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Strain Name:
C57BL/6J-MtgxR8994Btlr/Mmmh
Stock Number:
068825-MU
Citation ID:
RRID:MMRRC_068825-MU
Other Names:
R8994 (G1)
Major Collection:

Strain Information

Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Med13l
Name: mediator complex subunit 13-like
Synonyms: 2210413I17Rik, 6330591G05Rik, Trap240L, 9030618F05Rik, Thrap2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76199
Homologene: 25256
Gpbp1
Name: GC-rich promoter binding protein 1
Synonyms: 1700034P14Rik, D230035M11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 73274
Homologene: 11292
Slain2
Name: SLAIN motif family, member 2
Synonyms: 8030444K12Rik, 5033405K12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75991
Homologene: 18952
Kif1b
Name: kinesin family member 1B
Synonyms: D4Mil1e, Kif1b alpha, Kif1b beta, KIF1Bp130, KIF1Bp204, N-3 kinesin, A530096N05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16561
Homologene: 99835
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 24,028,540 bp
  • T to C, chromosome 1 at 36,815,457 bp
  • T to A, chromosome 1 at 85,621,882 bp
  • G to A, chromosome 1 at 93,055,735 bp
  • C to A, chromosome 1 at 95,313,964 bp
  • G to T, chromosome 2 at 30,407,875 bp
  • C to T, chromosome 2 at 168,268,848 bp
  • A to T, chromosome 3 at 126,929,822 bp
  • C to A, chromosome 4 at 40,205,941 bp
  • T to C, chromosome 4 at 74,170,748 bp
  • T to C, chromosome 4 at 149,195,482 bp
  • T to A, chromosome 5 at 3,590,795 bp
  • T to A, chromosome 5 at 72,957,641 bp
  • A to C, chromosome 5 at 118,728,161 bp
  • T to A, chromosome 5 at 121,303,566 bp
  • A to G, chromosome 6 at 58,874,343 bp
  • C to A, chromosome 6 at 134,541,693 bp
  • A to G, chromosome 7 at 4,629,707 bp
  • G to T, chromosome 7 at 19,368,770 bp
  • A to C, chromosome 7 at 24,126,065 bp
  • A to T, chromosome 7 at 43,772,061 bp
  • A to G, chromosome 7 at 44,055,712 bp
  • G to T, chromosome 7 at 44,496,918 bp
  • A to T, chromosome 7 at 103,492,593 bp
  • G to A, chromosome 7 at 108,667,962 bp
  • A to G, chromosome 7 at 129,379,433 bp
  • T to C, chromosome 8 at 122,483,090 bp
  • A to T, chromosome 9 at 38,309,285 bp
  • T to C, chromosome 10 at 53,548,524 bp
  • C to A, chromosome 10 at 58,480,069 bp
  • A to T, chromosome 10 at 129,608,136 bp
  • A to G, chromosome 11 at 40,734,035 bp
  • A to T, chromosome 11 at 115,055,522 bp
  • A to T, chromosome 12 at 17,352,716 bp
  • A to G, chromosome 13 at 27,315,434 bp
  • T to A, chromosome 13 at 36,204,866 bp
  • T to C, chromosome 13 at 64,062,881 bp
  • T to C, chromosome 13 at 67,207,414 bp
  • GTT to GT, chromosome 13 at 81,405,338 bp
  • C to A, chromosome 13 at 111,466,850 bp
  • A to T, chromosome 14 at 36,940,119 bp
  • A to C, chromosome 14 at 111,680,795 bp
  • T to A, chromosome 14 at 118,534,144 bp
  • T to C, chromosome 15 at 8,143,161 bp
  • T to C, chromosome 15 at 44,547,103 bp
  • T to A, chromosome 15 at 89,533,213 bp
  • T to A, chromosome 17 at 12,716,650 bp
  • T to C, chromosome 17 at 17,971,079 bp
  • T to A, chromosome 17 at 30,790,833 bp
  • T to C, chromosome 17 at 38,055,329 bp
  • T to C, chromosome 18 at 31,827,406 bp
  • A to G, chromosome 18 at 43,310,477 bp
  • T to C, chromosome 18 at 84,951,364 bp
  • T to C, chromosome 19 at 8,824,986 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8994 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068825-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.