Strain Name:
C57BL/6J-MtgxR4565Btlr/Mmmh
Stock Number:
041790-MU
Citation ID:
RRID:MMRRC_041790-MU
Other Names:
R4565 (G1), C57BL/6J-MtgxR4565Btlr
Major Collection:

Gene Information

Cnst
Name: consortin, connexin sorting protein
Synonyms: 9630058J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 226744
Homologene: 17139
Dhx35
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 35
Synonyms: 1200009D07Rik, Ddx35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 71715
Homologene: 6406
Gtse1
Name: G two S phase expressed protein 1
Synonyms: Gtse-1, B99
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 29870
VEGA: 15
Homologene: 8489
Rab11fip3
Name: RAB11 family interacting protein 3 (class II)
Synonyms: Rab11-FIP3, D030060O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 215445
Homologene: 49396
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D7H15F32S1, jdf2, D7H15F37S1, rjs, D15F32S1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Bptf
Name: bromodomain PHD finger transcription factor
Synonyms: 9430093H17Rik, Falz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 207165
HGNC: HGNC:3581
Homologene: 114397
Anxa2
Name: annexin A2
Synonyms: annexin II, lipocortin II, 36-kDa calelectrin, Cal1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12306
HGNC: HGNC:537
Homologene: 20857
Rbmxl1
Name: RNA binding motif protein, X-linked like-1
Synonyms: Hnrpg, hnRNP G, Rbmxrt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 19656
Homologene: 84779
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, P1-Loop, Dnahc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Limk2
Name: LIM motif-containing protein kinase 2
Synonyms: A930024P04Rik, LIM kinase 2, whe, Limk2b, Limk2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 16886
HGNC: HGNC:6614
Homologene: 55911
Afg3l1
Name: AFG3-like AAA ATPase 1
Synonyms: 3110061K15Rik, 1700047G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 114896
HGNC: HGNC:314
Homologene: 134198
Pfdn5
Name: prefoldin 5
Synonyms: EIG-1, MM-1, c-myc binding protein MM-1, 1700010A06Rik, D15Ertd697e, 1190001O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 56612
HGNC: HGNC:8869
Homologene: 1972
Ttc13
Name: tetratricopeptide repeat domain 13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234875
Homologene: 11569
Rasgef1b
Name: RasGEF domain family, member 1B
Synonyms: Gpig4, 4732452O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 320292
Homologene: 14860
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77505
Homologene: 131117
Trpm3
Name: transient receptor potential cation channel, subfamily M, member 3
Synonyms: LTRPC3, 6330504P12Rik, MLSN2, melastatin 2, B930001P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226025
VEGA: 19
Homologene: 62287
Trpm6
Name: transient receptor potential cation channel, subfamily M, member 6
Synonyms: CHAK2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 225997
VEGA: 19
Homologene: 9767
Haspin
Name: histone H3 associated protein kinase
Synonyms: Gsg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 14841
Homologene: 49236
Oas3
Name: 2'-5' oligoadenylate synthetase 3
Synonyms: Oasl10, 2'-5' oligoadenylate synthetase-like 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 246727
HGNC: HGNC:8088
Homologene: 4510
Dpy19l1
Name: dpy-19-like 1 (C. elegans)
Synonyms: 1100001I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 244745
Homologene: 14762
Dcdc2b
Name: doublecortin domain containing 2b
Synonyms: Gm12964, LOC384062
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100504491
Homologene: 82651
Olfr447
Name: olfactory receptor 447
Synonyms: MOR261-1, GA_x6K02T2P3E9-4647978-4647046
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 258990
Homologene: 17457
Slc9b1
Name: solute carrier family 9, subfamily B (NHA1, cation proton antiporter 1), member 1
Synonyms: 4933425K02Rik, 1700094G20Rik, Nhedc1, 4933424B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 74446
Homologene: 49914
Acot11
Name: acyl-CoA thioesterase 11
Synonyms: Them1, BFIT1, 2010309H15Rik, Thea, 1110020M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329910
Homologene: 11977
Zfp618
Name: zinc finger protein 618
Synonyms: D430033D05Rik, 2810040O04Rik, 2810031P15Rik, Nedd10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 72701
Homologene: 18975
Pcdhb17
Name: protocadherin beta 17
Synonyms: Pcdhb16, PcdhbQ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93888
Homologene: 81881
Sp110
Name: Sp110 nuclear body protein
Synonyms: Ifi75, Ipr1, 5031415C07Rik, 5830484A20Rik, 52kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 109032
HGNC: HGNC:5401
Homologene: 82192
Jph1
Name: junctophilin 1
Synonyms: JP-1, mitsugumin72, ENSMUSG00000054314
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 57339
Homologene: 10761
Alkbh1
Name: alkB homolog 1, histone H2A dioxygenase
Synonyms: Nrp, alkB, Alkbh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 211064
Homologene: 4393
Olfr19
Name: olfactory receptor 19
Synonyms: MTPCR15, GA_x54KRFPKG5P-13123979-13123050, M12, MOR140-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 18316
HGNC: HGNC:8363
Homologene: 137402
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 17,004,202 bp
  • C to G, chromosome 1 at 85,589,118 bp
  • A to G, chromosome 1 at 179,604,549 bp
  • C to A, chromosome 2 at 158,849,535 bp
  • G to T, chromosome 3 at 135,382,717 bp
  • T to C, chromosome 4 at 63,121,351 bp
  • C to T, chromosome 4 at 106,760,130 bp
  • T to C, chromosome 4 at 129,610,985 bp
  • T to C, chromosome 5 at 99,227,947 bp
  • A to G, chromosome 5 at 120,771,039 bp
  • A to T, chromosome 6 at 42,911,538 bp
  • T to C, chromosome 7 at 56,153,838 bp
  • T to A, chromosome 7 at 105,651,956 bp
  • G to A, chromosome 8 at 78,506,010 bp
  • A to T, chromosome 8 at 123,501,869 bp
  • A to T, chromosome 8 at 124,682,087 bp
  • A to T, chromosome 9 at 24,432,388 bp
  • A to T, chromosome 9 at 69,489,737 bp
  • A to G, chromosome 11 at 3,348,634 bp
  • G to A, chromosome 11 at 73,137,619 bp
  • G to A, chromosome 11 at 107,073,010 bp
  • T to C, chromosome 12 at 87,431,466 bp
  • T to G, chromosome 15 at 85,875,184 bp
  • T to C, chromosome 15 at 102,326,785 bp
  • C to A, chromosome 16 at 16,673,693 bp
  • A to G, chromosome 17 at 26,068,706 bp
  • G to A, chromosome 17 at 30,748,568 bp
  • A to G, chromosome 18 at 37,486,470 bp
  • T to A, chromosome 19 at 18,825,872 bp
  • T to C, chromosome 19 at 22,987,869 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4565 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041790-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.