Strain Name:
C57BL/6J-MtgxR5274Btlr/Mmmh
Stock Number:
042837-MU
Citation ID:
RRID:MMRRC_042837-MU
Other Names:
R5274 (G1), C57BL/6J-MtgxR5274Btlr
Major Collection:

Strain Information

Slc26a5
Name: solute carrier family 26, member 5
Synonyms: prestin, Pres
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Eomes
Name: eomesodermin
Synonyms: Tbr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13813
HGNC: HGNC:3372
Homologene: 3971
Ubap2l
Name: ubiquitin-associated protein 2-like
Synonyms: NICE-4, 3110083O19Rik, A430103N23Rik, 4932431F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74383
Homologene: 136291
Sfswap
Name: splicing factor SWAP
Synonyms: 6330437E22Rik, Sfrs8, 1190005N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231769
Homologene: 134548
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12695
Homologene: 72199
Lig3
Name: ligase III, DNA, ATP-dependent
Synonyms: D11Wsu78e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16882
HGNC: HGNC:6600
Homologene: 32109
Fdft1
Name: farnesyl diphosphate farnesyl transferase 1
Synonyms: Ss, SQS, squalene synthase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14137
VEGA: 14
HGNC: HGNC:3629
Homologene: 3281
Ss18
Name: SS18, subunit of BAF chromatin remodeling complex
Synonyms: Ssxt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 268996
VEGA: 18
Homologene: 38080
Usp15
Name: ubiquitin specific peptidase 15
Synonyms: 4921514G19Rik, E430033I05Rik, Gcap18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14479
VEGA: 10
Homologene: 101542
Dip2b
Name: disco interacting protein 2 homolog B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239667
Homologene: 72227
Fbxo38
Name: F-box protein 38
Synonyms: SP329, 6030410I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 107035
VEGA: 18
Homologene: 34526
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
E2f8
Name: E2F transcription factor 8
Synonyms: 4432406C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108961
Homologene: 11654
Rnf123
Name: ring finger protein 123
Synonyms: KPC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 84585
Homologene: 11112
Snx29
Name: sorting nexin 29
Synonyms: LOC381035, Gm11170, 4933437K13Rik, Rundc2a, LOC385605
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74478
Homologene: 32706
Rad1
Name: RAD1 checkpoint DNA exonuclease
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19355
HGNC: HGNC:9806
Homologene: 37695
Ighmbp2
Name: immunoglobulin mu DNA binding protein 2
Synonyms: Smubp2, Smbp-2, RIPE3b1, p110 subunit, AEP, sma, Smbp2, Catf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 20589
HGNC: HGNC:5542
Homologene: 1642
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: 4432416O06Rik, m407Asp, D330044F14Rik, Dnchc2, DHC1b, b2b414Clo, m152Asp, D030010H02Rik, DHC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Brca2
Name: breast cancer 2, early onset
Synonyms: RAB163, Fancd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12190
HGNC: HGNC:1101
Homologene: 41
Vps51
Name: VPS51 GARP complex subunit
Synonyms: 1110014N23Rik, 3110057M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 68505
HGNC: HGNC:1172
Homologene: 34675
Cyb5r1
Name: cytochrome b5 reductase 1
Synonyms: 1500005G05Rik, B5R.1, Nqo3a2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72017
Homologene: 96059
Dync1li1
Name: dynein cytoplasmic 1 light intermediate chain 1
Synonyms: Dlic1, 1110053F02Rik, LIC-1, Dnclic1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235661
VEGA: 9
Homologene: 9403
Gm14325
Name: predicted gene 14325
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329575
Homologene: 134324
Zp2
Name: zona pellucida glycoprotein 2
Synonyms: Zp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22787
Homologene: 48194
Esyt3
Name: extended synaptotagmin-like protein 3
Synonyms: Fam62c, D9Ertd280e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 272636
Homologene: 18626
Pik3ap1
Name: phosphoinositide-3-kinase adaptor protein 1
Synonyms: BCAP, 1810044J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 83490
VEGA: 19
Homologene: 12848
Sox17
Name: SRY (sex determining region Y)-box 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20671
Homologene: 7948
Col24a1
Name: collagen, type XXIV, alpha 1
Synonyms: 5430404K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71355
Homologene: 65061
Sall3
Name: spalt like transcription factor 3
Synonyms: B130022O04Rik, Msal, Salt, Spalt, Msal-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20689
