Strain Name:
C57BL/6J-MtgxR5456Btlr/Mmmh
Stock Number:
043019-MU
Citation ID:
RRID:MMRRC_043019-MU
Other Names:
R5456 (G1), C57BL/6J-MtgxR5456Btlr
Major Collection:

Gene Information

Trim13
Name: tripartite motif-containing 13
Synonyms: LEU5, RNF77, Rfp2, 3110001L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 66597
VEGA: 14
HGNC: HGNC:9976
Homologene: 4234
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 75415
Homologene: 23089
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 320528
VEGA: 9
Homologene: 41188
Bend3
Name: BEN domain containing 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 331623
VEGA: 10
Homologene: 19511
Sh3glb1
Name: SH3-domain GRB2-like B1 (endophilin)
Synonyms: Endophilin B1, Bif-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 54673
Homologene: 9337
Rarb
Name: retinoic acid receptor, beta
Synonyms: RARbeta2, Hap, RAR beta 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 218772
VEGA: 14
HGNC: HGNC:9865
Homologene: 68100
Robo2
Name: roundabout guidance receptor 2
Synonyms: 2600013A04Rik, 9430089E08Rik, D230004I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 268902
Homologene: 43188
Vegfa
Name: vascular endothelial growth factor A
Synonyms: VEGF-A, VEGF188, VEGF120, VPF, VEGF164, Vegf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 22339
Homologene: 2534
Dcbld1
Name: discoidin, CUB and LCCL domain containing 1
Synonyms: 4631413K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 66686
Homologene: 12010
Tmco3
Name: transmembrane and coiled-coil domains 3
Synonyms: B230339H12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234076
Homologene: 32376
Elfn1
Name: leucine rich repeat and fibronectin type III, extracellular 1
Synonyms: A930017N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 243312
Homologene: 18465
Cd22
Name: CD22 antigen
Synonyms: Lyb-8, Lyb8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 12483
HGNC: HGNC:1643
Homologene: 31052
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328783
VEGA: 17
HGNC: null
Homologene: 18633
Rapgef5
Name: Rap guanine nucleotide exchange factor (GEF) 5
Synonyms: mr-gef, D030051B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217944
VEGA: 12
Homologene: 56563
Poln
Name: DNA polymerase N
Synonyms: POL4P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 272158
Homologene: 131253
Umodl1
Name: uromodulin-like 1
Synonyms: D17Ertd488e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 52020
VEGA: 17
Homologene: 45466
Adcy5
Name: adenylate cyclase 5
Synonyms: AC5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 224129
VEGA: 16
HGNC: HGNC:236
Homologene: 11213
Srgap1
Name: SLIT-ROBO Rho GTPase activating protein 1
Synonyms: 4930572H05Rik, Arhgap13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 117600
Homologene: 56898
Fshr
Name: follicle stimulating hormone receptor
Synonyms: FSH-R, Follitropin receptor, follicle-stimulating hormone receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 14309
VEGA: 17
HGNC: HGNC:3969
Homologene: 117
Commd3
Name: COMM domain containing 3
Synonyms: D2Ertd542e, Bup
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12238
Homologene: 15693
Wdr83
Name: WD repeat domain containing 83
Synonyms: Morg1, 1500041N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67836
Homologene: 12194
Sel1l3
Name: sel-1 suppressor of lin-12-like 3 (C. elegans)
Synonyms: 2310045A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231238
Homologene: 9054
Baat
Name: bile acid-Coenzyme A: amino acid N-acyltransferase
Synonyms: BAT, taurine N-acyltransferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12012
HGNC: HGNC:932
Homologene: 1286
Igsf3
Name: immunoglobulin superfamily, member 3
Synonyms: 2810035F16Rik, 4833439O17Rik, 1700016K10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 78908
HGNC: HGNC:5950
Homologene: 1182
Fam83b
Name: family with sequence similarity 83, member B
