Strain Name:
C57BL/6J-MtgxR8168Btlr/Mmmh
Stock Number:
067594-MU
Citation ID:
RRID:MMRRC_067594-MU
Other Names:
R8168 (G1)
Major Collection:

Strain Information

Mtrr
Name: 5-methyltetrahydrofolate-homocysteine methyltransferase reductase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 210009
VEGA: 13
HGNC: HGNC:7473
Homologene: 11419
Dock5
Name: dedicator of cytokinesis 5
Synonyms: rlc, 1110060D06Rik, lr2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Mef2c
Name: myocyte enhancer factor 2C
Synonyms: 9930028G15Rik, 5430401D19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17260
HGNC: HGNC:6996
Homologene: 31087
Myo18a
Name: myosin XVIIIA
Synonyms: MyoPDZ
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 360013
Homologene: 7977
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Tshr
Name: thyroid stimulating hormone receptor
Synonyms: hyt, hypothroid, pet
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22095
Homologene: 315
Atp6v1b2
Name: ATPase, H+ transporting, lysosomal V1 subunit B2
Synonyms: Atp6b2, HO57
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11966
HGNC: HGNC:854
Homologene: 1279
Flrt1
Name: fibronectin leucine rich transmembrane protein 1
Synonyms: D630040I23Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 396184
VEGA: 19
HGNC: HGNC:3760
Homologene: 40864
Arhgef1
Name: Rho guanine nucleotide exchange factor 1
Synonyms: Lbcl2, Lsc
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16801
HGNC: HGNC:681
Homologene: 3454
Rcc1
Name: regulator of chromosome condensation 1
Synonyms: 4931417M11Rik, Chc1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100088
HGNC: HGNC:1913
Homologene: 55567
Htt
Name: huntingtin
Synonyms: HD, IT15, C430023I11Rik, htt, Hdh, huntingtin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15194
HGNC: HGNC:4851
Homologene: 1593
Nelfa
Name: negative elongation factor complex member A, Whsc2
Synonyms: Whsc2h, Nelf-A, Whsc2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 24116
Homologene: 68478
Gzf1
Name: GDNF-inducible zinc finger protein 1
Synonyms: 8430437G08Rik, Zfp336
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74533
Homologene: 11200
Raph1
Name: Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Synonyms: 9430025M21Rik, C730009O10Rik, Lpd, lamellipodin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 77300
Homologene: 71030
Cwc22
Name: CWC22 spliceosome-associated protein
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 80744
Homologene: 6048
Phaf1
Name: phagosome assembly factor 1
Synonyms: Lin10, D230025D16Rik, Mytho
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234678
Homologene: 11864
Vps13a
Name: vacuolar protein sorting 13A
Synonyms: 4930543C13Rik, D330038K10Rik, 4930516E05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 271564
VEGA: 19
HGNC: HGNC:1908
Homologene: 22068
Prdm10
Name: PR domain containing 10
Synonyms: LOC382066, tristanin
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382066
Homologene: 45718
Srcap
Name: Snf2-related CREBBP activator protein
Synonyms: D030022P06Rik, F630004O05Rik, B930091H02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043597
Homologene: 38213
Nlrc4
Name: NLR family, CARD domain containing 4
Synonyms: Ipaf, Card12, 9530011P19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268973
VEGA: 17
Homologene: 10924
Ptp4a3
Name: protein tyrosine phosphatase 4a3
Synonyms: Prl-3
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 19245
VEGA: 15
HGNC: HGNC:9636
Homologene: 22500
Ano4
Name: anoctamin 4
Synonyms: A330096O15Rik, Tmem16d
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320091
Homologene: 66599
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Prg4
Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)
Synonyms: SZP, MSF, DOL54, lubricin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 96875
HGNC: HGNC:9364
Homologene: 137352
Gnptab
Name: N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits
Synonyms: EG432486
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 432486
Homologene: 32576
Spag17
Name: sperm associated antigen 17
Synonyms: PF6, 4931427F14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74362
Homologene: 52601
Cfap410
Name: cilia and flagella associated protein 410
Synonyms: 1810043G02Rik, D10Jhu13e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67884
HGNC: HGNC:1260
Homologene: 3619
Mrgprb2
Name: MAS-related GPR, member B2
Synonyms: 4833406I20Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243979
Homologene: 86246
Foxf1
Name: forkhead box F1
Synonyms: Foxf1a, FREAC1, Freac-1, HFH-8, Foxf1, Hfh8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15227
HGNC: HGNC:3809
Homologene: 1114
Or9m1
Name: olfactory receptor family 9 subfamily M member 1
Synonyms: Olfr1154, GA_x6K02T2Q125-49403456-49402524, MOR173-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258641
Homologene: 74192
Vmn1r174
Name: vomeronasal 1 receptor 174
Synonyms: V1rd22
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 404291
Homologene: 79577
Or4p8
Name: olfactory receptor family 4 subfamily P member 8
Synonyms: MOR225-4, GA_x6K02T2Q125-50372411-50371485, Olfr1208
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258774
Homologene: 74190
H2-Aa
Name: histocompatibility 2, class II antigen A, alpha
Synonyms: I-Aalpha, Ia-1, Aalpha, H-2Aa, A alpha, Ia1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14960
Homologene: 123820
Cfap54
Name: cilia and flagella associated protein 54
Synonyms: 4930485B16Rik, Gm872, LOC380653
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 380654
Homologene: 133438
Nim1k
Name: NIM1 serine/threonine protein kinase
Synonyms: Nim1, E130304F04Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 245269
VEGA: 13
Homologene: 25286
9430020K01Rik
Name:
Type: Gene
Species: Mouse
Chromosome: 18
AI607873
Name:
Type: Gene
Species: Mouse
Chromosome: 1
Hdgfrp2
Name:
Type: Gene
Species: Mouse
Chromosome: 17
Tex40
Name:
Type: Gene
Species: Mouse
Chromosome: 19
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,499,620 bp
  • T to A, chromosome 1 at 150,455,850 bp
  • A to T, chromosome 1 at 172,099,672 bp
  • A to C, chromosome 1 at 173,729,938 bp
  • A to C, chromosome 2 at 77,927,271 bp
  • T to A, chromosome 2 at 87,903,199 bp
  • T to A, chromosome 2 at 88,896,776 bp
  • C to T, chromosome 2 at 148,684,766 bp
  • C to T, chromosome 3 at 100,034,984 bp
  • T to C, chromosome 4 at 132,335,785 bp
  • A to T, chromosome 5 at 33,921,907 bp
  • A to G, chromosome 5 at 34,882,956 bp
  • A to T, chromosome 7 at 23,754,671 bp
  • A to G, chromosome 7 at 24,925,406 bp
  • A to T, chromosome 7 at 48,552,019 bp
  • T to A, chromosome 7 at 127,542,523 bp
  • T to C, chromosome 8 at 69,108,331 bp
  • C to T, chromosome 8 at 105,248,769 bp
  • T to C, chromosome 8 at 121,085,162 bp
  • G to A, chromosome 9 at 31,346,967 bp
  • G to A, chromosome 10 at 77,982,944 bp
  • G to T, chromosome 10 at 88,419,133 bp
  • A to G, chromosome 10 at 88,980,995 bp
  • A to T, chromosome 10 at 92,908,877 bp
  • T to A, chromosome 11 at 77,821,142 bp
  • T to A, chromosome 12 at 91,511,965 bp
  • C to T, chromosome 13 at 68,572,613 bp
  • G to T, chromosome 13 at 83,656,350 bp
  • A to T, chromosome 13 at 119,712,752 bp
  • A to T, chromosome 14 at 67,770,197 bp
  • T to G, chromosome 15 at 73,756,846 bp
  • T to A, chromosome 17 at 34,287,721 bp
  • T to C, chromosome 17 at 56,082,282 bp
  • T to C, chromosome 17 at 74,445,211 bp
  • A to T, chromosome 18 at 4,675,094 bp
  • T to A, chromosome 18 at 12,506,942 bp
  • A to G, chromosome 19 at 6,922,652 bp
  • G to A, chromosome 19 at 7,096,637 bp
  • A to C, chromosome 19 at 16,749,548 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8168 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067594-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.