Strain Name:
C57BL/6J-MtgxR5624Btlr/Mmmh
Stock Number:
043163-MU
Citation ID:
RRID:MMRRC_043163-MU
Other Names:
R5624 (G1), C57BL/6J-MtgxR5624Btlr
Major Collection:

Gene Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Xrcc1
Name: X-ray repair complementing defective repair in Chinese hamster cells 1
Synonyms: Xrcc-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22594
Homologene: 31368
Ccnd1
Name: cyclin D1
Synonyms: Cyl-1, bcl-1, PRAD1, cD1, CycD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12443
HGNC: HGNC:1582
Homologene: 1334
Epb41l1
Name: erythrocyte membrane protein band 4.1 like 1
Synonyms: 4.1N, Epb4.1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13821
HGNC: HGNC:3378
Homologene: 8126
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: D630035I23Rik, TRIP8, 5430433L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 108829
Homologene: 3129
Dnajc11
Name: DnaJ heat shock protein family (Hsp40) member C11
Synonyms: E030019A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230935
Homologene: 14558
Mras
Name: muscle and microspikes RAS
Synonyms: 2900078C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17532
HGNC: HGNC:7227
Homologene: 7424
Fzd8
Name: frizzled class receptor 8
Synonyms: Fz8, mFZ8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14370
VEGA: 18
HGNC: HGNC:4046
Homologene: 40606
Zfp106
Name: zinc finger protein 106
Synonyms: Cd-1, H3a, sirm, Sh3bp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20402
Homologene: 40787
Prmt3
Name: protein arginine N-methyltransferase 3
Synonyms: 2410018A17Rik, 2010005E20Rik, Hrmt1l3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71974
Homologene: 24255
Lpin2
Name: lipin 2
Synonyms: 2610511G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 64898
Homologene: 8769
Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21819
Homologene: 2430
Srprb
Name: signal recognition particle receptor, B subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20818
Homologene: 6332
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: 4930400K19Rik, Tulip1, 2310003F20Rik, Garnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Rims2
Name: regulating synaptic membrane exocytosis 2
Synonyms: RIM2, 2810036I15Rik, Syt3-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 116838
VEGA: 15
Homologene: 81639
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: chromatin remodeling factor, 8030453L17Rik, E430018P19Rik, KMT2H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 192195
Homologene: 10225
Tasor2
Name: transcription activation suppressor family member 2
Synonyms: BC016423, Fam208b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105203
VEGA: 13
Homologene: 26435
Car9
Name: carbonic anhydrase 9
Synonyms: CAIX, MN/CA9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230099
HGNC: HGNC:1383
Homologene: 20325
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: DHC1b, DHC2, 4432416O06Rik, D330044F14Rik, D030010H02Rik, Dnchc2, b2b414Clo, m407Asp, m152Asp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Snx9
Name: sorting nexin 9
Synonyms: SH3PX1, SDP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 66616
VEGA: 17
Homologene: 49454
Zfp608
Name: zinc finger protein 608
Synonyms: 4932417D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269023
VEGA: 18
Homologene: 18485
Plekhg4
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 4
Synonyms: 4931414L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102075
Homologene: 18516
Myo1c
Name: myosin IC
Synonyms: myosin-Ibeta, myr2, C80397, mm1beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17913
HGNC: HGNC:7597
Homologene: 32046
Cfap52
Name: cilia and flagella associated protein 52
Synonyms: 4933417B11Rik, 1700019F09Rik, Wdr16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71860
Homologene: 12416
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Il10
Name: interleukin 10
Synonyms: cytokine synthesis inhibitory factor, IL-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16153
HGNC: HGNC:5962
Homologene: 478
Nme8
Name: NME/NM23 family member 8
Synonyms: 1700056P15Rik, Sptrx-2, Txndc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 73412
Homologene: 9593
Pcdhb9
Name: protocadherin beta 9
Synonyms: Pcdhb4C, PcdhbI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93880
Homologene: 87124
Fam186a
Name: family with sequence similarity 186, member A
Synonyms: LOC380973, 1700030F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72277
Clcn4
Name: chloride channel, voltage-sensitive 4
Synonyms: Clc4-2, Clcn4-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12727
HGNC: HGNC:2022
Homologene: 68207
Aoah
Name: acyloxyacyl hydrolase
Synonyms: 4930433E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 27052
VEGA: 13
HGNC: HGNC:548
Homologene: 1238
Gnat2
Name: guanine nucleotide binding protein, alpha transducing 2
Synonyms: Gnat-2, Tcalpha, Gt-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 14686
