Strain Name:
C57BL/6J-MtgxR8321Btlr/Mmmh
Stock Number:
067723-MU
Citation ID:
RRID:MMRRC_067723-MU
Other Names:
R8321 (G1)
Major Collection:

Strain Information

Foxq1
Name: forkhead box Q1
Synonyms: HFH-1, Hfh1, Hfh1l, sa
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15220
Homologene: 7359
Cdh11
Name: cadherin 11
Synonyms: osteoblast-cadherin, Cad11, OB-cadherin
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12552
HGNC: HGNC:1750
Homologene: 1361
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Gldc
Name: glycine decarboxylase
Synonyms: D030049L12Rik, b2b2679Clo, D19Wsu57e
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Nbea
Name: neurobeachin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 26422
HGNC: HGNC:7648
Homologene: 69190
Gnb1
Name: guanine nucleotide binding protein (G protein), beta 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14688
HGNC: HGNC:4396
Homologene: 55532
Herc2
Name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Synonyms: D7H15F37S1, D7H15F32S1, rjs, jdf2, D15F32S1h
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 15204
HGNC: HGNC:4868
Homologene: 3430
Sh3d19
Name: SH3 domain protein D19
Synonyms: Kryn
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 27059
Homologene: 19064
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: Duplin, 5830451P18Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67772
Homologene: 72405
Gfm2
Name: G elongation factor, mitochondrial 2
Synonyms: EFG2, A930009M04Rik, MST027, 6530419G12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 320806
Homologene: 6238
Rnf17
Name: ring finger protein 17
Synonyms: MMIP-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 30054
VEGA: 14
Homologene: 23727
Trio
Name: triple functional domain (PTPRF interacting)
Synonyms: 6720464I07Rik, Solo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223435
VEGA: 15
Homologene: 20847
Rasal1
Name: RAS protein activator like 1 (GAP1 like)
Synonyms: MRASAL
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19415
HGNC: HGNC:9873
Homologene: 3423
Sez6l2
Name: seizure related 6 homolog like 2
Synonyms: Psk1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233878
Homologene: 8237
Slit2
Name: slit guidance ligand 2
Synonyms: Drad-1, E130320P19Rik, E030015M03Rik, Slil3, b2b1200.1Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20563
Homologene: 3516
Epha6
Name: Eph receptor A6
Synonyms: m-ehk2, Hek12, Ehk2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13840
Homologene: 56396
Oit3
Name: oncoprotein induced transcript 3
Synonyms: EF-9
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18302
Homologene: 7870
Usp6nl
Name: USP6 N-terminal like
Synonyms: TRE2NL
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98910
Homologene: 6879
Arhgap44
Name: Rho GTPase activating protein 44
Synonyms: AU040829
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216831
Homologene: 65284
Pask
Name: PAS domain containing serine/threonine kinase
Synonyms: Paskin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269224
Homologene: 9038
Zscan22
Name: zinc finger and SCAN domain containing 22
Synonyms: Hkr2, D530006B18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232878
HGNC: HGNC:4929
Homologene: 68122
Papln
Name: papilin, proteoglycan-like sulfated glycoprotein
Synonyms: E030033C16Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 170721
Homologene: 71541
Srcap
Name: Snf2-related CREBBP activator protein
Synonyms: D030022P06Rik, F630004O05Rik, B930091H02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043597
Homologene: 38213
Invs
Name: inversin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16348
Homologene: 7786
Cyp4f18
Name: cytochrome P450, family 4, subfamily f, polypeptide 18
Synonyms: 1810054N16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72054
Homologene: 128623
Ern2
Name: endoplasmic reticulum to nucleus signalling 2
Synonyms: Ire1b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26918
Homologene: 22687
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: IDPm, Idh-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Fastkd2
Name: FAST kinase domains 2
Synonyms: 2810421I24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75619
Homologene: 8957
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh4, Zfh-4, C130041O22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80892
Homologene: 23477
Hgsnat
Name: heparan-alpha-glucosaminide N-acetyltransferase
Synonyms: Tmem76, 9430010M12Rik, D8Ertd354e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 52120
Homologene: 15586
Tecta
Name: tectorin alpha
Synonyms: Tctna, [a]-tectorin
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21683
Homologene: 3955
Or8b42
Name: olfactory receptor family 8 subfamily B member 42
Synonyms: GA_x6K02T2PVTD-32123032-32123967, MOR162-8, Olfr901
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258028
VEGA: 9
Homologene: 79399
Dpyd
Name: dihydropyrimidine dehydrogenase
Synonyms: E330028L06Rik, DPD
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99586
HGNC: HGNC:3012
Homologene: 85
Cfap58
Name: cilia and flagella associated protein 58
Synonyms: Ccdc147, LOC381229
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 381229
VEGA: 19
Homologene: 77312
Tprg1l
Name: transformation related protein 63 regulated 1 like
Synonyms: Tprgl, mover, 1200015A19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67808
Homologene: 12192
Gm5114
Name: predicted gene 5114
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330513
Homologene: 45597
Chtf18
Name: CTF18, chromosome transmission fidelity factor 18
Synonyms: CTF18, 6030457M03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 214901
Homologene: 32532
Atg9a
