Strain Name:
C57BL/6J-MtgxR5537Btlr/Mmmh
Stock Number:
043095-MU
Citation ID:
RRID:MMRRC_043095-MU
Other Names:
R5537 (G1), C57BL/6J-MtgxR5537Btlr
Major Collection:

Gene Information

Slc6a5
Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Synonyms: Glyt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 104245
Homologene: 37901
Crhr1
Name: corticotropin releasing hormone receptor 1
Synonyms: CRFR1, CRF-R1alpha, CRF 1 receptor, CRF1R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12921
HGNC: HGNC:2357
Homologene: 20920
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Arfgef2
Name: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99371
Homologene: 111880
Rnf40
Name: ring finger protein 40
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233900
Homologene: 8856
Ankle2
Name: ankyrin repeat and LEM domain containing 2
Synonyms: 1110001J12Rik, D5Ertd585e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71782
Homologene: 35424
Cdc37l1
Name: cell division cycle 37-like 1
Synonyms: 2700033A15Rik, Hsp90-associating relative of Cdc37, Harc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 67072
VEGA: 19
Homologene: 9912
Prom1
Name: prominin 1
Synonyms: AC133, CD133, Prom, 4932416E19Rik, Prom-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19126
HGNC: HGNC:9454
Homologene: 4390
Tox
Name: thymocyte selection-associated high mobility group box
Synonyms: 1700007F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 252838
Homologene: 8822
Iqsec3
Name: IQ motif and Sec7 domain 3
Synonyms: BRAG3, synarfGEF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243621
Homologene: 46091
Tsg101
Name: tumor susceptibility gene 101
Synonyms: CC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22088
Homologene: 4584
Adss
Name: adenylosuccinate synthetase, non muscle
Synonyms: AS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11566
HGNC: HGNC:292
Homologene: 875
Ccdc187
Name: coiled-coil domain containing 187
Synonyms: 4932418E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329366
Homologene: 77630
Pfkp
Name: phosphofructokinase, platelet
Synonyms: PFK-C, 1200015H23Rik, 9330125N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 56421
HGNC: HGNC:8878
Homologene: 20579
Eapp
Name: E2F-associated phosphoprotein
Synonyms: EAPP, 1810011O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66266
Homologene: 10206
Acsl3
Name: acyl-CoA synthetase long-chain family member 3
Synonyms: 2610510B12Rik, C85929, Facl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74205
HGNC: HGNC:3570
Homologene: 3278
Kctd12
Name: potassium channel tetramerisation domain containing 12
Synonyms: Pfet1, Pfetin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239217
Homologene: 16316
Ank1
Name: ankyrin 1, erythroid
Synonyms: Ank-1, pale
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11733
HGNC: HGNC:492
Homologene: 55427
Vps37d
Name: vacuolar protein sorting 37D
Synonyms: Wbscr24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 194309
Homologene: 45580
Slc7a2
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 2
Synonyms: Tea, Atrc2, Cat2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11988
Homologene: 20659
Tex15
Name: testis expressed gene 15
Synonyms: 2210014E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104271
Homologene: 12837
Abcb4
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 4
Synonyms: mdr-2, Mdr2, Pgy-2, Pgy2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18670
HGNC: HGNC:45
Homologene: 136368
Lrp1
Name: low density lipoprotein receptor-related protein 1
Synonyms: CD91, A2mr, b2b1554Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16971
HGNC: HGNC:6692
Homologene: 1744
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Uckl1
Name: uridine-cytidine kinase 1-like 1
Synonyms: 1110007H10Rik, Urkl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68556
Homologene: 101414
Chtf18
Name: CTF18, chromosome transmission fidelity factor 18
Synonyms: 6030457M03Rik, CTF18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 214901
Homologene: 32532
S100pbp
Name: S100P binding protein
Synonyms: 4930429A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74648
Homologene: 81892
Scarf1
Name: scavenger receptor class F, member 1
Synonyms: SREC, SREC-I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380713
Homologene: 2741
Olfr394
Name: olfactory receptor 394
Synonyms: GA_x6K02T2P1NL-4043306-4042374, MOR135-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259009
Homologene: 133669
Olfr1030
Name: olfactory receptor 1030
Synonyms: GA_x6K02T2Q125-47462755-47463693, MOR196-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258581
Homologene: 64892
Spag16
Name: sperm associated antigen 16
Synonyms: Pf20, 4930524F24Rik, 4930585K05Rik, Wdr29, 4921511D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66722
