storage in white adipose tissue and liver, respectively. The donating investigator reports that on the C57BL/6 background few homozygotes survive (less than 1%). The strain is maintained on a mixed 129X1, C57BL/6, FVB/N background, on this background homozygous survivorship is increased to 10%. Survivorship is increased under germ-free conditions, suggesting that mice are immunocompromised. This strain may be useful for studying ID2 as a regulator of circadian rhythm and metabolic homeostasis.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • ARNTL Transcription Factors
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Biological Clocks/physiology
  • CLOCK Proteins
  • Circadian Rhythm/physiology
  • Gene Expression Regulation
  • Inhibitor of Differentiation Protein 2/genetics
  • Inhibitor of Differentiation Protein 2/metabolism
  • Inhibitor of Differentiation Proteins/genetics
  • Inhibitor of Differentiation Proteins/metabolism
  • Mice
  • Mice, Knockout
  • Motor Activity/physiology
  • Myocardium/metabolism
  • Photoperiod
  • Promoter Regions, Genetic
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Suprachiasmatic Nucleus/metabolism
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • Blotting, Western
  • Female
  • Gene Expression Profiling
  • Inhibitor of Differentiation Protein 2/physiology
  • Liver/metabolism
  • Male
  • Nuclear Proteins/genetics
  • Nuclear Proteins/metabolism
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Repressor Proteins/physiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Adult Stem Cells/physiology
  • Animals, Newborn
  • Astrocytes/physiology
  • Bromodeoxyuridine/metabolism
  • Cell Count/methods
  • Cell Differentiation/genetics
  • Cells, Cultured
  • Discrimination (Psychology)/physiology
  • Dopamine/metabolism
  • Gene Expression Regulation, Developmental/genetics
  • Glial Fibrillary Acidic Protein/metabolism
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Inhibitor of Differentiation Protein 2/deficiency
  • Neurogenesis/genetics
  • Neurogenesis/physiology
  • Neurons/physiology
  • Olfactory Bulb/cytology
  • Olfactory Bulb/growth & development
  • Olfactory Pathways/cytology
  • Olfactory Pathways/physiology
  • Smell/genetics
  • Statistics, Nonparametric
  • Transcription Factor HES-1
  • Tyrosine 3-Monooxygenase/metabolism
  • ARNTL Transcription Factors/metabolism
  • CLOCK Proteins/metabolism
  • Cytoplasm/metabolism
  • Fibroblasts/cytology
  • Fibroblasts/metabolism
  • Humans
  • Mice, Inbred C57BL
  • NIH 3T3 Cells
  • Period Circadian Proteins/metabolism
  • Two-Hybrid System Techniques
  • Adipocytes/pathology
  • Adipocytes, White/pathology
  • Adipose Tissue, Brown/metabolism
  • Adipose Tissue, Brown/pathology
  • Aging/metabolism
  • Aging/pathology
  • Aging/physiology
  • Biological Transport/genetics
  • Biological Transport/physiology
  • Body Weight/genetics
  • Body Weight/physiology
  • Cell Size
  • Circadian Rhythm
  • Diglycerides/metabolism
  • Eating/genetics
  • Eating/physiology
  • Feeding Behavior/physiology
  • Gene Deletion
  • Glucose/metabolism
  • Glucose Tolerance Test
  • Homeostasis/genetics
  • Homeostasis/physiology
  • Insulin Resistance/genetics
  • Insulin Resistance/physiology
  • Motor Activity/genetics
  • Muscle, Skeletal/metabolism
  • Muscle, Skeletal/pathology
  • Sex Characteristics
  • Adipose Tissue, White/metabolism
  • Blood Glucose/metabolism
  • Diet, High-Fat
  • Dietary Fats/administration & dosage
  • Fatty Liver/etiology
  • Homeostasis/drug effects
  • Lipid Metabolism
  • Phenotype
  • Absorptiometry, Photon
  • Adipose Tissue/metabolism
  • Adipose Tissue/pathology
  • Body Composition
  • Magnetic Resonance Imaging
  • Obesity/diagnosis
  • Obesity/metabolism
  • Obesity/physiopathology
  • Tomography, X-Ray Computed
Strain Development
A targeting vector containing the neomycin resistance cassette was used to replace a region 560 bp 5’ upstream of the transcription start site and exons 1 and 2 using homologous recombination. Exons 1-2 encode the entire coding domain. The construct was electroporated into 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and then crossed to FVB/N mice. The donating investigator maintains the strain on a mixed 129X1/C57BL/6/FVB background. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Hematology
  • Immunology and Inflammation
  • Metabolism
  • Neurobiology
  • Obesity
  • Reproduction

