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Strain Name:
C57BL/6J-MtgxR6005Btlr/Mmmh
Stock Number:
044425-MU
Citation ID:
RRID:MMRRC_044425-MU
Other Names:
R6005 (G1)
Major Collection:

Strain Information

Sptbn4
Name: spectrin beta, non-erythrocytic 4
Synonyms: dyn, SpbIV, neuroaxonal dystrophy, 5830426A08Rik, ROSA62, nmf261, 1700022P15Rik, Spnb4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80297
Homologene: 11879
Map2k5
Name: mitogen-activated protein kinase kinase 5
Synonyms: MEK5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23938
VEGA: 9
HGNC: HGNC:6845
Homologene: 115933
Wnt5b
Name: wingless-type MMTV integration site family, member 5B
Synonyms: Wnt-5b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22419
Homologene: 22530
Prss12
Name: serine protease 12 neurotrypsin (motopsin)
Synonyms: Bssp-3, motopsin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19142
HGNC: HGNC:9477
Homologene: 7490
Epb41l4a
Name: erythrocyte membrane protein band 4.1 like 4a
Synonyms: NBL4, Epb4.1l4a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13824
VEGA: 18
Homologene: 8398
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 4,890,773 bp
  • C to T, chromosome 1 at 66,627,257 bp
  • C to A, chromosome 1 at 75,492,215 bp
  • T to C, chromosome 1 at 91,114,611 bp
  • T to C, chromosome 1 at 106,734,581 bp
  • C to T, chromosome 1 at 118,842,064 bp
  • A to T, chromosome 1 at 163,075,677 bp
  • T to C, chromosome 2 at 9,882,827 bp
  • C to T, chromosome 2 at 37,428,965 bp
  • A to T, chromosome 2 at 39,001,321 bp
  • T to C, chromosome 2 at 70,690,751 bp
  • A to T, chromosome 2 at 76,769,563 bp
  • A to G, chromosome 2 at 87,108,063 bp
  • A to T, chromosome 2 at 87,608,080 bp
  • C to T, chromosome 2 at 101,696,684 bp
  • G to A, chromosome 2 at 158,262,480 bp
  • C to T, chromosome 3 at 88,412,846 bp
  • A to T, chromosome 3 at 96,719,468 bp
  • A to T, chromosome 3 at 123,482,768 bp
  • T to A, chromosome 4 at 41,498,895 bp
  • T to C, chromosome 4 at 41,763,887 bp
  • AGGGG to AGGG, chromosome 4 at 44,931,218 bp
  • T to A, chromosome 4 at 123,474,275 bp
  • T to C, chromosome 4 at 129,491,503 bp
  • C to A, chromosome 4 at 135,412,307 bp
  • T to A, chromosome 4 at 143,698,432 bp
  • T to C, chromosome 5 at 16,361,821 bp
  • G to T, chromosome 5 at 34,562,465 bp
  • A to G, chromosome 5 at 36,019,384 bp
  • C to T, chromosome 5 at 73,083,295 bp
  • T to C, chromosome 5 at 100,363,878 bp
  • G to A, chromosome 5 at 121,205,514 bp
  • G to T, chromosome 5 at 124,340,774 bp
  • C to A, chromosome 5 at 134,255,958 bp
  • A to G, chromosome 5 at 150,769,776 bp
  • G to A, chromosome 6 at 18,030,323 bp
  • A to T, chromosome 6 at 42,589,971 bp
  • A to G, chromosome 6 at 49,367,822 bp
  • A to T, chromosome 6 at 56,479,202 bp
  • G to A, chromosome 6 at 119,433,654 bp
  • A to C, chromosome 6 at 123,267,159 bp
  • G to A, chromosome 6 at 132,893,696 bp
  • C to T, chromosome 7 at 27,257,325 bp
  • A to G, chromosome 7 at 27,418,599 bp
  • A to T, chromosome 7 at 103,041,646 bp
  • G to T, chromosome 8 at 18,934,505 bp
  • A to G, chromosome 8 at 23,132,202 bp
  • A to G, chromosome 8 at 77,674,927 bp
  • T to C, chromosome 9 at 3,865,518 bp
  • C to T, chromosome 9 at 19,584,885 bp
  • T to C, chromosome 9 at 38,378,309 bp
  • G to A, chromosome 9 at 54,908,805 bp
  • C to T, chromosome 9 at 63,281,019 bp
  • T to G, chromosome 9 at 103,448,550 bp
  • T to A, chromosome 9 at 121,748,394 bp
  • C to T, chromosome 10 at 10,395,352 bp
  • T to A, chromosome 10 at 36,832,928 bp
  • G to A, chromosome 10 at 128,244,200 bp
  • A to C, chromosome 11 at 8,857,113 bp
  • A to G, chromosome 12 at 98,678,552 bp
  • A to G, chromosome 13 at 43,668,035 bp
  • C to A, chromosome 14 at 35,323,412 bp
  • C to T, chromosome 14 at 54,187,931 bp
  • A to C, chromosome 14 at 103,156,723 bp
  • T to A, chromosome 15 at 36,010,567 bp
  • A to G, chromosome 15 at 76,057,751 bp
  • G to A, chromosome 15 at 76,912,085 bp
  • A to T, chromosome 15 at 85,494,109 bp
  • T to C, chromosome 15 at 89,188,168 bp
  • T to C, chromosome 15 at 96,370,972 bp
  • A to G, chromosome 15 at 102,281,492 bp
  • C to T, chromosome 16 at 75,873,514 bp
  • A to T, chromosome 16 at 90,969,286 bp
  • C to G, chromosome 17 at 27,190,785 bp
  • T to A, chromosome 17 at 34,923,454 bp
  • C to T, chromosome 18 at 33,828,143 bp
  • T to A, chromosome 18 at 37,519,736 bp
  • C to T, chromosome 18 at 43,792,797 bp
  • A to T, chromosome 19 at 4,903,795 bp
  • A to G, chromosome 19 at 5,656,201 bp
  • T to C, chromosome 19 at 9,015,161 bp
  • A to G, chromosome 19 at 42,040,115 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6005 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044425-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.