Strain Name:
C57BL/6J-MtgxR5246Btlr/Mmmh
Stock Number:
042817-MU
Citation ID:
RRID:MMRRC_042817-MU
Other Names:
R5246 (G1), C57BL/6J-MtgxR5246Btlr
Major Collection:

Strain Information

Cep55
Name: centrosomal protein 55
Synonyms: 2700032M20Rik, 1200008O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74107
VEGA: 19
HGNC: HGNC:1161
Homologene: 10019
Scrib
Name: scribbled planar cell polarity
Synonyms: Scrb1, Crc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105782
Homologene: 44228
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: med, seal, motor end-plate disease, nmf2, ataxia 3, mnd2, mnd-2, C630029C19Rik, nmf58, NaCh6, Nav1.6, nmf335, NMF335, nur14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20273
Homologene: 7927
Septin7
Name: septin 7
Synonyms: Cdc10, Sept7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235072
VEGA: 9
HGNC: HGNC:1717
Homologene: 1354
Brwd1
Name: bromodomain and WD repeat domain containing 1
Synonyms: 5330419I02Rik, D530019K20Rik, G1-403-16, Wdr9, repro5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 93871
Homologene: 23130
Bltp1
Name: bridge-like lipid transfer protein family member 1
Synonyms: Tweek, FSA, 4932438A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229227
Homologene: 52105
Eif3c
Name: eukaryotic translation initiation factor 3, subunit C
Synonyms: NIPIL(A3), 110kDa, 3230401O13Rik, Eif3s8, Xsl, Xs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56347
Homologene: 2781
Zfp292
Name: zinc finger protein 292
Synonyms: Zn-16, Zn-15, Krox-10, Zfp-15, 9430062L07Rik, 5730450D02Rik, Zfp15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 30046
Homologene: 8493
Spry1
Name: sprouty RTK signaling antagonist 1
Synonyms: sprouty1, sprouty 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 24063
Homologene: 4266
Wdr25
Name: WD repeat domain 25
Synonyms: B930090D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 212198
VEGA: 12
Homologene: 56993
Clhc1
Name: clathrin heavy chain linker domain containing 1
Synonyms: 1700034F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 73324
Homologene: 17569
Epb41l4b
Name: erythrocyte membrane protein band 4.1 like 4b
Synonyms: Ehm2, D4Ertd346e, 6430543G08Rik, Lulu2, Epb4.1l4b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 54357
Homologene: 69270
Myo16
Name: myosin XVI
Synonyms: C230040D10Rik, Nyap3, BM140241
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244281
Homologene: 34710
Prss46
Name: protease, serine 46
Synonyms: 1700112C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74306
Homologene: 77706
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Cdkl4
Name: cyclin-dependent kinase-like 4
Synonyms: LOC381113
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381113
VEGA: 17
Homologene: 28725
Rint1
Name: RAD50 interactor 1
Synonyms: 2810450M21Rik, 1500019C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72772
Homologene: 11070
Rnf139
Name: ring finger protein 139
Synonyms: 4930555P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75841
VEGA: 15
Homologene: 5222
Sdk1
Name: sidekick cell adhesion molecule 1
Synonyms: 6720466O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330222
Homologene: 27395
Mup5
Name: major urinary protein 5
Synonyms: Mup V
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17844
Homologene: 74304
Adgrg6
Name: adhesion G protein-coupled receptor G6
Synonyms: LOC215798, 1190004A11Rik, DREG, Gpr126
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215798
Homologene: 10724
Efcab5
Name: EF-hand calcium binding domain 5
Synonyms: 4930563A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319634
Homologene: 63366
Gm9776
Name: predicted gene 9776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 328309
VEGA: 13
Vmn2r45
Name: vomeronasal 2, receptor 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042810
Homologene: 113703
Tmcc1
Name: transmembrane and coiled coil domains 1
Synonyms: 3632431M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330401
Homologene: 8995
Atp8b2
Name: ATPase, class I, type 8B, member 2
Synonyms: Id
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 54667
Homologene: 23226
Cybb
Name: cytochrome b-245, beta polypeptide
Synonyms: gp91phox, gp91phox, Nox2, Cgd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 13058
HGNC: HGNC:2578
Homologene: 68054
Rptn
Name: repetin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20129
Homologene: 84780
Srpk3
Name: serine/arginine-rich protein specific kinase 3
Synonyms: Mssk1, Stk23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 56504
Homologene: 69164
Gm20528
Name: predicted gene 20528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Lrcol1
Name: leucine rich colipase-like 1
Synonyms: Gm1679
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381667
Homologene: 128618
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • A to G, chromosome 2 at 41,470,940 bp
  • A to C, chromosome 2 at 42,652,951 bp
  • G to A, chromosome 3 at 37,048,050 bp
  • A to G, chromosome 3 at 37,642,767 bp
  • A to T, chromosome 3 at 89,947,966 bp
  • A to T, chromosome 3 at 93,396,833 bp
  • A to G, chromosome 3 at 93,397,729 bp
  • A to C, chromosome 4 at 34,805,842 bp
  • G to T, chromosome 4 at 57,040,989 bp
  • T to A, chromosome 4 at 61,834,637 bp
  • G to T, chromosome 4 at 143,655,557 bp
  • T to A, chromosome 5 at 23,800,811 bp
  • A to C, chromosome 5 at 110,354,511 bp
  • C to A, chromosome 5 at 142,114,562 bp
  • A to G, chromosome 6 at 116,043,420 bp
  • T to G, chromosome 7 at 8,483,252 bp
  • G to A, chromosome 7 at 126,557,238 bp
  • A to T, chromosome 8 at 10,562,212 bp
  • T to A, chromosome 9 at 25,299,536 bp
  • T to A, chromosome 9 at 110,850,034 bp
  • T to A, chromosome 10 at 14,426,765 bp
  • T to A, chromosome 11 at 29,575,434 bp
  • T to C, chromosome 11 at 77,188,845 bp
  • A to G, chromosome 12 at 109,027,456 bp
  • A to T, chromosome 13 at 94,358,557 bp
  • G to A, chromosome 15 at 58,899,703 bp
  • T to C, chromosome 15 at 76,064,797 bp
  • T to C, chromosome 15 at 101,011,057 bp
  • G to A, chromosome 16 at 96,002,557 bp
  • T to A, chromosome 17 at 80,539,484 bp
  • T to A, chromosome 19 at 38,069,671 bp
  • C to G, chromosome X at 9,450,750 bp
  • G to T, chromosome X at 73,774,948 bp
  • C to T, chromosome X at 73,774,949 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5246 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042817-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.