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Strain Name:
C57BL/6J-MtgxR5010Btlr/Mmmh
Stock Number:
042601-MU
Citation ID:
RRID:MMRRC_042601-MU
Other Names:
R5010 (G1), C57BL/6J-MtgxR5010Btlr
Major Collection:

Strain Information

Ext1
Name: exostosin glycosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14042
HGNC: HGNC:3512
Homologene: 30957
Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: MGluT1, Eaat1, B430115D02Rik, GLAST, Gmt1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20512
Homologene: 20882
Egr1
Name: early growth response 1
Synonyms: TIS8, NGFIA, Krox24, Zenk, Zif268, ETR103, Krox-24, Krox-1, Egr-1, Zfp-6, NGF1-A, A530045N19Rik, NGFI-A
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13653
HGNC: HGNC:3238
Homologene: 56394
Lrrfip2
Name: leucine rich repeat (in FLII) interacting protein 2
Synonyms: 5133400F20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71268
HGNC: HGNC:6703
Homologene: 22476
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Med13l
Name: mediator complex subunit 13-like
Synonyms: 2210413I17Rik, 6330591G05Rik, Trap240L, 9030618F05Rik, Thrap2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76199
Homologene: 25256
Rpap1
Name: RNA polymerase II associated protein 1
Synonyms: 1190005L06Rik, A730023M06Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68925
Homologene: 32269
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 15,312,962 bp
  • A to G, chromosome 1 at 28,777,862 bp
  • A to T, chromosome 1 at 75,186,060 bp
  • G to A, chromosome 1 at 86,760,321 bp
  • A to G, chromosome 1 at 107,379,649 bp
  • A to T, chromosome 1 at 172,476,213 bp
  • A to T, chromosome 2 at 26,476,114 bp
  • A to G, chromosome 2 at 32,574,428 bp
  • T to G, chromosome 2 at 76,900,511 bp
  • T to C, chromosome 2 at 104,787,831 bp
  • T to C, chromosome 2 at 119,770,041 bp
  • C to A, chromosome 2 at 128,784,000 bp
  • G to A, chromosome 2 at 152,293,936 bp
  • T to A, chromosome 2 at 158,635,363 bp
  • T to C, chromosome 3 at 35,979,017 bp
  • T to C, chromosome 3 at 54,729,223 bp
  • T to C, chromosome 3 at 59,178,958 bp
  • T to C, chromosome 3 at 96,919,849 bp
  • T to C, chromosome 3 at 116,636,427 bp
  • T to C, chromosome 4 at 43,415,926 bp
  • T to C, chromosome 4 at 45,317,702 bp
  • T to C, chromosome 4 at 108,172,131 bp
  • T to G, chromosome 4 at 112,727,672 bp
  • T to C, chromosome 4 at 113,546,537 bp
  • C to A, chromosome 5 at 30,622,805 bp
  • G to C, chromosome 5 at 76,657,834 bp
  • C to T, chromosome 5 at 76,928,678 bp
  • T to C, chromosome 5 at 88,522,505 bp
  • T to C, chromosome 5 at 118,593,550 bp
  • T to A, chromosome 5 at 150,067,360 bp
  • T to C, chromosome 6 at 125,133,763 bp
  • T to C, chromosome 6 at 125,566,257 bp
  • T to A, chromosome 6 at 132,868,475 bp
  • T to A, chromosome 6 at 149,583,618 bp
  • A to T, chromosome 7 at 18,349,354 bp
  • A to G, chromosome 7 at 18,427,891 bp
  • A to T, chromosome 7 at 28,306,859 bp
  • T to C, chromosome 7 at 86,694,585 bp
  • T to A, chromosome 7 at 89,510,214 bp
  • G to A, chromosome 8 at 15,083,310 bp
  • G to A, chromosome 8 at 25,947,960 bp
  • C to T, chromosome 8 at 48,137,414 bp
  • T to A, chromosome 9 at 40,670,648 bp
  • T to A, chromosome 9 at 63,953,900 bp
  • T to A, chromosome 9 at 67,916,379 bp
  • T to C, chromosome 9 at 109,403,424 bp
  • T to C, chromosome 9 at 111,223,972 bp
  • T to C, chromosome 9 at 122,852,345 bp
  • A to G, chromosome 10 at 93,065,118 bp
  • G to A, chromosome 11 at 3,252,249 bp
  • C to T, chromosome 11 at 58,422,804 bp
  • T to A, chromosome 11 at 88,941,349 bp
  • T to C, chromosome 11 at 105,780,092 bp
  • C to T, chromosome 12 at 64,957,227 bp
  • T to A, chromosome 13 at 33,671,444 bp
  • A to G, chromosome 13 at 95,673,743 bp
  • A to G, chromosome 14 at 43,962,845 bp
  • T to C, chromosome 15 at 8,650,846 bp
  • A to T, chromosome 15 at 36,555,324 bp
  • T to A, chromosome 15 at 53,092,412 bp
  • G to A, chromosome 17 at 66,147,722 bp
  • C to A, chromosome 17 at 87,723,413 bp
  • A to G, chromosome 18 at 14,822,172 bp
  • A to C, chromosome 18 at 34,578,685 bp
  • A to G, chromosome 18 at 34,863,658 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5010 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042601-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.