Strain Name:
C57BL/6J-MtgxR5295Btlr/Mmmh
Stock Number:
042878-MU
Citation ID:
RRID:MMRRC_042878-MU
Other Names:
R5295 (G1), C57BL/6J-MtgxR5295Btlr
Major Collection:

Gene Information

Ank2
Name: ankyrin 2, brain
Synonyms: Ankyrin-2, Gm4392, Ank-2, ankyrin B, Ankyrin-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 109676
HGNC: HGNC:493
Fryl
Name: FRY like transcription coactivator
Synonyms: 2510002A14Rik, 2310004H21Rik, 9030227G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 72313
Homologene: 103956
Tdh
Name: L-threonine dehydrogenase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 58865
Homologene: 10987
Atp5c1
Name: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
Synonyms: F1 gamma, 1700094F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11949
HGNC: HGNC:833
Homologene: 3792
Tlk1
Name: tousled-like kinase 1
Synonyms: 4930545J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228012
Homologene: 130657
Rnf123
Name: ring finger protein 123
Synonyms: KPC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 84585
Homologene: 11112
St7l
Name: suppression of tumorigenicity 7-like
Synonyms: St7r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229681
Homologene: 14910
Cep135
Name: centrosomal protein 135
Synonyms: Cep4, LOC381644
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 381644
Homologene: 45709
Arhgef1
Name: Rho guanine nucleotide exchange factor (GEF) 1
Synonyms: Lsc, Lbcl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16801
HGNC: HGNC:681
Homologene: 3454
Zbtb2
Name: zinc finger and BTB domain containing 2
Synonyms: LOC381990
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 381990
VEGA: 10
Homologene: 10837
Serpina3c
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3C
Synonyms: Klkbp, 1A1, Kalbp, alpha-1 antiproteinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 16625
HGNC: HGNC:16
Homologene: 111129
Psrc1
Name: proline/serine-rich coiled-coil 1
Synonyms: 5430413I02Rik, DDA3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 56742
Homologene: 49603
Vps51
Name: VPS51 GARP complex subunit
Synonyms: 1110014N23Rik, 3110057M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 68505
HGNC: HGNC:1172
Homologene: 34675
Znhit6
Name: zinc finger, HIT type 6
Synonyms: 2410019A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229937
Homologene: 32378
Dnajc16
Name: DnaJ heat shock protein family (Hsp40) member C16
Synonyms: 2900037O03Rik, 4732437J24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 214063
Homologene: 45414
Gm14325
Name: predicted gene 14325
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 329575
Homologene: 134324
Pcdh12
Name: protocadherin 12
Synonyms: vascular endothelial cadherin-2, VE-cadherin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 53601
HGNC: HGNC:8657
Homologene: 9574
Plvap
Name: plasmalemma vesicle associated protein
Synonyms: MECA32, PV-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 84094
Homologene: 10578
Zmiz1
Name: zinc finger, MIZ-type containing 1
Synonyms: Rai17, Zimp10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 328365
Homologene: 10667
Kcnv1
Name: potassium channel, subfamily V, member 1
Synonyms: 2700023A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 67498
VEGA: 15
Homologene: 22811
2410017I17Rik
Name: RIKEN cDNA 2410017I17 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 675325
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 672511
Homologene: 45439
Lrrc7
Name: leucine rich repeat containing 7
Synonyms: densin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242274
Homologene: 10817
Sfmbt1
Name: Scm-like with four mbt domains 1
Synonyms: 9330180L21Rik, 4930442N21Rik, Smr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 54650
Homologene: 9472
Perm1
Name: PPARGC1 and ESRR induced regulator, muscle 1
Synonyms: 2310042D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 74183
Homologene: 135954
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 225638
Homologene: 50475
Serpinb6c
Name: serine (or cysteine) peptidase inhibitor, clade B, member 6c
Synonyms: Spi3C, ovalbumin, SPIC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 97848
HGNC: HGNC:8950
Elovl4
Name: elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 83603
