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Strain Name:
C57BL/6J-MtgxR4559Btlr/Mmmh
Stock Number:
041785-MU
Citation ID:
RRID:MMRRC_041785-MU
Other Names:
R4559 (G1), C57BL/6J-MtgxR4559Btlr
Major Collection:

Strain Information

Sf1
Name: splicing factor 1
Synonyms: WBP4, MZFM, CW17R, Zfp162
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22668
Homologene: 134065
Prkacb
Name: protein kinase, cAMP dependent, catalytic, beta
Synonyms: cAMP-dependent protein kinase C beta, Pkacb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18749
HGNC: HGNC:9381
Homologene: 121718
Slc6a12
Name: solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Synonyms: Gabt2, BGT1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14411
Homologene: 128225
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Grin3a
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: NMDAR-L, NR3A, A830097C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242443
Homologene: 128613
Dennd2b
Name: DENN domain containing 2B
Synonyms: 2610305K15Rik, 2010004M01Rik, St5, Denn2b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76954
Homologene: 3951
Hdac5
Name: histone deacetylase 5
Synonyms: mHDA1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15184
Homologene: 3995
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 17,004,511 bp
  • G to A, chromosome 1 at 54,353,013 bp
  • T to C, chromosome 1 at 57,382,924 bp
  • G to A, chromosome 1 at 131,872,567 bp
  • G to A, chromosome 2 at 76,919,546 bp
  • C to A, chromosome 2 at 89,609,699 bp
  • T to A, chromosome 2 at 125,351,714 bp
  • A to G, chromosome 2 at 150,638,485 bp
  • T to A, chromosome 2 at 163,472,673 bp
  • A to G, chromosome 2 at 173,661,433 bp
  • A to G, chromosome 3 at 40,513,091 bp
  • C to T, chromosome 3 at 53,654,321 bp
  • T to C, chromosome 3 at 59,007,102 bp
  • A to G, chromosome 3 at 146,745,392 bp
  • T to C, chromosome 4 at 49,844,555 bp
  • G to T, chromosome 4 at 96,112,957 bp
  • C to A, chromosome 4 at 128,615,770 bp
  • T to A, chromosome 5 at 32,332,831 bp
  • A to G, chromosome 5 at 96,781,289 bp
  • C to A, chromosome 5 at 120,916,895 bp
  • T to C, chromosome 6 at 72,615,681 bp
  • A to G, chromosome 6 at 85,910,585 bp
  • G to A, chromosome 6 at 121,363,861 bp
  • A to G, chromosome 6 at 125,360,766 bp
  • A to T, chromosome 7 at 29,332,253 bp
  • A to G, chromosome 7 at 100,998,156 bp
  • G to A, chromosome 7 at 103,979,560 bp
  • A to G, chromosome 7 at 105,405,685 bp
  • A to G, chromosome 7 at 109,525,578 bp
  • A to G, chromosome 7 at 112,955,131 bp
  • A to G, chromosome 8 at 11,562,090 bp
  • T to C, chromosome 8 at 13,598,259 bp
  • T to A, chromosome 8 at 67,960,647 bp
  • A to G, chromosome 8 at 125,671,814 bp
  • A to T, chromosome 9 at 3,018,945 bp
  • A to G, chromosome 10 at 19,626,712 bp
  • A to G, chromosome 10 at 19,749,284 bp
  • G to T, chromosome 10 at 77,988,883 bp
  • A to G, chromosome 11 at 83,004,744 bp
  • T to C, chromosome 11 at 102,199,102 bp
  • A to G, chromosome 12 at 4,943,223 bp
  • A to T, chromosome 13 at 23,878,712 bp
  • G to T, chromosome 14 at 64,806,132 bp
  • T to C, chromosome 14 at 121,272,801 bp
  • A to G, chromosome 15 at 85,438,120 bp
  • C to A, chromosome 17 at 25,090,767 bp
  • A to T, chromosome 17 at 33,538,375 bp
  • A to T, chromosome 17 at 66,683,408 bp
  • T to C, chromosome 18 at 14,813,526 bp
  • T to A, chromosome 18 at 20,470,921 bp
  • C to T, chromosome 18 at 58,076,074 bp
  • C to T, chromosome 18 at 67,360,228 bp
  • T to A, chromosome 18 at 67,871,513 bp
  • GGCAGCAGCAGCAGCAGCAGC to GGCAGCAGCAGCAGCAGC, chromosome 19 at 6,374,815 bp
  • G to A, chromosome 19 at 24,030,549 bp
  • G to A, chromosome X at 53,260,677 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4559 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041785-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.