Synonyms: C530008M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208994
Homologene: 19478
Nat2
Name: N-acetyltransferase 2 (arylamine N-acetyltransferase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 17961
Homologene: 37329
Usp31
Name: ubiquitin specific peptidase 31
Synonyms: 6330567E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 76179
Homologene: 44504
Ppm1f
Name: protein phosphatase 1F (PP2C domain containing)
Synonyms: 1110021B16Rik, 4933427B07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 68606
Homologene: 22828
Pabpc1l
Name: poly(A) binding protein, cytoplasmic 1-like
Synonyms: ePAB, 1810053B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 381404
Homologene: 77989
Tst
Name: thiosulfate sulfurtransferase, mitochondrial
Synonyms: Rhodanese
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 22117
VEGA: 15
Homologene: 37759
Olfr284
Name: olfactory receptor 284
Synonyms: MOR160-4, GA_x6K02T2NBG7-5395976-5396893
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 258278
HGNC: null
Homologene: 74250
Btnl2
Name: butyrophilin-like 2
Synonyms: butyrophylin-like MHC class II associated, BTL-II, BTLN2, NG9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 547431
HGNC: HGNC:1142
Homologene: 10482
Bco2
Name: beta-carotene oxygenase 2
Synonyms: beta-diox-II, Bcdo2, B-diox-II, CMO2, Bcmo2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 170752
Homologene: 12912
Olfr1257
Name: olfactory receptor 1257
Synonyms: GA_x6K02T2Q125-51319458-51320387, MOR232-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258984
Homologene: 115501
Apmap
Name: adipocyte plasma membrane associated protein
Synonyms: 2310001A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 71881
Homologene: 41380
Hemgn
Name: hemogen
Synonyms: 4921524M03Rik, EDAG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 93966
Homologene: 14223
Ear-ps2
Name: eosinophil-associated, ribonuclease A family, pseudogene 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 93743
HGNC: null
Homologene: null
4833422C13Rik
Name: RIKEN cDNA 4833422C13 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 373852
HGNC: null
Homologene: null
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 18,674,157 bp
  • G to A, chromosome 2 at 89,881,258 bp
  • T to A, chromosome 2 at 150,590,069 bp
  • T to C, chromosome 2 at 164,027,660 bp
  • C to T, chromosome 3 at 101,427,221 bp
  • T to A, chromosome 3 at 144,709,353 bp
  • C to T, chromosome 4 at 46,396,571 bp
  • C to T, chromosome 4 at 49,502,949 bp
  • A to T, chromosome 5 at 34,007,442 bp
  • T to C, chromosome 5 at 53,200,036 bp
  • A to G, chromosome 5 at 139,972,816 bp
  • T to C, chromosome 7 at 30,876,039 bp
  • T to C, chromosome 7 at 121,670,277 bp
  • A to G, chromosome 8 at 13,319,815 bp
  • G to A, chromosome 8 at 67,501,573 bp
  • T to C, chromosome 8 at 85,080,208 bp
  • T to C, chromosome 9 at 50,545,344 bp
  • T to A, chromosome 9 at 67,927,447 bp
  • T to C, chromosome 9 at 76,492,595 bp
  • T to C, chromosome 10 at 43,510,546 bp
  • A to G, chromosome 10 at 52,314,390 bp
  • G to A, chromosome 10 at 121,869,811 bp
  • T to A, chromosome 12 at 117,728,646 bp
  • A to G, chromosome 13 at 91,741,640 bp
  • G to A, chromosome 14 at 16,436,843 bp
  • C to T, chromosome 14 at 44,047,075 bp
  • A to G, chromosome 14 at 61,605,074 bp
  • T to C, chromosome 15 at 78,399,958 bp
  • G to A, chromosome 15 at 98,340,365 bp
  • T to A, chromosome 16 at 16,923,746 bp
  • T to C, chromosome 16 at 35,298,522 bp
  • T to C, chromosome 16 at 73,904,411 bp
  • A to G, chromosome 17 at 25,743,159 bp
  • A to G, chromosome 17 at 30,982,289 bp
  • A to T, chromosome 17 at 34,363,321 bp
  • C to A, chromosome 17 at 46,025,336 bp
  • T to A, chromosome 17 at 88,986,348 bp
  • G to A, chromosome 18 at 6,112,170 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5456 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043019-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.