Strain Name:
C57BL/6J-MtgxR5257Btlr/Mmmh
Stock Number:
042855-MU
Citation ID:
RRID:MMRRC_042855-MU
Other Names:
R5257 (G1), C57BL/6J-MtgxR5257Btlr
Major Collection:

Gene Information

Slc6a5
Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Synonyms: Glyt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 104245
Homologene: 37901
Card11
Name: caspase recruitment domain family, member 11
Synonyms: 0610008L17Rik, CARMA1, BIMP3, 2410011D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108723
Homologene: 13024
Prdm16
Name: PR domain containing 16
Synonyms: 5730557K01Rik, Mel1, csp1, line 27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70673
Homologene: 11139
P2rx7
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7 receptor, P2X7R, P2X(7)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18439
HGNC: HGNC:8537
Homologene: 1925
Ptpn14
Name: protein tyrosine phosphatase, non-receptor type 14
Synonyms: OTTMUSG00000022087, PTP36, C130080N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19250
HGNC: HGNC:9647
Homologene: 3941
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: Hsn2, Prkwnk1, EG406236, 6430573H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232341
Homologene: 14253
Gdf3
Name: growth differentiation factor 3
Synonyms: Vgr2, Vgr-2, Gdf-3, ecat9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14562
HGNC: HGNC:4218
Homologene: 7336
Cnot8
Name: CCR4-NOT transcription complex, subunit 8
Synonyms: 1810022F04Rik, 1500015I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69125
HGNC: HGNC:9207
Homologene: 48304
Tfdp1
Name: transcription factor Dp 1
Synonyms: Dp1, Drtf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 21781
Homologene: 38276
Asf1b
Name: anti-silencing function 1B histone chaperone
Synonyms: 1700003K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66929
Homologene: 56797
Tex2
Name: testis expressed gene 2
Synonyms: 4930568E07Rik, Def-5, Taz4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21763
Homologene: 32414
Fgfr1op
Name: Fgfr1 oncogene partner
Synonyms: 4930553O10Rik, Fop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75296
Homologene: 5116
Afg3l2
Name: AFG3-like AAA ATPase 2
Synonyms: 2310036I02Rik, Emv66, par
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 69597
VEGA: 18
HGNC: HGNC:315
Homologene: 4947
Phf11d
Name: PHD finger protein 11D
Synonyms: D14Ertd668e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219132
Homologene: 87807
Ipo9
Name: importin 9
Synonyms: Imp9, 0710008K06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226432
Homologene: 5874
Foxi2
Name: forkhead box I2
Synonyms: B130055A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 270004
Homologene: 18838
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: Coh1, 1810042B05Rik, 2310042E16Rik, C330002D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Slc18a3
Name: solute carrier family 18 (vesicular monoamine), member 3
Synonyms: VAT, VAChT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 20508
Homologene: 11022
Zfp958
Name: zinc finger protein 958
Synonyms: BC003267
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 233987
Homologene: 129582
Nle1
Name: notchless homolog 1
Synonyms: Nle, l11Jus4, l11Jus1, notchless
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217011
Homologene: 5494
Abca16
Name: ATP-binding cassette, sub-family A (ABC1), member 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233810
Homologene: 132942
Mcoln1
Name: mucolipin 1
Synonyms: 2210015I05Rik, TRPML1, mucolipidin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94178
Homologene: 10744
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328783
VEGA: 17
Homologene: 18633
Psca
Name: prostate stem cell antigen
Synonyms: 2210408B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72373
HGNC: HGNC:9500
Homologene: 4150
Abca13
Name: ATP-binding cassette, sub-family A (ABC1), member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268379
Homologene: 27991
Chsy3
Name: chondroitin sulfate synthase 3
Synonyms: 4833446K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 78923
VEGA: 18
Homologene: 28624
Lyzl6
Name: lysozyme-like 6
Synonyms: 1700023H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69444
Homologene: 10709
Astn1
Name: astrotactin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11899
HGNC: HGNC:773
Homologene: 7233
Dock3
Name: dedicator of cyto-kinesis 3
Synonyms: Moca, PBP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208869
HGNC: HGNC:2989
Homologene: 21030
Slc44a5
Name: solute carrier family 44, member 5
Synonyms: LOC242259
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242259
Homologene: 72094
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242627
Homologene: 135888
Stard9
Name: START domain containing 9
Synonyms: 4831403C07Rik, N-3 kinesin, E230025N21Rik, Kif16a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668880
Homologene: 130712
Nckap5
Name: NCK-associated protein 5
Synonyms: LOC380609, D130011D22Rik, E030049G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 210356
Homologene: 35542
Pla2g2c
Name: phospholipase A2, group IIC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18781
HGNC: HGNC:9032
Homologene: 7477
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 214321
Homologene: 86950
Acpp
Name: acid phosphatase, prostate
Synonyms: PAP, A030005E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56318
HGNC: HGNC:125
Homologene: 55552
Olfr221
Name: olfactory receptor 221
Synonyms: GA_x6K02SYYB8M-929-258, Olfr1514, MOR205-2, MOR205-1, GA_x6K02T2RJGY-959918-960853
