Strain Name:
C57BL/6J-MtgxR4647Btlr/Mmmh
Stock Number:
041908-MU
Citation ID:
RRID:MMRRC_041908-MU
Other Names:
R4647 (G1), C57BL/6J-MtgxR4647Btlr
Major Collection:

Gene Information

Gene Symbol: Rptor [MGI:1921620] (Mus musculus (mouse))
Name: regulatory associated protein of MTOR, complex 1
Synonyms: Rap, 4932417H02Rik, raptor
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Aatf [MGI:1929608] (Mus musculus (mouse))
Name: apoptosis antagonizing transcription factor
Synonyms: Che-1, Trb, 5830465M17Rik, 4933415H02Rik
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Ext1 [MGI:894663] (Mus musculus (mouse))
Name: exostoses (multiple) 1
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Gabra6 [MGI:95618] (Mus musculus (mouse))
Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6
Synonyms: Gabra-6, alpha6, GABA-ARalpha6
Chromosome: 11
Alteration at locus: Chemically Induced
Gene Symbol: Sema3b [MGI:107561] (Mus musculus (mouse))
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: LUCA-1, SemA, Semaa, semaV, SemA, sema5
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Arc [MGI:88067] (Mus musculus (mouse))
Name: activity regulated cytoskeletal-associated protein
Synonyms: arg 3.1, Arc3.1
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Enah [MGI:108360] (Mus musculus (mouse))
Name: ENAH actin regulator
Synonyms: Ndpp1, Mena
Chromosome: 1
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 11,162,285 bp
  • A to G, chromosome 1 at 20,955,361 bp
  • A to T, chromosome 1 at 60,503,020 bp
  • C to T, chromosome 1 at 79,614,203 bp
  • G to A, chromosome 1 at 93,168,794 bp
  • A to T, chromosome 1 at 130,577,960 bp
  • A to G, chromosome 1 at 150,675,511 bp
  • G to T, chromosome 1 at 151,457,881 bp
  • C to T, chromosome 1 at 153,907,066 bp
  • T to C, chromosome 1 at 160,146,042 bp
  • C to A, chromosome 1 at 175,659,774 bp
  • T to C, chromosome 1 at 181,923,531 bp
  • G to A, chromosome 2 at 3,220,291 bp
  • A to T, chromosome 2 at 13,582,495 bp
  • A to T, chromosome 2 at 26,407,914 bp
  • T to C, chromosome 2 at 28,545,520 bp
  • T to A, chromosome 2 at 31,210,501 bp
  • A to T, chromosome 2 at 31,399,019 bp
  • A to G, chromosome 2 at 36,890,651 bp
  • G to A, chromosome 2 at 60,308,278 bp
  • G to T, chromosome 2 at 62,334,605 bp
  • T to G, chromosome 2 at 69,285,271 bp
  • T to A, chromosome 2 at 90,889,652 bp
  • A to G, chromosome 2 at 91,162,083 bp
  • T to A, chromosome 2 at 102,837,929 bp
  • G to A, chromosome 2 at 104,258,443 bp
  • A to T, chromosome 2 at 146,387,629 bp
  • C to G, chromosome 2 at 174,337,672 bp
  • T to G, chromosome 3 at 5,399,281 bp
  • A to T, chromosome 3 at 82,864,458 bp
  • A to T, chromosome 3 at 133,488,082 bp
  • A to T, chromosome 4 at 100,953,130 bp
  • T to C, chromosome 4 at 116,992,937 bp
  • T to C, chromosome 4 at 123,473,627 bp
  • A to G, chromosome 4 at 134,545,657 bp
  • T to C, chromosome 4 at 140,944,446 bp
  • T to C, chromosome 4 at 143,132,955 bp
  • T to C, chromosome 4 at 143,699,344 bp
  • A to G, chromosome 5 at 30,871,697 bp
  • C to T, chromosome 5 at 35,706,318 bp
  • C to A, chromosome 5 at 42,214,611 bp
  • A to T, chromosome 5 at 45,733,589 bp
  • C to A, chromosome 5 at 65,656,318 bp
  • C to T, chromosome 5 at 74,610,796 bp
  • T to A, chromosome 5 at 77,351,296 bp
  • A to T, chromosome 5 at 92,017,213 bp
  • T to A, chromosome 5 at 97,108,210 bp
  • A to G, chromosome 5 at 111,420,083 bp
  • T to A, chromosome 5 at 113,614,640 bp
  • C to T, chromosome 5 at 123,148,112 bp
  • G to A, chromosome 5 at 134,216,192 bp
  • T to A, chromosome 5 at 136,101,363 bp
  • T to A, chromosome 5 at 136,974,483 bp
  • G to A, chromosome 5 at 138,979,184 bp
  • A to G, chromosome 5 at 145,218,830 bp
  • G to T, chromosome 5 at 147,527,203 bp
  • A to T, chromosome 6 at 23,969,468 bp
  • C to T, chromosome 6 at 30,745,110 bp
