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Strain Name:
C57BL/6J-MtgxR5159Btlr/Mmmh
Stock Number:
042741-MU
Citation ID:
RRID:MMRRC_042741-MU
Other Names:
R5159 (G1), C57BL/6J-MtgxR5159Btlr
Major Collection:

Strain Information

Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Por
Name: cytochrome p450 oxidoreductase
Synonyms: NADH cytochrome P450 oxydoreductase, CYPOR, CPR, 4933424M13Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18984
HGNC: HGNC:9208
Homologene: 725
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Smc2
Name: structural maintenance of chromosomes 2
Synonyms: 5730502P04Rik, CAP-E, Fin16, Smc2l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14211
Homologene: 4705
Ireb2
Name: iron responsive element binding protein 2
Synonyms: Irp2, D9Ertd85e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 64602
VEGA: 9
HGNC: HGNC:6115
Homologene: 11280
Tmem87b
Name: transmembrane protein 87B
Synonyms: 2810431I02Rik, 2610301K12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72477
Homologene: 69513
Ube2k
Name: ubiquitin-conjugating enzyme E2K
Synonyms: D5Ertd601e, Hip2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53323
HGNC: HGNC:4914
Homologene: 3903
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,346,203 bp
  • A to G, chromosome 1 at 45,386,831 bp
  • T to C, chromosome 1 at 72,585,923 bp
  • A to T, chromosome 1 at 74,741,487 bp
  • T to C, chromosome 1 at 87,676,342 bp
  • A to C, chromosome 1 at 139,243,018 bp
  • A to G, chromosome 1 at 158,761,619 bp
  • A to G, chromosome 2 at 128,824,458 bp
  • C to T, chromosome 3 at 20,227,223 bp
  • A to G, chromosome 4 at 32,774,008 bp
  • G to A, chromosome 4 at 52,460,181 bp
  • G to A, chromosome 4 at 60,221,062 bp
  • C to T, chromosome 4 at 135,425,281 bp
  • T to C, chromosome 5 at 65,571,637 bp
  • A to T, chromosome 5 at 135,730,917 bp
  • T to A, chromosome 5 at 136,995,078 bp
  • T to G, chromosome 5 at 150,542,108 bp
  • A to G, chromosome 6 at 124,699,394 bp
  • T to C, chromosome 7 at 19,785,308 bp
  • T to A, chromosome 7 at 28,153,308 bp
  • C to T, chromosome 7 at 29,829,767 bp
  • A to T, chromosome 7 at 126,703,049 bp
  • A to T, chromosome 8 at 45,795,730 bp
  • T to C, chromosome 8 at 47,900,761 bp
  • T to C, chromosome 8 at 69,138,584 bp
  • T to C, chromosome 8 at 124,896,213 bp
  • A to G, chromosome 9 at 54,892,547 bp
  • T to A, chromosome 9 at 70,812,910 bp
  • T to A, chromosome 9 at 75,893,753 bp
  • A to T, chromosome 9 at 121,460,412 bp
  • A to G, chromosome 10 at 42,008,715 bp
  • A to G, chromosome 10 at 127,020,448 bp
  • T to C, chromosome 11 at 48,948,525 bp
  • T to C, chromosome 11 at 67,288,353 bp
  • A to G, chromosome 11 at 80,220,272 bp
  • T to C, chromosome 11 at 88,999,532 bp
  • C to T, chromosome 11 at 94,742,060 bp
  • T to C, chromosome 11 at 100,094,049 bp
  • A to T, chromosome 12 at 104,149,512 bp
  • C to A, chromosome 13 at 6,567,471 bp
  • T to A, chromosome 13 at 22,620,605 bp
  • T to A, chromosome 13 at 24,913,793 bp
  • T to A, chromosome 13 at 55,717,921 bp
  • A to G, chromosome 13 at 63,321,865 bp
  • G to A, chromosome 14 at 67,792,289 bp
  • T to C, chromosome 14 at 69,697,604 bp
  • T to C, chromosome 16 at 36,452,219 bp
  • T to C, chromosome 16 at 48,734,937 bp
  • T to A, chromosome 16 at 52,112,120 bp
  • T to C, chromosome 16 at 79,003,410 bp
  • T to A, chromosome 16 at 92,528,066 bp
  • T to G, chromosome 17 at 24,418,512 bp
  • T to C, chromosome 17 at 25,751,589 bp
  • A to G, chromosome 17 at 27,881,556 bp
  • A to C, chromosome 17 at 28,359,060 bp
  • A to C, chromosome 17 at 46,217,609 bp
  • T to C, chromosome 18 at 37,266,363 bp
  • A to G, chromosome 18 at 37,695,666 bp
  • A to G, chromosome 18 at 40,726,375 bp
  • A to G, chromosome 19 at 4,737,857 bp
  • A to G, chromosome 19 at 39,560,934 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5159 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042741-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.