VEGA: 18
Homologene: 18142
Prkg2
Name: protein kinase, cGMP-dependent, type II
Synonyms: Prkgr2, cGK-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19092
HGNC: HGNC:9416
Homologene: 4556
Nlrp4a
Name: NLR family, pyrin domain containing 4A
Synonyms: E330028A19Rik, Nalp-eta, Nalp4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243880
Homologene: 79696
Gm5901
Name: predicted gene 5901
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100503879
Homologene: 18311
Agl
Name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Synonyms: 9430004C13Rik, 9630046L06Rik, 1110061O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77559
HGNC: HGNC:321
Homologene: 536
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Ttc21b
Name: tetratricopeptide repeat domain 21B
Synonyms: aln, Thm1, 2410066K11Rik, line 158
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 73668
Homologene: 57006
AY761185
Name: cDNA sequence AY761185
Synonyms: CRS4C-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100503970
Ceacam23
Name: CEA cell adhesion moleculen23
Synonyms: Gm5155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381852
Homologene: 115938
Cby2
Name: chibby family member 2
Synonyms: Nurit, Spert, 1700086N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67926
VEGA: 14
Homologene: 12213
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: C430014K11Rik, Mll4, Mll2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Sema5b
Name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Synonyms: SemG, Semag, SemG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 20357
Homologene: 8427
Plch2
Name: phospholipase C, eta 2
Synonyms: PLCeta2, Plcl4, A930027K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269615
Homologene: 85172
Cd96
Name: CD96 antigen
Synonyms: 1700109I12Rik, Tactile
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 84544
Homologene: 68489
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: alpha1E, Cchra1, Cav2.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Dynlt5
Name: dynein light chain Tctex-type 5
Synonyms: Tctex1d1, 1700055O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67344
Homologene: 12117
Rims3
Name: regulating synaptic membrane exocytosis 3
Synonyms: Rim3, A730060M23Rik, Nim3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242662
Homologene: 8837
Sectm1b
Name: secreted and transmembrane 1B
Synonyms: Sectm1, K12, 1810003C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 58210
Homologene: 86740
Chil5
Name: chitinase-like 5
Synonyms: Bclp2, Chi3l7, Bclp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229687
Homologene: 132668
Vmn2r22
Name: vomeronasal 2, receptor 22
Synonyms: EG546913
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 546913
Homologene: 84037
Zdhhc1
Name: zinc finger, DHHC domain containing 1
Synonyms: 4432412D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70796
Homologene: 36891
Klk6
Name: kallikrein related-peptidase 6
Synonyms: protease M, Klk29, Prss18, Prss9, Bssp, neurosin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19144
HGNC: HGNC:6367
Homologene: 68279
Ace
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Synonyms: CD143
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11421
HGNC: HGNC:2707
Homologene: 37351
4921539E11Rik
Name: RIKEN cDNA 4921539E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70941
Homologene: 52843
Gm4871
Name: predicted gene 4871
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231885
Homologene: 86127
Vmn1r34
Name: vomeronasal 1 receptor 34
Synonyms: Gm5991
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 546901
Homologene: 110800
Ifih1
Name: interferon induced with helicase C domain 1
Synonyms: Helicard, MDA5, 9130009C22Rik, MDA-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71586
Homologene: 32535
Tas2r121
Name: taste receptor, type 2, member 121
Synonyms: T2R21, mGR21, mT2r48, Tas2r21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387349
Homologene: 41542
Or5p64
Name: olfactory receptor family 5 subfamily P member 64
Synonyms: Olfr488, GA_x6K02T2PBJ9-10586187-10585243, MOR204-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258727
Homologene: 110483
Zfp758
Name: zinc finger protein 758
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224598
Homologene: 138293
Or52n20
Name: olfactory receptor family 52 subfamily N member 20
Synonyms: MOR34-4, Olfr659, GA_x6K02T2PBJ9-7298889-7299857
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259052
Homologene: 74118
