Strain Name:
B10.Cg-H2k2 H2-T18a Tg(SV40-HA)HA104Ajca/VradMmjax
Stock Number:
068214-JAX
Citation ID:
RRID:MMRRC_068214-JAX
Other Names:
B10.BR-HA104

Strain Information

H2-T18a
Name: histocompatibility 2, T region locus 18; a variant
Synonyms: Tlaa
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Non-mutant
H2k2
Name: histocompatibility-2, MHC; k2 variant
Synonyms: H2k
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Non-mutant
H2
Name: histocompatibility-2, MHC
Synonyms: H-2, MHC-II
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Non-mutant
H2-T18
Name: histocompatibility 2, T region locus 18
Synonyms: Tla, H-2T18, TL Ag
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Non-mutant
SV40
Name: simian virus 40 early region promoter/enhancer
Type: DNA Segment
Species: Simian virus 40 (SV40)
Chromosome:
Alteration at locus: Non-mutant
HA
Name: haemagglutinin, Influenza A virus (A/Puerto Rico/8/1934(H1N1))
Type: Gene
Species: Influenza virus
Chromosome:
Alteration at locus: Non-mutant
Tg(SV40-HA)HA104Ajca
Name: transgene insertion HA104, Andrew Caton
Synonyms: HA104
Type: Transgene
Species: Influenza virus
Chromosome: random insertion
Alteration at locus: Transgenic
Genetic Alterations
The transgenic construct contains the entire influenza virus A/Puerto Rico/8/34 H1N1(PR8) hemagglutinin (HA) under the direction of the SV40 early region promoter/enhancer. Founder line HA104 expresses low levels of transgene mRNA in several tissues, including the intestine, thymus, bone marrow, and spleen.

Due to backcrossing to the "B10.BR" line, the line holds additional alleles, H2k2 and H2-T18a:

The H2k2 haplotype differs from the prototype H2k in having the a, rather than the canonical b, alleles of the Class Ib genes T18 (formerly Tla) and Qa-1. The H2k2 haplotype is shared by the inbred strains C57BR/cdJ and C58/J and the H2-congenic strain B10.BR-H2k2 H2-T18a/SgSnJ (formerly B10.BR-H2k/SgSnJ).

The allele H2-T18a determines the presence of the antigenic specificities TL.1, 2, 3, 5, 6, 7 and occurs in strains A, C57BR, C58, SJL, and others.
ES Cell Line
Not applicable
Phenotype
None/Normal/Wild-type
Strain Development

The strain was developed by crossing C.Cg-Tg(SV40-HA)HA104Ajca/Mmjax (MMRRC:43831) to B10.BR-H2k2 H2-T18a/SgSnJJrep (RRID:IMSR_JAX:004804) for 9 generations.

Suggested Control Mice

B10.BR (RRID:IMSR_JAX:004804)

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Vedran Radojcic, M.D., University of Utah Huntsman Cancer Institute.
Primary Reference
Publication neither planned nor in preparation

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation

N9 (B10.BR-H2k2 H2-T18a/SgSnJJrep - RRID:IMSR_JAX:004804)

Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Eric Paulsen.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Eric Paulsen

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068214-JAX-M41-F
068214-JAX- M41-M
068214-JAX- M45-F
068214-JAX- M45-M
Homozygous for H2k2 Homozygous for H2-T18a Hemizygous for Tg(SV40-HA)HA104Ajca
Homozygous for H2k2 Homozygous for H2-T18a Hemizygous for Tg(SV40-HA)HA104Ajca
Homozygous for H2k2 Homozygous for H2-T18a Noncarrier for Tg(SV40-HA)HA104Ajca
Homozygous for H2k2 Homozygous for H2-T18a Noncarrier for Tg(SV40-HA)HA104Ajca
$218.00 / $218.00
Non-Profit / For-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
068214-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.