Name: autophagy related 9A
Synonyms: Apg9l1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 245860
Homologene: 34495
Plce1
Name: phospholipase C, epsilon 1
Synonyms: PLCepsilon, 4933403A21Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74055
Homologene: 9478
Tns1
Name: tensin 1
Synonyms: Tns, E030018G17Rik, E030037J05Rik, 1110018I21Rik, 1200014E20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21961
Homologene: 11219
Cyp2j6
Name: cytochrome P450, family 2, subfamily j, polypeptide 6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13110
HGNC: HGNC:2634
Homologene: 68091
Zfp800
Name: zinc finger protein 800
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 627049
Homologene: 18529
Bank1
Name: B cell scaffold protein with ankyrin repeats 1
Synonyms: A530094C12Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242248
Homologene: 9926
Fam20c
Name: FAM20C, golgi associated secretory pathway kinase
Synonyms: DMP4
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80752
Homologene: 56879
Gfm1
Name: G elongation factor, mitochondrial 1
Synonyms: D3Wsu133e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 28030
Homologene: 6449
Zfp512b
Name: zinc finger protein 512B
Synonyms: LOC269401, Znf512b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269401
Homologene: 69314
Vps9d1
Name: VPS9 domain containing 1
Synonyms: 1300018I17Rik, 2410004N05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72325
Homologene: 21021
Sprtn
Name: SprT-like N-terminal domain
Synonyms: LOC244666, Gm505
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244666
Homologene: 32764
Pabpc4l
Name: poly(A) binding protein, cytoplasmic 4-like
Synonyms: EG241989, C330050A14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 241989
Homologene: 66336
Jakmip3
Name: janus kinase and microtubule interacting protein 3
Synonyms: 6330417G02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74004
Homologene: 90425
Pate13
Name: prostate and testis expressed 13
Synonyms: 9230113P08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77908
Matn4
Name: matrilin 4
Synonyms: matrilin-4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17183
HGNC: HGNC:6910
Homologene: 2844
Krtap6-1
Name: keratin associated protein 6-1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16700
VEGA: 16
Trav12-2
Name: T cell receptor alpha variable 12-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100043322
Igkv2-137
Name: immunoglobulin kappa chain variable 2-137
Synonyms: Gm16955
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 692187
Gm29096
Name: predicted gene 29096
Type: Gene
Species: Mouse
Chromosome: 10
Or1e27-ps1
Name: olfactory receptor family 1 subfamily E member 27, pseudogene 1
Synonyms: Olfr387-ps1, GA_x6K02T2P1NL-3823071-3824002, MOR135-17
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258210
Dmrtc1a
Name: DMRT-like family C1a
Synonyms: 1700030H14Rik, 4921520P21Rik, Dmrt8.1
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 70887
Homologene: 134338
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 63,747,979 bp
  • T to A, chromosome 1 at 73,985,780 bp
  • G to A, chromosome 1 at 75,185,698 bp
  • G to A, chromosome 1 at 93,320,655 bp
  • A to T, chromosome 2 at 6,391,089 bp
  • A to T, chromosome 2 at 164,393,287 bp
  • A to G, chromosome 2 at 181,587,138 bp
  • T to A, chromosome 3 at 5,401,127 bp
  • T to A, chromosome 3 at 46,446,294 bp
  • G to A, chromosome 3 at 56,183,097 bp
  • C to T, chromosome 3 at 67,430,261 bp
  • A to G, chromosome 3 at 86,093,764 bp
  • T to C, chromosome 3 at 118,781,924 bp
  • T to A, chromosome 3 at 136,234,634 bp
  • T to A, chromosome 4 at 48,283,267 bp
  • A to G, chromosome 4 at 96,553,447 bp
  • A to G, chromosome 4 at 154,160,403 bp
  • A to T, chromosome 4 at 155,555,025 bp
  • A to T, chromosome 5 at 48,230,267 bp
  • C to T, chromosome 5 at 120,666,355 bp
  • A to T, chromosome 5 at 138,757,931 bp
  • A to G, chromosome 6 at 28,242,993 bp
  • C to T, chromosome 6 at 58,843,769 bp
  • GA to GAA, chromosome 6 at 67,556,170 bp
  • A to G, chromosome 7 at 12,903,698 bp
  • A to G, chromosome 7 at 39,410,849 bp
  • C to T, chromosome 7 at 56,229,348 bp
  • TCCCAGG to T, chromosome 7 at 80,098,331 bp
  • C to T, chromosome 7 at 122,173,208 bp
  • C to A, chromosome 7 at 126,958,416 bp
  • T to C, chromosome 7 at 127,540,896 bp
  • T to C, chromosome 7 at 139,026,884 bp
  • C to T, chromosome 8 at 25,971,151 bp
  • G to A, chromosome 8 at 71,988,583 bp
  • T to C, chromosome 8 at 102,634,784 bp
  • A to T, chromosome 8 at 123,248,805 bp
  • A to T, chromosome 8 at 124,903,255 bp
  • G to T, chromosome 9 at 35,909,453 bp
  • A to T, chromosome 9 at 38,430,554 bp
  • C to A, chromosome 9 at 42,373,053 bp
  • C to A, chromosome 10 at 41,634,033 bp
  • T to C, chromosome 10 at 59,428,160 bp
  • A to G, chromosome 11 at 65,008,227 bp
  • G to A, chromosome 11 at 73,665,536 bp
  • G to A, chromosome 12 at 83,774,941 bp
  • T to C, chromosome 13 at 31,559,268 bp
  • T to A, chromosome 13 at 44,848,386 bp
  • C to T, chromosome 13 at 97,162,992 bp
  • T to C, chromosome 14 at 52,232,567 bp
  • G to T, chromosome 14 at 53,616,383 bp
  • TG to T, chromosome 14 at 56,424,542 bp
  • T to C, chromosome 15 at 27,881,326 bp
  • A to G, chromosome 16 at 59,915,954 bp
  • A to C, chromosome 16 at 89,031,736 bp
  • G to A, chromosome 17 at 25,720,891 bp
  • G to A, chromosome 19 at 30,143,407 bp
  • A to G, chromosome 19 at 38,651,936 bp
  • A to G, chromosome 19 at 47,958,147 bp
  • G to A, chromosome X at 102,908,615 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8321 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067723-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.