Trub2
Name: TruB pseudouridine (psi) synthase family member 2
Synonyms: G430055L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227682
Homologene: 41056
Cbarp
Name: calcium channel, voltage-dependent, beta subunit associated regulatory protein
Synonyms: R29144/1, Dos
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 100503659
Homologene: 82309
Pacc1
Name: proton activated chloride channel 1
Synonyms: 2310028N02Rik, Tmem206
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66950
Homologene: 10097
Trav9n-1
Name: T cell receptor alpha variable 9N-1
Synonyms: Gm5074
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 279872
Pnma2
Name: paraneoplastic antigen MA2
Synonyms: A830049P17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239157
HGNC: HGNC:9159
Homologene: 5249
Rrm2
Name: ribonucleotide reductase M2
Synonyms: R2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20135
Homologene: 20277
Gm5069
Name: predicted pseudogene 5069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 277333
Pcdhga12
Name: protocadherin gamma subfamily A, 12
Synonyms: Pcdh13, pc2c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93724
HGNC: HGNC:8699
Homologene: 134588
Mroh6
Name: maestro heat-like repeat family member 6
Synonyms: Gm19570
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223645
Homologene: 90414
mmu-mir-7661
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 4,491,888 bp
  • T to C, chromosome 1 at 134,406,333 bp
  • T to C, chromosome 1 at 154,700,504 bp
  • T to C, chromosome 1 at 180,327,235 bp
  • T to C, chromosome 1 at 191,348,468 bp
  • C to T, chromosome 2 at 29,779,806 bp
  • T to C, chromosome 2 at 41,344,444 bp
  • T to C, chromosome 2 at 62,611,718 bp
  • T to C, chromosome 2 at 66,236,283 bp
  • G to A, chromosome 2 at 177,832,984 bp
  • A to G, chromosome 3 at 90,012,730 bp
  • A to C, chromosome 3 at 106,028,853 bp
  • A to G, chromosome 3 at 116,772,486 bp
  • A to C, chromosome 3 at 145,484,678 bp
  • G to C, chromosome 4 at 98,518,981 bp
  • A to T, chromosome 4 at 103,002,571 bp
  • C to T, chromosome 4 at 103,235,659 bp
  • A to G, chromosome 4 at 120,891,374 bp
  • A to T, chromosome 4 at 154,998,954 bp
  • T to C, chromosome 5 at 21,813,901 bp
  • T to A, chromosome 5 at 98,969,991 bp
  • T to C, chromosome 5 at 129,501,630 bp
  • C to G, chromosome 5 at 145,030,370 bp
  • T to C, chromosome 5 at 150,539,689 bp
  • T to G, chromosome 6 at 66,637,139 bp
  • T to C, chromosome 6 at 108,489,779 bp
  • A to T, chromosome 6 at 123,650,634 bp
  • A to G, chromosome 6 at 132,700,848 bp
  • T to C, chromosome 7 at 17,915,717 bp
  • C to T, chromosome 7 at 26,462,502 bp
  • C to G, chromosome 7 at 43,829,129 bp
  • C to T, chromosome 7 at 48,867,177 bp
  • T to C, chromosome 7 at 104,671,526 bp
  • C to A, chromosome 7 at 105,377,448 bp
  • A to T, chromosome 7 at 108,255,635 bp
  • T to C, chromosome 7 at 120,138,092 bp
  • T to C, chromosome 8 at 20,943,873 bp
  • T to C, chromosome 8 at 105,483,770 bp
  • T to C, chromosome 9 at 7,116,540 bp
  • T to A, chromosome 9 at 66,399,409 bp
  • T to C, chromosome 9 at 99,318,297 bp
  • AT to ATT, chromosome 9 at 108,064,003 bp
  • T to C, chromosome 9 at 114,715,205 bp
  • T to C, chromosome 9 at 118,480,529 bp
  • T to C, chromosome 10 at 80,131,815 bp
  • C to A, chromosome 10 at 123,168,351 bp
  • T to A, chromosome 11 at 82,797,292 bp
  • T to C, chromosome 11 at 105,968,037 bp
  • A to G, chromosome 11 at 121,055,900 bp
  • T to A, chromosome 12 at 24,710,407 bp
  • A to T, chromosome 14 at 53,101,835 bp
  • A to G, chromosome 14 at 63,152,343 bp
  • G to T, chromosome 14 at 66,916,760 bp
  • A to G, chromosome 14 at 75,583,226 bp
  • T to A, chromosome 15 at 10,487,973 bp
  • A to G, chromosome 15 at 75,885,000 bp
  • A to G, chromosome 15 at 98,854,230 bp
  • A to G, chromosome 15 at 100,212,104 bp
  • G to T, chromosome 16 at 11,738,404 bp
  • A to G, chromosome 16 at 35,658,221 bp
  • G to T, chromosome 16 at 46,069,703 bp
  • T to A, chromosome 17 at 22,375,855 bp
  • G to A, chromosome 18 at 14,641,049 bp
  • T to A, chromosome 18 at 37,766,422 bp
  • A to T, chromosome 18 at 62,515,069 bp
  • T to C, chromosome 18 at 80,969,837 bp
  • T to C, chromosome 19 at 3,265,518 bp
  • T to G, chromosome 19 at 6,071,033 bp
  • T to C, chromosome 19 at 41,281,952 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5274 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042837-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.