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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR5870Btlr/Mmmh
Stock Number:
044078-MU
Citation ID:
RRID:MMRRC_044078-MU
Other Names:
R5870 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095

Additional Resources
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Adcy9
Name: adenylate cyclase 9
Synonyms: D16Wsu65e, ACtp10
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11515
HGNC: HGNC:240
Homologene: 868

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tm9sf4
Name: transmembrane 9 superfamily member 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99237
Homologene: 21620

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Fuz
Name: fuzzy planar cell polarity protein
Synonyms: 2600013E07Rik, b2b1273Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70300
Homologene: 11840

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Usp28
Name: ubiquitin specific peptidase 28
Synonyms: 9830148O20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235323
VEGA: 9
Homologene: 10840

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Synj2
Name: synaptojanin 2
Synonyms: SJ2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20975
Homologene: 117703

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Arid1a
Name: AT-rich interaction domain 1A
Synonyms: Osa1, 1110030E03Rik, Smarcf1, BAF250a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 93760
Homologene: 21216

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,180,938 bp
  • T to A, chromosome 1 at 150,455,549 bp
  • A to T, chromosome 1 at 191,985,300 bp
  • G to T, chromosome 2 at 26,916,259 bp
  • A to G, chromosome 2 at 27,220,641 bp
  • A to G, chromosome 2 at 29,389,811 bp
  • T to A, chromosome 2 at 76,872,714 bp
  • G to A, chromosome 2 at 131,072,847 bp
  • A to G, chromosome 2 at 153,194,281 bp
  • A to T, chromosome 3 at 84,690,521 bp
  • T to C, chromosome 3 at 104,785,495 bp
  • T to C, chromosome 4 at 99,063,962 bp
  • G to A, chromosome 4 at 107,066,722 bp
  • G to A, chromosome 4 at 109,361,310 bp
  • T to A, chromosome 4 at 127,195,709 bp
  • T to C, chromosome 4 at 133,681,076 bp
  • T to A, chromosome 4 at 140,826,581 bp
  • G to A, chromosome 5 at 30,144,286 bp
  • C to T, chromosome 5 at 31,276,940 bp
  • T to C, chromosome 5 at 57,720,411 bp
  • A to G, chromosome 5 at 127,677,280 bp
  • G to A, chromosome 6 at 25,778,951 bp
  • A to T, chromosome 6 at 30,382,683 bp
  • A to G, chromosome 6 at 41,084,914 bp
  • A to T, chromosome 6 at 58,916,450 bp
  • A to G, chromosome 6 at 72,594,738 bp
  • T to A, chromosome 7 at 24,756,332 bp
  • T to G, chromosome 7 at 24,997,578 bp
  • A to T, chromosome 7 at 28,392,497 bp
  • A to G, chromosome 7 at 44,900,318 bp
  • A to G, chromosome 7 at 103,155,741 bp
  • T to C, chromosome 7 at 114,031,786 bp
  • G to A, chromosome 7 at 126,978,922 bp
  • A to G, chromosome 8 at 4,235,725 bp
  • G to T, chromosome 8 at 90,908,392 bp
  • A to G, chromosome 9 at 20,260,578 bp
  • C to T, chromosome 9 at 49,025,985 bp
  • A to T, chromosome 9 at 82,908,677 bp
  • C to A, chromosome 9 at 86,570,361 bp
  • A to G, chromosome 9 at 120,167,853 bp
  • A to T, chromosome 10 at 79,612,209 bp
  • T to A, chromosome 11 at 60,203,584 bp
  • C to T, chromosome 11 at 66,085,210 bp
  • A to G, chromosome 11 at 67,201,979 bp
  • C to T, chromosome 11 at 69,678,222 bp
  • A to G, chromosome 11 at 76,022,038 bp
  • A to T, chromosome 11 at 90,537,956 bp
  • A to G, chromosome 11 at 116,214,925 bp
  • T to A, chromosome 11 at 116,347,074 bp
  • A to T, chromosome 12 at 101,652,852 bp
  • A to G, chromosome 12 at 116,256,245 bp
  • A to T, chromosome 13 at 61,355,731 bp
  • C to A, chromosome 13 at 100,655,424 bp
  • T to A, chromosome 14 at 39,472,629 bp
  • G to A, chromosome 14 at 79,609,011 bp
  • A to G, chromosome 14 at 118,267,377 bp
  • T to A, chromosome 15 at 12,160,615 bp
  • A to T, chromosome 15 at 74,763,532 bp
  • A to T, chromosome 15 at 83,577,036 bp
  • G to A, chromosome 15 at 98,543,513 bp
  • A to G, chromosome 16 at 4,418,368 bp
  • A to G, chromosome 16 at 94,123,334 bp
  • A to G, chromosome 17 at 6,037,853 bp
  • A to G, chromosome 17 at 22,619,023 bp
  • A to T, chromosome 18 at 10,593,744 bp
  • A to C, chromosome 18 at 15,399,889 bp
  • T to C, chromosome 18 at 53,409,261 bp
  • T to A, chromosome 18 at 80,408,686 bp
  • A to T, chromosome 18 at 82,406,072 bp
  • T to A, chromosome 19 at 4,149,385 bp
  • G to T, chromosome 19 at 25,132,126 bp
  • T to G, chromosome 19 at 42,171,671 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5870 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
044078-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.