  • A to G, chromosome 6 at 83,088,046 bp
  • T to A, chromosome 6 at 110,914,383 bp
  • C to T, chromosome 6 at 124,320,621 bp
  • T to A, chromosome 6 at 129,899,376 bp
  • T to A, chromosome 7 at 7,384,368 bp
  • T to A, chromosome 7 at 10,378,293 bp
  • C to T, chromosome 7 at 35,638,234 bp
  • A to G, chromosome 7 at 102,724,092 bp
  • A to C, chromosome 7 at 105,565,538 bp
  • A to T, chromosome 7 at 106,944,340 bp
  • A to G, chromosome 7 at 111,080,860 bp
  • T to A, chromosome 7 at 128,659,109 bp
  • A to G, chromosome 7 at 144,411,829 bp
  • T to C, chromosome 8 at 13,588,865 bp
  • A to T, chromosome 8 at 83,722,273 bp
  • A to G, chromosome 8 at 93,166,410 bp
  • A to G, chromosome 8 at 125,942,111 bp
  • A to T, chromosome 9 at 7,274,233 bp
  • A to G, chromosome 9 at 22,199,761 bp
  • A to G, chromosome 9 at 73,102,586 bp
  • T to A, chromosome 9 at 92,605,450 bp
  • C to A, chromosome 9 at 107,599,051 bp
  • T to C, chromosome 9 at 121,141,852 bp
  • T to A, chromosome 10 at 22,148,845 bp
  • G to T, chromosome 10 at 33,195,981 bp
  • T to C, chromosome 10 at 44,439,690 bp
  • A to T, chromosome 10 at 62,747,417 bp
  • T to C, chromosome 10 at 76,354,213 bp
  • G to T, chromosome 10 at 76,605,089 bp
  • T to A, chromosome 10 at 81,348,582 bp
  • T to A, chromosome 10 at 83,574,543 bp
  • G to T, chromosome 10 at 100,397,000 bp
  • A to G, chromosome 10 at 111,301,134 bp
  • A to G, chromosome 10 at 128,239,956 bp
  • A to G, chromosome 11 at 5,522,006 bp
  • G to T, chromosome 11 at 20,239,349 bp
  • A to C, chromosome 11 at 29,546,721 bp
  • A to G, chromosome 11 at 42,307,372 bp
  • T to A, chromosome 11 at 50,237,225 bp
  • G to A, chromosome 11 at 60,801,465 bp
  • A to C, chromosome 11 at 62,783,733 bp
  • T to A, chromosome 11 at 71,097,126 bp
  • T to A, chromosome 11 at 75,445,988 bp
  • T to A, chromosome 11 at 77,817,950 bp
  • G to T, chromosome 11 at 83,276,658 bp
  • T to C, chromosome 11 at 84,471,197 bp
  • T to G, chromosome 11 at 84,894,642 bp
  • T to C, chromosome 11 at 88,718,038 bp
  • C to T, chromosome 11 at 93,924,611 bp
  • A to C, chromosome 11 at 94,309,641 bp
  • T to A, chromosome 11 at 116,082,381 bp
  • T to A, chromosome 11 at 119,891,163 bp
  • T to A, chromosome 11 at 120,719,121 bp
  • A to T, chromosome 11 at 121,055,934 bp
  • T to G, chromosome 12 at 31,540,526 bp
  • G to T, chromosome 12 at 87,118,083 bp
  • G to A, chromosome 13 at 24,137,179 bp
  • A to T, chromosome 13 at 81,528,795 bp
  • A to T, chromosome 13 at 106,947,655 bp
  • A to G, chromosome 14 at 51,418,793 bp
  • A to G, chromosome 14 at 63,493,756 bp
  • A to T, chromosome 14 at 121,586,246 bp
  • T to A, chromosome 15 at 42,676,184 bp
  • A to G, chromosome 15 at 53,089,987 bp
  • A to T, chromosome 15 at 58,899,987 bp
  • G to A, chromosome 15 at 74,671,525 bp
  • T to G, chromosome 15 at 87,720,582 bp
  • T to A, chromosome 15 at 89,370,432 bp
  • A to T, chromosome 15 at 96,420,100 bp
  • G to T, chromosome 15 at 98,437,576 bp
  • A to T, chromosome 16 at 90,973,989 bp
  • A to G, chromosome 16 at 94,123,526 bp
  • T to A, chromosome 17 at 22,007,032 bp
  • C to T, chromosome 17 at 23,610,340 bp
  • C to G, chromosome 17 at 35,885,635 bp
  • T to A, chromosome 17 at 36,547,991 bp
  • T to A, chromosome 17 at 55,610,665 bp
  • A to T, chromosome 18 at 31,600,192 bp
  • A to T, chromosome 18 at 36,969,136 bp
  • G to A, chromosome 19 at 6,051,474 bp
  • T to A, chromosome 19 at 11,914,434 bp
  • A to G, chromosome 19 at 12,954,077 bp
  • A to G, chromosome 19 at 47,143,801 bp
  • G to T, chromosome 19 at 47,620,274 bp
  • A to T, chromosome X at 38,631,472 bp
  • G to T, chromosome X at 102,860,909 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4647 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041908-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.