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258420
Homologene: 45823
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243369
Homologene: 45453
Zfp521
Name: zinc finger protein 521
Synonyms: B930086A16Rik, Evi3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225207
VEGA: 18
Homologene: 9151
Dsg1a
Name: desmoglein 1 alpha
Synonyms: Dsg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13510
HGNC: HGNC:3048
Homologene: 1463
Sardh
Name: sarcosine dehydrogenase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 192166
Homologene: 5149
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Klk14
Name: kallikrein related-peptidase 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 317653
HGNC: HGNC:6362
Homologene: 69348
Ptrhd1
Name: peptidyl-tRNA hydrolase domain containing 1
Synonyms: 2410017P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 69709
VEGA: 12
Homologene: 13963
Setd4
Name: SET domain containing 4
Synonyms: ORF21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224440
HGNC: HGNC:1258
Homologene: 41173
Padi4
Name: peptidyl arginine deiminase, type IV
Synonyms: PAD type IV, Pdi4, Pad4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18602
Homologene: 7883
Sirpb1b
Name: signal-regulatory protein beta 1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 668101
Homologene: 82993
Dkk4
Name: dickkopf WNT signaling pathway inhibitor 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234130
HGNC: HGNC:2894
Homologene: 8680
Sorbs3
Name: sorbin and SH3 domain containing 3
Synonyms: SH3P3, vinexin alpha, vinexin beta, Sh3d4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 20410
VEGA: 14
Homologene: 4218
4921506M07Rik
Name: RIKEN cDNA 4921506M07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Kyat3
Name: kynurenine aminotransferase 3
Synonyms: Ccbl2, KATIII, Kat3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229905
Homologene: 2994
Sesn1
Name: sestrin 1
Synonyms: 1110002G11Rik, PA26, SEST1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 140742
Homologene: 8697
Dcaf5
Name: DDB1 and CUL4 associated factor 5
Synonyms: BCRP2, Wdr22, BCRG2, 9430020B07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320808
VEGA: 12
Homologene: 18564
Mfsd4b2
Name: major facilitator superfamily domain containing 4B2
Synonyms: Mfsd4b2, Mfsd4b2-ps, 2010001E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 72045
Homologene: 129931
Gm21738
Name: predicted gene, 21738
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Igkv4-80
Name: immunoglobulin kappa variable 4-80
Synonyms: Gm16729
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 545848
Lbhd1
Name: LBH domain containing 1
Synonyms: Gm21743
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 102308570
Homologene: 75203
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 126,024,508 bp
  • A to T, chromosome 1 at 135,385,435 bp
  • A to G, chromosome 1 at 158,612,532 bp
  • A to G, chromosome 1 at 189,851,402 bp
  • T to C, chromosome 2 at 27,244,259 bp
  • T to A, chromosome 2 at 120,699,343 bp
  • T to C, chromosome 3 at 15,548,757 bp
  • A to G, chromosome 3 at 142,734,576 bp
  • G to A, chromosome 3 at 154,243,123 bp
  • T to C, chromosome 4 at 113,577,662 bp
  • G to A, chromosome 4 at 138,731,545 bp
  • C to T, chromosome 4 at 140,746,204 bp
  • A to T, chromosome 4 at 154,367,214 bp
  • A to G, chromosome 5 at 122,681,003 bp
  • G to A, chromosome 5 at 140,876,425 bp
  • T to C, chromosome 6 at 48,476,494 bp
  • T to A, chromosome 6 at 69,016,827 bp
  • A to G, chromosome 6 at 120,037,188 bp
  • T to C, chromosome 6 at 122,606,386 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • G to A, chromosome 7 at 49,929,992 bp
  • C to T, chromosome 7 at 105,674,037 bp
  • T to G, chromosome 7 at 120,436,769 bp
  • C to T, chromosome 7 at 135,410,527 bp
  • G to A, chromosome 8 at 3,512,718 bp
  • A to T, chromosome 8 at 4,628,456 bp
  • C to T, chromosome 8 at 13,369,529 bp
  • C to A, chromosome 8 at 22,627,015 bp
  • C to T, chromosome 8 at 83,969,267 bp
  • T to C, chromosome 9 at 104,309,475 bp
  • T to C, chromosome 9 at 106,996,925 bp
  • T to A, chromosome 10 at 39,922,021 bp
  • C to T, chromosome 10 at 41,894,988 bp
  • A to T, chromosome 11 at 9,249,684 bp
  • T to A, chromosome 11 at 58,117,522 bp
  • G to C, chromosome 11 at 60,711,563 bp
  • T to C, chromosome 11 at 82,904,946 bp
  • T to A, chromosome 11 at 103,635,073 bp
  • T to A, chromosome 11 at 106,567,759 bp
  • A to G, chromosome 12 at 4,236,481 bp
  • A to T, chromosome 12 at 57,702,275 bp
  • G to T, chromosome 12 at 80,397,719 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • A to G, chromosome 14 at 19,415,942 bp
  • T to C, chromosome 14 at 32,463,820 bp
  • T to A, chromosome 14 at 47,667,363 bp
  • T to C, chromosome 14 at 52,035,884 bp
  • T to C, chromosome 14 at 59,352,711 bp
  • T to C, chromosome 14 at 70,185,034 bp
  • A to G, chromosome 15 at 35,794,421 bp
  • A to T, chromosome 15 at 74,716,391 bp
  • T to C, chromosome 16 at 93,596,333 bp
  • T to C, chromosome 17 at 8,172,943 bp
  • T to C, chromosome 17 at 25,746,165 bp
  • G to A, chromosome 18 at 13,846,978 bp
  • A to G, chromosome 18 at 20,320,931 bp
  • A to G, chromosome 18 at 59,409,794 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • A to G, chromosome 19 at 8,884,089 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5257 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042855-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.