Strain Name:
C57BL/6J-MtgxR6548Btlr/Mmmh
Stock Number:
044673-MU
Citation ID:
RRID:MMRRC_044673-MU
Other Names:
R6548 (G1)
Major Collection:

Strain Information

Tgfb1
Name: transforming growth factor, beta 1
Synonyms: TGF-beta1, TGF-beta 1, TGFbeta1, Tgfb, Tgfb-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21803
Homologene: 540
Sod2
Name: superoxide dismutase 2, mitochondrial
Synonyms: manganese SOD, manganese superoxide dismutase, Sod-2, MnSOD
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20656
VEGA: 17
Homologene: 530
Gpr37
Name: G protein-coupled receptor 37
Synonyms: parkin-associated endothelin B-like receptor, Pael-R
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14763
HGNC: HGNC:4494
Homologene: 3875
Net1
Name: neuroepithelial cell transforming gene 1
Synonyms: Net1 homolog, 9530071N24Rik, 0610025H04Rik, mNET1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56349
VEGA: 13
Homologene: 4283
Ubap2l
Name: ubiquitin-associated protein 2-like
Synonyms: NICE-4, 4932431F02Rik, A430103N23Rik, 3110083O19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74383
Homologene: 136291
Ints3
Name: integrator complex subunit 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229543
Homologene: 11309
Exoc2
Name: exocyst complex component 2
Synonyms: 2410030I24Rik, Sec5, Sec5l1, Gm29675
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66482
Homologene: 10122
Wdr7
Name: WD repeat domain 7
Synonyms: TRAG, TGF-beta resistance associated gene
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 104082
VEGA: 18
Homologene: 11408
Mon2
Name: MON2 homolog, regulator of endosome to Golgi trafficking
Synonyms: SF21, 2610528O22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67074
VEGA: 10
Homologene: 44309
Brca1
Name: breast cancer 1, early onset
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12189
HGNC: HGNC:1100
Homologene: 5276
Smurf1
Name: SMAD specific E3 ubiquitin protein ligase 1
Synonyms: 4930431E10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75788
Homologene: 10712
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, AnkG, 2900054D09Rik, Ankyrin-3, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Rasal1
Name: RAS protein activator like 1 (GAP1 like)
Synonyms: MRASAL
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19415
HGNC: HGNC:9873
Homologene: 3423
Platr25
Name: pluripotency associated transcript 25
Synonyms: Gm5665, 6720490N10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 435366
Lyar
Name: Ly1 antibody reactive clone
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17089
Homologene: 41200
Tln1
Name: talin 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21894
Homologene: 21267
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: 9330197E15Rik, A630028O16Rik, LOC380767
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 211978
VEGA: 12
Homologene: 9102
Or5p76
Name: olfactory receptor family 5 subfamily P member 76
Synonyms: GA_x6K02T2PBJ9-10853935-10852991, MOR204-8, Olfr502
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258734
Homologene: 72021
Chd3
Name: chromodomain helicase DNA binding protein 3
Synonyms: Prp9-1, Chd7, 2600010P09Rik, Mi-2 alpha, Prp7
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216848
HGNC: HGNC:1918
Homologene: 62693
Wdr24
Name: WD repeat domain 24
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268933
Homologene: 6870
Myh2
Name: myosin, heavy polypeptide 2, skeletal muscle, adult
Synonyms: Myhs-f, MyHC-IIa, MHC2A, Myhsf1, Myhs-f1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17882
HGNC: HGNC:7572
Homologene: 23019
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: b2b1304Clo, b2b2025.1Clo, b2b1735Clo, 4921507O14Rik, prh, D3Ertd789e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 51938
Homologene: 12149
Serpina1a
Name: serine (or cysteine) peptidase inhibitor, clade A, member 1A
Synonyms: Aat2, Spi1-1, PI1, Aat-2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20700
HGNC: HGNC:8941
Homologene: 20103
Topaz1
Name: testis and ovary specific PAZ domain containing 1
Synonyms: Gm9524
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 671232
VEGA: 9
Homologene: 19835
D630003M21Rik
Name: RIKEN cDNA D630003M21 gene