Strain Origin

Donor
Giles Duffield, Ph.D., University of Notre Dame.
Primary Reference

Duffield GE, Robles-Murguia M, Watson NP, Mantani A, Peirson SN, Loros JJ, Israel MA, Dunlap JC. A role for the Id2 gene in regulating photic entrainment of the mammalian circadian system. Curr Biol. 2009 Feb 24;19(4):297-304. Epub 2009 Feb 12. (Medline PMID: 19217292)

Hou TY, Ward SM, Murad JM, Watson NP, Israel MA, and Duffield GE. Inhibitor of DNA binding 2 (ID2) is a rhythmically expressed transcriptional repressor required for circadian clock output in the mouse liver. J Biol Chem. 2009 Nov 13;284(46):31735-45. Epub 2009 Sep 9. (Medline PMID: 19740747)

Havrda MC, Harris BT, Mantani A, Ward NM, Paolella BR, Cuzon VC, Yeh HH, Israel MA. Id2 is required for specification of dopaminergic neurons during adult olfactory neurogenesis. J Neurosci. 2008 Dec 24;28(52):14074-86. (Medline PMID: 19109490)

Ward SM, Fernando S, Hou TY, Duffield GE. 2010. The transcriptional repressor ID2 can interact with the canonical clock components CLOCK and BMAL1 and mediate inhibitory effects on mPer1 expression. J Biol Chem. 2010 Dec 10;285(50):38987-9000. Epub 2010 Sep 22. (Medline PMID: 20861012)

Mathew D, Zhou P, Pywell CM, Van der Veen DR, Shao J, Xi Y, Bonar NA, Hummel A, Chapman S, Leevy WM, Duffield GE. 2013. Ablation of the Id2 gene results in altered circadian feeding behavior, and sex-specific enhancement of insulin sensitivity and elevated glucose uptake in skeletal muscle and brown adipose tissue. PLoS One. 2013 Sep 2;8(9):e73064. (Medline PMID: 24023810)

Zhou P, Hummel AD, Pywell CM, Dong XC, Duffield GE. 2014. High fat diet rescues disturbances to metabolic homeostasis and survival in the Id2 null mouse in a sex-specific manner. Biochem Biophys Res Commun. 2014 Aug 29;451(3):374-81. Epub 2014 Aug 7.(Medline PMID: 25108156)

Metzinger M, Miramontes B, Zhou P, Liu Y, Peng J, Chapman S, Sun L, Sasser TA, Duffield GE, Stack MS, Leevy WM. 2014. Correlation of X-ray Computed Tomography with Quantitative Nuclear Magnetic Resonance (QMR) Methods for Pre-Clinical Measurement of Adipose and Lean Tissues in Living Mice. Sensors (Basel). 2014 Oct 8;14(10):18526-42. (Medline PMID: 25299952)

Colony and Husbandry Information

Special Considerations

Comments by donor: high fat diet/love mash for heterozygote breeding females and for weaned animals improves survivorship of homozygote knockouts.

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
mixed

Breeding

MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Heterozygous female x Heterozygous male littermate
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
041568-JAX-EMBRYO Cryo-preserved embryos $1,090.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
041568-JAX-RESUS Litter recovered from cryo-archive $2,123.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.