MMRRC:042467-MU

T to C, chromosome 1 at 119,934,204 bp
T to A, chromosome 1 at 119,984,758 bp
T to A, chromosome 1 at 133,706,780 bp
T to C, chromosome 1 at 171,384,815 bp
T to C, chromosome 2 at 22,410,700 bp
C to T, chromosome 2 at 27,244,477 bp
T to C, chromosome 2 at 33,453,932 bp
T to A, chromosome 2 at 35,402,791 bp
T to C, chromosome 2 at 65,461,032 bp
T to C, chromosome 2 at 76,731,300 bp
T to C, chromosome 2 at 152,369,611 bp
T to C, chromosome 2 at 157,318,044 bp
A to G, chromosome 3 at 32,437,163 bp
G to T, chromosome 3 at 57,847,453 bp
A to T, chromosome 3 at 98,102,419 bp
A to G, chromosome 3 at 108,676,838 bp
A to T, chromosome 3 at 129,983,970 bp
A to T, chromosome 3 at 138,148,385 bp
C to G, chromosome 3 at 146,469,774 bp
G to A, chromosome 4 at 12,089,416 bp
A to C, chromosome 4 at 84,971,229 bp
T to C, chromosome 4 at 109,068,367 bp
T to C, chromosome 4 at 115,252,881 bp
T to C, chromosome 4 at 126,262,488 bp
G to T, chromosome 4 at 143,853,303 bp
C to T, chromosome 4 at 149,982,778 bp
A to G, chromosome 5 at 73,608,567 bp
A to T, chromosome 5 at 88,488,734 bp
G to T, chromosome 5 at 92,943,086 bp
A to G, chromosome 5 at 93,392,749 bp
G to A, chromosome 5 at 136,743,058 bp
T to C, chromosome 6 at 29,447,890 bp
T to C, chromosome 6 at 83,028,353 bp
G to A, chromosome 6 at 119,278,256 bp
A to T, chromosome 6 at 128,384,988 bp
T to A, chromosome 6 at 129,493,596 bp
G to A, chromosome 6 at 140,644,073 bp
A to C, chromosome 6 at 148,873,011 bp
T to A, chromosome 7 at 3,240,442 bp
T to C, chromosome 7 at 5,454,533 bp
A to G, chromosome 7 at 16,215,442 bp
A to G, chromosome 7 at 25,246,211 bp
T to A, chromosome 7 at 106,873,970 bp
A to T, chromosome 7 at 131,561,568 bp
T to A, chromosome 8 at 3,716,419 bp
A to C, chromosome 8 at 66,481,477 bp
A to T, chromosome 8 at 75,888,600 bp
G to A, chromosome 8 at 94,351,810 bp
T to A, chromosome 8 at 94,563,964 bp
T to A, chromosome 8 at 125,670,692 bp
T to A, chromosome 9 at 16,021,330 bp
T to C, chromosome 9 at 22,026,318 bp
A to T, chromosome 9 at 38,403,468 bp
T to A, chromosome 9 at 51,280,325 bp
T to C, chromosome 9 at 57,545,307 bp
T to C, chromosome 9 at 58,157,606 bp
T to C, chromosome 9 at 108,672,502 bp
C to T, chromosome 9 at 111,390,085 bp
C to A, chromosome 9 at 119,694,309 bp
C to T, chromosome 9 at 119,694,310 bp
T to A, chromosome 10 at 18,123,625 bp
TTTGCGCATT to TTT, chromosome 10 at 27,043,643 bp
G to T, chromosome 10 at 85,148,770 bp
A to G, chromosome 11 at 32,277,008 bp
A to G, chromosome 11 at 102,025,136 bp
T to C, chromosome 11 at 115,260,790 bp
G to A, chromosome 11 at 116,069,976 bp
C to T, chromosome 12 at 65,143,311 bp
C to A, chromosome 12 at 115,193,803 bp
C to A, chromosome 12 at 115,567,154 bp
A to G, chromosome 13 at 17,719,734 bp
G to A, chromosome 13 at 18,829,255 bp
T to C, chromosome 13 at 19,185,066 bp
A to T, chromosome 13 at 27,319,000 bp
G to A, chromosome 13 at 37,931,058 bp
A to T, chromosome 13 at 49,522,787 bp
A to G, chromosome 13 at 55,638,199 bp
A to G, chromosome 14 at 30,936,701 bp
T to A, chromosome 14 at 65,815,201 bp
T to C, chromosome 14 at 110,751,883 bp
A to G, chromosome 14 at 119,035,964 bp
T to C, chromosome 15 at 5,105,141 bp
G to T, chromosome 15 at 51,968,500 bp
T to A, chromosome 15 at 75,326,652 bp
A to G, chromosome 15 at 79,728,403 bp
G to A, chromosome 16 at 84,801,602 bp
T to C, chromosome 16 at 94,390,283 bp
G to T, chromosome 17 at 23,308,034 bp
T to A, chromosome 17 at 35,015,726 bp
A to G, chromosome 17 at 35,672,723 bp
A to T, chromosome 17 at 37,952,071 bp
A to T, chromosome 18 at 21,557,972 bp
C to T, chromosome 18 at 43,766,754 bp
G to A, chromosome 18 at 60,916,651 bp
C to T, chromosome 19 at 8,829,827 bp
T to C, chromosome 19 at 13,454,521 bp
T to A, chromosome 19 at 13,838,279 bp
C to T, chromosome 19 at 42,788,957 bp
GAGCAGCAGCAGCAGCAGCAGCAGCAGC to GAGCAGCAGCAGCAGCAGCAGCAGC, chromosome 19 at 46,083,155 bp

Genotype Determination

Genotyping Protocol(s)
Center protocol and contact for technical support will be shipped with mice.

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.

Strain Development

C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.

Suggested Control Mice

Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Strain Origin

Donor

Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference

MUTAGENETIX record for R4856 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information Expand All

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color

Black

Breeding

MMRRC Breeding System

Random intra-strain mating

Breeding Scheme(s)

When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.

Overall Breeding Performance

Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size

Undetermined

Recommended wean age

3 weeks

Order Request Information Expand All

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

MMRRC Item #	Description	Distribution Fee / Unit (US $) Shipping & Handling not included	Units	Notes
042467-MU-RESUS	Litter recovered from cryo-archive	$5,475.00 / Non-Profit	Litter	Recovered litter⁴; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

¹ The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

² An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

³ An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

⁴ The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.

Strain Detail Sheet

Strain Information Expand All

Colony and Husbandry Information Expand All

Order Request Information Expand All