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Strain Name:
C57BL/6J-MtgxR8321Btlr/Mmmh
Stock Number:
067723-MU
Citation ID:
RRID:MMRRC_067723-MU
Other Names:
R8321 (G1)
Major Collection:

Strain Information

Foxq1
Name: forkhead box Q1
Synonyms: HFH-1, Hfh1l, Hfh1, sa
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15220
Homologene: 7359
Cdh11
Name: cadherin 11
Synonyms: osteoblast-cadherin, OB-cadherin, Cad11
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12552
HGNC: HGNC:1750
Homologene: 1361
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Gldc
Name: glycine decarboxylase
Synonyms: D19Wsu57e, D030049L12Rik, b2b2679Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Gnb1
Name: guanine nucleotide binding protein (G protein), beta 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14688
HGNC: HGNC:4396
Homologene: 55532
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 63,747,979 bp
  • T to A, chromosome 1 at 73,985,780 bp
  • G to A, chromosome 1 at 75,185,698 bp
  • G to A, chromosome 1 at 93,320,655 bp
  • A to T, chromosome 2 at 6,391,089 bp
  • A to T, chromosome 2 at 164,393,287 bp
  • A to G, chromosome 2 at 181,587,138 bp
  • T to A, chromosome 3 at 5,401,127 bp
  • T to A, chromosome 3 at 46,446,294 bp
  • G to A, chromosome 3 at 56,183,097 bp
  • C to T, chromosome 3 at 67,430,261 bp
  • A to G, chromosome 3 at 86,093,764 bp
  • T to C, chromosome 3 at 118,781,924 bp
  • T to A, chromosome 3 at 136,234,634 bp
  • T to A, chromosome 4 at 48,283,267 bp
  • A to G, chromosome 4 at 96,553,447 bp
  • A to G, chromosome 4 at 154,160,403 bp
  • A to T, chromosome 4 at 155,555,025 bp
  • A to T, chromosome 5 at 48,230,267 bp
  • C to T, chromosome 5 at 120,666,355 bp
  • A to T, chromosome 5 at 138,757,931 bp
  • A to G, chromosome 6 at 28,242,993 bp
  • C to T, chromosome 6 at 58,843,769 bp
  • GA to GAA, chromosome 6 at 67,556,170 bp
  • A to G, chromosome 7 at 12,903,698 bp
  • A to G, chromosome 7 at 39,410,849 bp
  • C to T, chromosome 7 at 56,229,348 bp
  • TCCCAGG to T, chromosome 7 at 80,098,331 bp
  • C to T, chromosome 7 at 122,173,208 bp
  • C to A, chromosome 7 at 126,958,416 bp
  • T to C, chromosome 7 at 127,540,896 bp
  • T to C, chromosome 7 at 139,026,884 bp
  • C to T, chromosome 8 at 25,971,151 bp
  • G to A, chromosome 8 at 71,988,583 bp
  • T to C, chromosome 8 at 102,634,784 bp
  • A to T, chromosome 8 at 123,248,805 bp
  • A to T, chromosome 8 at 124,903,255 bp
  • G to T, chromosome 9 at 35,909,453 bp
  • A to T, chromosome 9 at 38,430,554 bp
  • C to A, chromosome 9 at 42,373,053 bp
  • C to A, chromosome 10 at 41,634,033 bp
  • T to C, chromosome 10 at 59,428,160 bp
  • A to G, chromosome 11 at 65,008,227 bp
  • G to A, chromosome 11 at 73,665,536 bp
  • G to A, chromosome 12 at 83,774,941 bp
  • T to C, chromosome 13 at 31,559,268 bp
  • T to A, chromosome 13 at 44,848,386 bp
  • C to T, chromosome 13 at 97,162,992 bp
  • T to C, chromosome 14 at 52,232,567 bp
  • G to T, chromosome 14 at 53,616,383 bp
  • TG to T, chromosome 14 at 56,424,542 bp
  • T to C, chromosome 15 at 27,881,326 bp
  • A to G, chromosome 16 at 59,915,954 bp
  • A to C, chromosome 16 at 89,031,736 bp
  • G to A, chromosome 17 at 25,720,891 bp
  • G to A, chromosome 19 at 30,143,407 bp
  • A to G, chromosome 19 at 38,651,936 bp
  • A to G, chromosome 19 at 47,958,147 bp
  • G to A, chromosome X at 102,908,615 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8321 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067723-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.