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Strain Name:
C57BL/6J-MtgxR6923Btlr/Mmmh
Stock Number:
045381-MU
Citation ID:
RRID:MMRRC_045381-MU
Other Names:
R6923 (G1)
Major Collection:

Strain Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Gpd2
Name: glycerol phosphate dehydrogenase 2, mitochondrial
Synonyms: Gdm1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14571
HGNC: HGNC:4456
Homologene: 352
Fkbp2
Name: FK506 binding protein 2
Synonyms: mFKBP13, mFKBP2, 13kDa
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14227
VEGA: 19
HGNC: HGNC:3718
Homologene: 40604
Cbfa2t2
Name: CBFA2/RUNX1 translocation partner 2
Synonyms: MTGR1, C330013D05Rik, Cbfa2t2h
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12396
HGNC: HGNC:1536
Homologene: 3733
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Senp2
Name: SUMO/sentrin specific peptidase 2
Synonyms: 4930538C18Rik, 2310007L05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 75826
VEGA: 16
Homologene: 11005
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 58,500,375 bp
  • A to T, chromosome 1 at 87,774,356 bp
  • A to T, chromosome 1 at 134,149,425 bp
  • T to C, chromosome 1 at 134,746,763 bp
  • A to G, chromosome 1 at 163,986,885 bp
  • A to C, chromosome 1 at 173,884,698 bp
  • A to G, chromosome 2 at 52,186,064 bp
  • G to A, chromosome 2 at 57,355,788 bp
  • C to T, chromosome 2 at 60,514,966 bp
  • A to G, chromosome 2 at 83,657,056 bp
  • G to A, chromosome 2 at 118,249,913 bp
  • C to T, chromosome 2 at 154,534,983 bp
  • T to A, chromosome 3 at 103,812,214 bp
  • C to T, chromosome 3 at 133,479,341 bp
  • T to C, chromosome 3 at 142,600,559 bp
  • T to C, chromosome 4 at 96,769,816 bp
  • G to T, chromosome 4 at 108,570,204 bp
  • C to T, chromosome 4 at 133,582,525 bp
  • A to T, chromosome 4 at 143,699,106 bp
  • A to G, chromosome 5 at 30,672,462 bp
  • A to G, chromosome 5 at 90,577,793 bp
  • A to G, chromosome 6 at 28,447,119 bp
  • A to T, chromosome 6 at 119,965,678 bp
  • T to G, chromosome 7 at 79,691,853 bp
  • C to A, chromosome 7 at 89,845,885 bp
  • T to A, chromosome 7 at 98,129,254 bp
  • C to T, chromosome 7 at 141,637,540 bp
  • A to G, chromosome 8 at 12,856,949 bp
  • G to A, chromosome 8 at 85,518,052 bp
  • A to G, chromosome 8 at 121,753,371 bp
  • T to C, chromosome 9 at 44,985,859 bp
  • T to A, chromosome 9 at 50,610,310 bp
  • T to G, chromosome 9 at 57,581,612 bp
  • T to A, chromosome 9 at 70,574,610 bp
  • T to C, chromosome 10 at 18,783,868 bp
  • C to A, chromosome 10 at 22,067,755 bp
  • T to A, chromosome 10 at 127,788,868 bp
  • G to A, chromosome 11 at 26,489,893 bp
  • C to T, chromosome 11 at 51,253,981 bp
  • C to T, chromosome 11 at 69,735,961 bp
  • T to A, chromosome 11 at 69,817,777 bp
  • C to G, chromosome 11 at 74,929,343 bp
  • T to A, chromosome 11 at 78,274,626 bp
  • A to T, chromosome 12 at 108,793,668 bp
  • T to A, chromosome 13 at 38,631,064 bp
  • T to A, chromosome 13 at 95,478,582 bp
  • G to T, chromosome 14 at 57,603,711 bp
  • T to G, chromosome 16 at 22,011,576 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • T to A, chromosome 17 at 47,786,983 bp
  • T to A, chromosome 17 at 58,092,350 bp
  • A to G, chromosome 17 at 78,492,914 bp
  • G to A, chromosome 17 at 91,088,233 bp
  • C to T, chromosome 18 at 37,342,469 bp
  • A to G, chromosome 19 at 6,979,169 bp
  • T to A, chromosome 19 at 12,675,045 bp
  • T to C, chromosome 19 at 12,901,312 bp
  • A to T, chromosome 19 at 25,095,606 bp
  • A to G, chromosome 19 at 46,450,966 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6923 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045381-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.