Homologene: 41488
Gad1-ps
Name: glutamate decarboxylase 1, pseudogene
Synonyms: Gad-1ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 382407
VEGA: 10
Khnyn
Name: KH and NYN domain containing
Synonyms: 9130227C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 219094
VEGA: 14
Homologene: 23611
Trim41
Name: tripartite motif-containing 41
Synonyms: RINCK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 211007
Homologene: 14140
Gm8882
Name: predicted gene 8882
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 667929
Tdrd9
Name: tudor domain containing 9
Synonyms: 4930441E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 74691
Homologene: 14311
Fam186b
Name: family with sequence similarity 186, member B
Synonyms: EG545136
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 545136
VEGA: 15
Homologene: 69502
Lmtk3
Name: lemur tyrosine kinase 3
Synonyms: Aatyk3, AATYK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 381983
Homologene: 79449
Ly6f
Name: lymphocyte antigen 6 complex, locus F
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 17071
Homologene: 113718
Alpl
Name: alkaline phosphatase, liver/bone/kidney
Synonyms: TNSALP, TNAP, Akp-2, Akp2, ALP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 11647
HGNC: HGNC:438
Homologene: 37314
Ears2
Name: glutamyl-tRNA synthetase 2, mitochondrial
Synonyms: 3230401I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 67417
Homologene: 6907
Prl7d1
Name: prolactin family 7, subfamily d, member 1
Synonyms: Plfr, PRP, PLF-RP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 18814
Homologene: 7894
Commd5
Name: COMM domain containing 5
Synonyms: D15Ertd81e, 2310065H03Rik, Hcarg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 66398
Homologene: 8548
Ccr10
Name: chemokine (C-C motif) receptor 10
Synonyms: CCR10, Cmkbr9, GPR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12777
HGNC: HGNC:4474
Homologene: 7271
Gm5117
Name: predicted gene 5117
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 330731
Ighv1-77
Name: immunoglobulin heavy variable 1-77
Synonyms: Gm16746
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 619994
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 10,068,733 bp
  • C to T, chromosome 2 at 70,752,205 bp
  • G to A, chromosome 2 at 177,832,984 bp
  • A to T, chromosome 3 at 104,891,552 bp
  • A to G, chromosome 3 at 108,386,359 bp
  • T to C, chromosome 3 at 127,032,183 bp
  • A to G, chromosome 3 at 145,600,493 bp
  • T to A, chromosome 3 at 158,170,739 bp
  • T to C, chromosome 4 at 137,749,608 bp
  • T to C, chromosome 4 at 141,767,928 bp
  • T to C, chromosome 4 at 156,217,518 bp
  • T to C, chromosome 5 at 73,112,791 bp
  • T to A, chromosome 5 at 76,593,204 bp
  • T to G, chromosome 6 at 132,361,877 bp
  • G to A, chromosome 7 at 24,919,352 bp
  • G to A, chromosome 7 at 45,791,298 bp
  • T to C, chromosome 7 at 122,048,198 bp
  • T to A, chromosome 8 at 31,739,567 bp
  • T to C, chromosome 8 at 71,511,670 bp
  • G to A, chromosome 9 at 83,780,661 bp
  • AT to ATT, chromosome 9 at 108,064,003 bp
  • T to C, chromosome 10 at 4,368,508 bp
  • A to G, chromosome 10 at 99,444,889 bp
  • TTCCTCCTCCTCCTCCTCCTCCTCCTCC to TTCCTCCTCCTCCTCCTCCTCCTCC, chromosome 11 at 48,816,257 bp
  • C to T, chromosome 11 at 101,174,285 bp
  • A to T, chromosome 11 at 119,440,816 bp
  • T to C, chromosome 12 at 104,148,378 bp
  • T to A, chromosome 12 at 112,051,912 bp
  • T to A, chromosome 12 at 115,861,908 bp
  • A to T, chromosome 13 at 27,709,247 bp
  • A to G, chromosome 13 at 33,893,817 bp
  • T to C, chromosome 14 at 25,656,347 bp
  • G to A, chromosome 14 at 30,774,029 bp
  • T to C, chromosome 14 at 55,883,463 bp
  • T to C, chromosome 14 at 63,496,109 bp
  • T to C, chromosome 15 at 45,114,591 bp
  • A to G, chromosome 15 at 75,271,639 bp
  • C to A, chromosome 15 at 76,900,952 bp
  • T to A, chromosome 15 at 99,283,874 bp
  • T to C, chromosome 17 at 36,161,675 bp
  • T to A, chromosome 18 at 38,284,101 bp
  • C to T, chromosome 18 at 65,305,038 bp
  • T to G, chromosome 19 at 6,071,033 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5295 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042878-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.