Strain Name:
C57BL/6J-MtgxR6049Btlr
Stock Number:
044217-MU
Citation ID:
RRID:MMRRC_044217-MU
Other Names:
R6049 (G1)
Major Collection:

Gene Information

Gene Symbol: Pdpk1 [MGI:1338068] (Mus musculus (mouse))
Name: 3-phosphoinositide dependent protein kinase 1
Synonyms: Pdk1, Pkb kinase
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Limch1 [MGI:1924819] (Mus musculus (mouse))
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Slc1a3 [MGI:99917] (Mus musculus (mouse))
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: B430115D02Rik, Eaat1, Gmt1, GLAST, MGluT1
Chromosome: 15
Alteration at locus: Chemically Induced
Gene Symbol: Ank2 [MGI:88025] (Mus musculus (mouse))
Name: ankyrin 2, brain
Synonyms: Ankyrin-2, ankyrin B, Ankyrin-B, Gm4392, Ank-2
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Prkcd [MGI:97598] (Mus musculus (mouse))
Name: protein kinase C, delta
Synonyms: Pkcd, PKCdelta, D14Ertd420e, PKC[d]
Chromosome: 14
Alteration at locus: Chemically Induced
Gene Symbol: Rfx3 [MGI:106582] (Mus musculus (mouse))
Name: regulatory factor X, 3 (influences HLA class II expression)
Synonyms: MRFX3, C230093O12Rik
Chromosome: 19
Alteration at locus: Chemically Induced
Gene Symbol: Cemip2 [MGI:1890373] (Mus musculus (mouse))
Name: cell migration inducing hyaluronidase 2
Synonyms: 3110012M15Rik, Tmem2
Chromosome: 19
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 46,085,602 bp
  • T to C, chromosome 1 at 159,912,754 bp
  • T to C, chromosome 2 at 18,231,700 bp
  • T to C, chromosome 2 at 24,094,992 bp
  • A to G, chromosome 2 at 30,403,541 bp
  • G to A, chromosome 2 at 76,846,310 bp
  • A to G, chromosome 2 at 119,153,425 bp
  • G to T, chromosome 2 at 152,323,174 bp
  • A to C, chromosome 3 at 59,752,149 bp
  • A to G, chromosome 3 at 96,851,663 bp
  • T to A, chromosome 3 at 126,943,020 bp
  • A to G, chromosome 4 at 46,787,641 bp
  • T to C, chromosome 4 at 61,227,597 bp
  • G to A, chromosome 4 at 103,231,323 bp
  • A to G, chromosome 4 at 118,402,988 bp
  • G to T, chromosome 4 at 132,459,713 bp
  • T to A, chromosome 4 at 141,218,162 bp
  • A to T, chromosome 5 at 67,030,860 bp
  • C to T, chromosome 5 at 107,118,485 bp
  • T to C, chromosome 5 at 136,332,710 bp
  • A to G, chromosome 6 at 17,694,348 bp
  • T to C, chromosome 6 at 73,086,166 bp
  • A to C, chromosome 6 at 113,417,841 bp
  • T to A, chromosome 7 at 10,714,713 bp
  • A to G, chromosome 7 at 28,315,197 bp
  • A to T, chromosome 7 at 127,193,835 bp
  • T to C, chromosome 7 at 134,298,232 bp
  • A to C, chromosome 8 at 11,006,805 bp
  • T to A, chromosome 8 at 22,939,037 bp
  • A to G, chromosome 8 at 84,638,846 bp
  • T to C, chromosome 8 at 95,270,842 bp
  • A to G, chromosome 9 at 15,561,256 bp
  • C to A, chromosome 9 at 19,361,344 bp
  • G to A, chromosome 9 at 123,011,622 bp
  • A to T, chromosome 10 at 5,347,926 bp
  • A to G, chromosome 10 at 10,378,026 bp
  • A to T, chromosome 10 at 45,686,662 bp
  • A to T, chromosome 10 at 130,112,612 bp
  • A to G, chromosome 11 at 78,028,170 bp
  • C to T, chromosome 11 at 100,267,060 bp
  • G to A, chromosome 11 at 105,867,717 bp
  • T to A, chromosome 12 at 16,681,394 bp
  • T to C, chromosome 12 at 64,474,320 bp
  • T to C, chromosome 12 at 81,591,323 bp
  • G to T, chromosome 13 at 27,606,178 bp
  • A to C, chromosome 13 at 43,159,360 bp
  • A to G, chromosome 13 at 61,038,758 bp
  • A to G, chromosome 13 at 81,397,354 bp
  • A to T, chromosome 13 at 109,032,585 bp
  • T to C, chromosome 14 at 30,607,297 bp
  • T to C, chromosome 15 at 4,735,172 bp
  • T to C, chromosome 15 at 8,645,693 bp
  • A to G, chromosome 15 at 76,727,401 bp
  • A to G, chromosome 15 at 85,121,695 bp
  • A to G, chromosome 15 at 91,178,236 bp
  • A to T, chromosome 16 at 59,086,146 bp
  • T to C, chromosome 16 at 59,544,054 bp
  • A to T, chromosome 17 at 24,098,135 bp
  • T to A, chromosome 18 at 58,598,660 bp
  • T to C, chromosome 18 at 74,202,600 bp
  • A to T, chromosome 19 at 21,826,126 bp
  • A to T, chromosome 19 at 27,802,395 bp
  • A to G, chromosome 19 at 58,760,452 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6049 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044217-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.