HGNC: HGNC:4394
Homologene: 21092
Vmn2r8
Name: vomeronasal 2, receptor 8
Synonyms: EG627479
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 627479
Homologene: 129606
Lztr1
Name: leucine-zipper-like transcriptional regulator, 1
Synonyms: TCFL2, 1200003E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66863
HGNC: HGNC:6742
Homologene: 4925
Tm2d2
Name: TM2 domain containing 2
Synonyms: 2410018G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 69742
Homologene: 12328
Olfr1339
Name: olfactory receptor 1339
Synonyms: GA_x6K02T2QD9B-18815145-18814198, MOR258-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 258851
Homologene: 27280
Tmem132b
Name: transmembrane protein 132B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 208151
Homologene: 28135
Lmtk3
Name: lemur tyrosine kinase 3
Synonyms: Aatyk3, AATYK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381983
Homologene: 79449
Zscan5b
Name: zinc finger and SCAN domain containing 5B
Synonyms: Zfg1, Zfp371
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 170734
Homologene: 129555
Fhit
Name: fragile histidine triad gene
Synonyms: Fra14A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14198
HGNC: HGNC:3701
Homologene: 21661
Adad2
Name: adenosine deaminase domain containing 2
Synonyms: 4930403J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 75773
Homologene: 16392
Gsdmc4
Name: gasdermin C4
Synonyms: 9030605I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74548
HGNC: HGNC:7151
Homologene: 69487
Olfr1089
Name: olfactory receptor 1089
Synonyms: GA_x6K02T2Q125-48226321-48225386, MOR193-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 257933
Homologene: 79354
Bcas2
Name: breast carcinoma amplified sequence 2
Synonyms: 6430539P16Rik, C76366
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68183
HGNC: HGNC:975
Homologene: 4291
Tas2r119
Name: taste receptor, type 2, member 119
Synonyms: mt2r19, Tas2r19, mGR19, T2R19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 57254
Homologene: 10480
Hemk1
Name: HemK methyltransferase family member 1
Synonyms: 2310008M14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69536
Homologene: 6815
Slc6a16
Name: solute carrier family 6, member 16
Synonyms: LOC381884
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381884
Homologene: 49366
RP23-201D22.4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Gm2788
Name: predicted gene 2788
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108167326
Trav9-4
Name: T cell receptor alpha variable 9-4
Synonyms: Gm13907
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 630461
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 131,024,203 bp
  • T to C, chromosome 2 at 86,732,805 bp
  • C to G, chromosome 2 at 120,531,957 bp
  • G to A, chromosome 2 at 156,533,771 bp
  • T to A, chromosome 3 at 88,985,609 bp
  • T to C, chromosome 3 at 103,173,261 bp
  • C to T, chromosome 3 at 108,096,496 bp
  • T to A, chromosome 4 at 43,509,146 bp
  • T to A, chromosome 4 at 118,735,371 bp
  • T to C, chromosome 4 at 151,979,510 bp
  • A to G, chromosome 5 at 34,307,067 bp
  • A to T, chromosome 5 at 75,609,394 bp
  • T to A, chromosome 5 at 108,802,459 bp
  • A to G, chromosome 5 at 125,622,646 bp
  • A to G, chromosome 7 at 6,230,519 bp
  • C to A, chromosome 7 at 7,288,944 bp
  • A to G, chromosome 7 at 24,559,845 bp
  • T to G, chromosome 7 at 45,261,108 bp
  • C to A, chromosome 7 at 45,786,862 bp
  • G to T, chromosome 7 at 48,877,961 bp
  • T to A, chromosome 7 at 49,780,334 bp
  • T to C, chromosome 7 at 144,938,012 bp
  • A to G, chromosome 8 at 25,022,768 bp
  • T to C, chromosome 8 at 105,380,750 bp
  • T to A, chromosome 8 at 119,615,105 bp
  • C to T, chromosome 8 at 125,761,523 bp
  • A to G, chromosome 9 at 7,162,779 bp
  • A to G, chromosome 9 at 99,411,485 bp
  • A to G, chromosome 9 at 103,197,601 bp
  • T to C, chromosome 9 at 107,331,528 bp
  • G to A, chromosome 10 at 67,233,414 bp
  • C to G, chromosome 11 at 67,927,358 bp
  • A to G, chromosome 11 at 75,662,635 bp
  • T to C, chromosome 12 at 55,612,738 bp
  • A to G, chromosome 13 at 3,584,996 bp
  • A to C, chromosome 13 at 19,677,868 bp
  • A to T, chromosome 13 at 20,995,479 bp
  • A to G, chromosome 14 at 10,421,534 bp
  • T to C, chromosome 14 at 53,676,429 bp
  • A to T, chromosome 15 at 32,177,968 bp
  • A to T, chromosome 15 at 39,345,413 bp
  • A to G, chromosome 15 at 63,892,654 bp
  • A to T, chromosome 15 at 66,838,057 bp
  • A to C, chromosome 15 at 99,941,747 bp
  • A to G, chromosome 16 at 17,512,129 bp
  • T to A, chromosome 17 at 5,891,809 bp
  • T to A, chromosome 17 at 71,243,961 bp
  • G to A, chromosome 18 at 9,213,268 bp
  • A to T, chromosome 18 at 37,401,406 bp
  • A to T, chromosome 18 at 54,898,272 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5624 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043163-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.