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228846
Homologene: 18598
Mug2
Name: murinoglobulin 2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17837
HGNC: HGNC:9750
Homologene: 136663
Serpina1d
Name: serine (or cysteine) peptidase inhibitor, clade A, member 1D
Synonyms: Spi1-4, PI4
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20703
HGNC: HGNC:8941
Homologene: 20103
Bap1
Name: Brca1 associated protein 1
Synonyms: 2300006C11Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 104416
HGNC: HGNC:950
Homologene: 3421
Or52s1
Name: olfactory receptor family 52 subfamily S member 1
Synonyms: Olfr593, MOR24-2, GA_x6K02T2PBJ9-5927412-5928362
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258378
Homologene: 27147
Ssbp2
Name: single-stranded DNA binding protein 2
Synonyms: 2310079I02Rik, Ssdp2, 1500004K09Rik, A830008M03Rik, Hspc116, 9330163K02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66970
Homologene: 22724
Fcgbp
Name: Fc fragment of IgG binding protein
Synonyms: A430096B05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 215384
Homologene: 68369
Vmn1r62
Name: vomeronasal 1 receptor 62
Synonyms: V1rd8, V1rd2, V3R2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 81016
Homologene: 41799
Plk5
Name: polo like kinase 5
Synonyms: 6330514A18Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216166
Homologene: 28072
Gpc2
Name: glypican 2 cerebroglycan
Synonyms: 2410016G05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71951
HGNC: HGNC:4450
Homologene: 17657
Champ1
Name: chromosome alignment maintaining phosphoprotein 1
Synonyms: D8Ertd569e, Zfp828, D8Ertd457e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 101994
Homologene: 18780
Tcl1b1
Name: T cell leukemia/lymphoma 1B, 1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 27379
VEGA: 12
Homologene: 83274
9030612E09Rik
Name: RIKEN cDNA 9030612E09 gene
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74530
VEGA: 10
Krt28
Name: keratin 28
Synonyms: 4733401L19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70843
Homologene: 124711
Lrrc75a
Name: leucine rich repeat containing 75A
Synonyms: BC046404, Fam211a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192976
Homologene: 65996
Gm10376
Name: predicted gene 10376
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100042130
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 158,205,699 bp
  • A to T, chromosome 3 at 33,837,959 bp
  • A to G, chromosome 3 at 90,023,560 bp
  • G to A, chromosome 3 at 90,392,124 bp
  • A to G, chromosome 4 at 43,547,525 bp
  • A to G, chromosome 5 at 38,227,858 bp
  • A to T, chromosome 5 at 120,674,725 bp
  • G to A, chromosome 5 at 138,277,271 bp
  • A to G, chromosome 5 at 144,899,497 bp
  • G to A, chromosome 6 at 25,688,813 bp
  • C to T, chromosome 6 at 122,047,442 bp
  • G to A, chromosome 7 at 5,675,770 bp
  • A to G, chromosome 7 at 25,696,925 bp
  • A to G, chromosome 7 at 28,091,918 bp
  • T to A, chromosome 7 at 103,211,904 bp
  • T to C, chromosome 7 at 108,523,216 bp
  • A to T, chromosome 8 at 13,880,002 bp
  • T to A, chromosome 9 at 122,748,354 bp
  • T to C, chromosome 10 at 43,174,773 bp
  • C to T, chromosome 10 at 69,892,410 bp
  • T to A, chromosome 10 at 80,363,045 bp
  • A to T, chromosome 10 at 123,036,093 bp
  • T to A, chromosome 11 at 62,606,095 bp
  • A to G, chromosome 11 at 67,186,612 bp
  • T to A, chromosome 11 at 69,362,060 bp
  • T to C, chromosome 11 at 99,367,013 bp
  • G to T, chromosome 11 at 101,524,765 bp
  • A to G, chromosome 12 at 79,238,335 bp
  • G to T, chromosome 12 at 103,767,552 bp
  • G to T, chromosome 12 at 103,853,758 bp
  • A to G, chromosome 12 at 105,164,404 bp
  • C to T, chromosome 13 at 3,886,074 bp
  • T to A, chromosome 13 at 11,668,821 bp
  • A to T, chromosome 13 at 30,826,064 bp
  • A to T, chromosome 13 at 62,673,809 bp
  • A to G, chromosome 13 at 91,539,351 bp
  • A to G, chromosome 14 at 31,256,225 bp
  • T to C, chromosome 14 at 43,015,568 bp
  • A to G, chromosome 17 at 13,008,363 bp
  • C to A, chromosome 17 at 25,827,925 bp
  • A to G, chromosome 18 at 63,778,251 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6548 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044673-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.