Homologene: 11572
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: C430014K11Rik, Mll4, Mll2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Mrgprx1
Name: MAS-related GPR, member X1
Synonyms: MrgC11, Mrgprc11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404242
Homologene: 86184
Cdk5r1
Name: cyclin-dependent kinase 5, regulatory subunit 1 (p35)
Synonyms: p35, D11Bwg0379e, p25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12569
HGNC: HGNC:1775
Homologene: 31200
Tubb2a
Name: tubulin, beta 2A class IIA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 22151
VEGA: 13
Homologene: 134314
Ebf4
Name: early B cell factor 4
Synonyms: Olf-1/EBF-like 4, O/E-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228598
Homologene: 65064
Zpld1
Name: zona pellucida like domain containing 1
Synonyms: 9430016A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239852
Homologene: 18401
Brinp2
Name: bone morphogenic protein/retinoic acid inducible neural-specific 2
Synonyms: 6430517E21Rik, Fam5b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240843
Homologene: 10905
Lgr5
Name: leucine rich repeat containing G protein coupled receptor 5
Synonyms: Gpr49
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14160
HGNC: HGNC:4504
Homologene: 20807
Nlrp4e
Name: NLR family, pyrin domain containing 4E
Synonyms: Nalp-epsilon, Nalp4e, 4930406H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 446099
Homologene: 75315
Clrn3
Name: clarin 3
Synonyms: Tmem12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 212070
Homologene: 17544
Tprn
Name: taperin
Synonyms: C430004E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 97031
Homologene: 52156
Olfr1307
Name: olfactory receptor 1307
Synonyms: GA_x6K02T2Q125-72988111-72987173, MOR245-19P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 257956
Homologene: 128383
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228536
Homologene: 8976
Rab14
Name: RAB14, member RAS oncogene family
Synonyms: 2810475J17Rik, 0610030G24Rik, A830021G03Rik, D030017L14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68365
Homologene: 48679
Psma5-ps
Name: proteasome subunit alpha 5, pseudogene
Synonyms: Gm8394
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 666974
VEGA: 10
Olfr902
Name: olfactory receptor 902
Synonyms: GA_x6K02T2PVTD-32141623-32142552, MOR169-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258798
VEGA: 9
Tmem182
Name: transmembrane protein 182
Synonyms: 2310079P10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381339
Homologene: 44899
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 34,189,878 bp
  • A to T, chromosome 1 at 40,854,909 bp
  • T to C, chromosome 1 at 69,827,016 bp
  • C to T, chromosome 1 at 78,706,356 bp
  • C to T, chromosome 1 at 158,255,013 bp
  • C to A, chromosome 1 at 177,796,323 bp
  • G to A, chromosome 2 at 25,263,357 bp
  • C to A, chromosome 2 at 26,276,225 bp
  • A to T, chromosome 2 at 35,192,637 bp
  • T to A, chromosome 2 at 85,984,226 bp
  • A to T, chromosome 2 at 111,945,048 bp
  • G to T, chromosome 2 at 118,915,980 bp
  • G to T, chromosome 2 at 130,362,001 bp
  • G to T, chromosome 2 at 166,856,593 bp
  • A to G, chromosome 2 at 181,570,178 bp
  • A to G, chromosome 4 at 6,697,510 bp
  • G to A, chromosome 4 at 129,182,188 bp
  • A to T, chromosome 5 at 8,955,485 bp
  • C to T, chromosome 5 at 44,000,776 bp
  • T to C, chromosome 5 at 110,249,495 bp
  • C to T, chromosome 5 at 135,074,402 bp
  • T to C, chromosome 6 at 121,412,644 bp
  • T to C, chromosome 7 at 23,320,489 bp
  • T to C, chromosome 7 at 46,891,128 bp
  • T to A, chromosome 7 at 48,021,150 bp
  • T to C, chromosome 7 at 49,959,311 bp
  • T to A, chromosome 7 at 127,596,089 bp
  • A to T, chromosome 7 at 135,514,062 bp
  • A to G, chromosome 8 at 23,114,876 bp
  • A to G, chromosome 8 at 33,571,613 bp
  • A to T, chromosome 8 at 40,913,986 bp
  • T to A, chromosome 9 at 38,449,242 bp
  • G to A, chromosome 9 at 79,699,590 bp
  • A to G, chromosome 10 at 85,314,049 bp
  • T to C, chromosome 10 at 115,456,689 bp
  • T to A, chromosome 10 at 127,605,488 bp
  • T to C, chromosome 11 at 73,887,697 bp
  • G to A, chromosome 11 at 75,525,531 bp
  • A to G, chromosome 11 at 80,477,999 bp
  • T to A, chromosome 11 at 104,163,856 bp
  • G to A, chromosome 12 at 54,692,059 bp
  • C to A, chromosome 13 at 6,619,242 bp
  • C to A, chromosome 13 at 34,075,451 bp
  • A to G, chromosome 14 at 102,982,277 bp
  • G to A, chromosome 15 at 98,852,109 bp
  • A to G, chromosome 16 at 55,233,640 bp
  • G to A, chromosome 17 at 25,719,202 bp
  • T to A, chromosome 19 at 28,995,118 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5537 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043095-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.