Strain Name:
C57BL/6J-MtgxR4522Btlr/Mmmh
Stock Number:
041765-MU
Citation ID:
RRID:MMRRC_041765-MU
Other Names:
R4522 (G1), C57BL/6J-MtgxR4522Btlr
Major Collection:

Gene Information

Ccdc88c
Name: coiled-coil domain containing 88C
Synonyms: Daple, 0610010D24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68339
VEGA: 12
Homologene: 18903
Pcbp1
Name: poly(rC) binding protein 1
Synonyms: [a]CP-1, WBP17, hnRNP E1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 23983
HGNC: HGNC:8647
Homologene: 68506
Mark2
Name: MAP/microtubule affinity regulating kinase 2
Synonyms: Par-1, Emk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13728
HGNC: HGNC:3332
Homologene: 69013
Proser1
Name: proline and serine rich 1
Synonyms: 2810046L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 212127
Homologene: 13463
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
R3hdm1
Name: R3H domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226412
HGNC: HGNC:9757
Homologene: 9108
Xdh
Name: xanthine dehydrogenase
Synonyms: Xox-1, Xox1, Xor, xanthine oxidase, XO
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22436
VEGA: 17
Homologene: 324
Sec23b
Name: SEC23 homolog B, COPII coat complex component
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27054
Homologene: 74571
Dock7
Name: dedicator of cytokinesis 7
Synonyms: 3110056M06Rik, LOC242555, m
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67299
Homologene: 23566
Tubb4b
Name: tubulin, beta 4B class IVB
Synonyms: 4930542G03Rik, Tubb2c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227613
Homologene: 68504
Ctsa
Name: cathepsin A
Synonyms: PPCA, Ppgb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19025
HGNC: HGNC:9251
Homologene: 80163
Nop2
Name: NOP2 nucleolar protein
Synonyms: 120kDa, Nol1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 110109
HGNC: HGNC:7867
Homologene: 135865
Zfp105
Name: zinc finger protein 105
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22646
Homologene: 2568
Babam2
Name: BRISC and BRCA1 A complex member 2
Synonyms: 6030405P19Rik, B830038C02Rik, Bre
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 107976
HGNC: HGNC:1106
Homologene: 3604
Brip1
Name: BRCA1 interacting protein C-terminal helicase 1
Synonyms: BACH1, 8030460J03Rik, 3110009N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237911
Homologene: 32766
Rpl10l
Name: ribosomal protein L10-like
Synonyms: EG238217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238217
Homologene: 68830
Ush2a
Name: usherin
Synonyms: MUSH2A, A930011D15Rik, LOC269160, LOC381317, A930037M10Rik, Ushrn, Ush2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22283
Homologene: 66151
Zfp385b
Name: zinc finger protein 385B
Synonyms: B830010L13Rik, C130013B13Rik, Zfp533
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241494
Homologene: 17606
Nwd1
Name: NACHT and WD repeat domain containing 1
Synonyms: A230063L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319555
Homologene: 72261
Plce1
Name: phospholipase C, epsilon 1
Synonyms: 4933403A21Rik, PLCepsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74055
Homologene: 9478
Cacna1b
Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms: Cchn1a, alpha(1B), Cav2.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12287
HGNC: HGNC:1389
Homologene: 20184
Ubqlnl
Name: ubiquilin-like
Synonyms: LOC244179, 4922504M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244179
Homologene: 17034
Vmn2r72
Name: vomeronasal 2, receptor 72
Synonyms: EG244114, Vmn2r72-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244114
Homologene: 115466
Chaf1b
Name: chromatin assembly factor 1, subunit B (p60)
Synonyms: 2600017H24Rik, MPHOSPH7, CAF1, CAF-I 60 kDa subunit, CAF-1 subunit B, CAF-IP60, CAF1P60, CAF1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 110749
HGNC: HGNC:1911
Homologene: 48346
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Speg
Name: SPEG complex locus
Synonyms: BPEG, SPEGbeta, SPEGalpha, D1Bwg1450e, SPEG, Apeg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11790
Homologene: 55619
Fbxo41
Name: F-box protein 41
Synonyms: D6Ertd538e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330369
Homologene: 19837
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 209683
Homologene: 41023
Tmod2
Name: tropomodulin 2
Synonyms: NTMOD, N-Tmod, neural tropomodulin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 50876
VEGA: 9
Homologene: 22817
Tmem38a
Name: transmembrane protein 38A
Synonyms: 1110001E17Rik, TRIC-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74166
Homologene: 11449
Ptpn18
Name: protein tyrosine phosphatase, non-receptor type 18
Synonyms: HSCF, FLP1, Ptpk1, PTP-K1, PTP-HSCF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19253
HGNC: HGNC:9649
Homologene: 74971
Ddah2
Name: dimethylarginine dimethylaminohydrolase 2
Synonyms: Clone 7u, Ddah, NG30, G6a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 51793
HGNC: HGNC:2716
Homologene: 8510
Olfr1132
Name: olfactory receptor 1132
Synonyms: GA_x6K02T2Q125-49140947-49140021, MOR177-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258833
Homologene: 121518
Gm7173
Name: predicted gene 7173
Synonyms:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
1600012P17Rik
Name: RIKEN cDNA 1600012P17 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Fam122c
Name: family with sequence similarity 122, member C
Synonyms: 4930527G05Rik, 4930432H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 73866
Homologene: 87062
Gm3739
Name: predicted gene 3739
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100042235
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,472,960 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • A to G, chromosome 1 at 75,428,330 bp
  • T to C, chromosome 1 at 128,193,769 bp
  • A to T, chromosome 1 at 158,969,797 bp
  • C to T, chromosome 1 at 188,864,625 bp
  • C to T, chromosome 2 at 24,654,430 bp
  • A to G, chromosome 2 at 25,224,079 bp
  • T to C, chromosome 2 at 77,476,780 bp
  • T to A, chromosome 2 at 87,635,151 bp
  • T to A, chromosome 2 at 144,578,366 bp
  • T to C, chromosome 2 at 164,834,810 bp
  • A to G, chromosome 3 at 53,467,125 bp
  • T to C, chromosome 4 at 98,962,224 bp
  • G to A, chromosome 5 at 32,007,242 bp
  • A to G, chromosome 5 at 111,280,172 bp
  • A to G, chromosome 6 at 85,484,042 bp
  • T to C, chromosome 6 at 86,525,050 bp
  • G to T, chromosome 6 at 125,133,552 bp
  • T to A, chromosome 7 at 85,751,926 bp
  • C to T, chromosome 7 at 104,149,718 bp
  • C to T, chromosome 8 at 72,572,161 bp
  • A to T, chromosome 8 at 72,670,951 bp
  • T to C, chromosome 9 at 75,592,584 bp
  • T to A, chromosome 9 at 122,930,056 bp
  • A to G, chromosome 10 at 50,660,670 bp
  • T to C, chromosome 11 at 86,189,801 bp
  • T to C, chromosome 12 at 66,283,738 bp
  • G to T, chromosome 12 at 100,913,332 bp
  • T to A, chromosome 14 at 7,299,398 bp
  • G to A, chromosome 16 at 93,901,295 bp
  • T to A, chromosome 17 at 35,060,933 bp
  • C to A, chromosome 17 at 73,898,344 bp
  • A to T, chromosome 19 at 7,285,948 bp
  • T to C, chromosome 19 at 38,524,319 bp
  • G to A, chromosome X at 53,293,499 bp
  • A to T, chromosome X at 79,509,995 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4522 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041765-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.