Strain Name:
C57BL/6J-MtgxR4133Btlr/Mmmh
Stock Number:
041637-MU
Citation ID:
RRID:MMRRC_041637-MU
Other Names:
R4133 (G1), C57BL/6J-MtgxR4133Btlr
Major Collection:

Strain Information

Ppp1cc
Name: protein phosphatase 1 catalytic subunit gamma
Synonyms: PP1C gamma, PP1C gamma 2, PP1, PP1C gamma 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19047
HGNC: HGNC:9283
Homologene: 135423
Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, KHS, 4432415E19Rik, GCKR
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Etv4
Name: ets variant 4
Synonyms: Pea-3, Pea3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18612
HGNC: HGNC:3493
Homologene: 1504
Smc2
Name: structural maintenance of chromosomes 2
Synonyms: CAP-E, Smc2l1, 5730502P04Rik, Fin16
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14211
Homologene: 4705
Sirt1
Name: sirtuin 1
Synonyms: Sir2, Sir2alpha
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 93759
Homologene: 56556
Washc2
Name: WASH complex subunit 2
Synonyms: Fam21, D6Wsu116e, C530005J20Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28006
Homologene: 41686
Cdc42bpb
Name: CDC42 binding protein kinase beta
Synonyms: DMPK-like
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217866
VEGA: 12
HGNC: HGNC:1738
Homologene: 55945
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978
Papola
Name: poly (A) polymerase alpha
Synonyms: Plap, PapIII
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18789
Homologene: 23389
Bop1
Name: block of proliferation 1
Synonyms: Erb1p
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12181
VEGA: 15
Homologene: 6612
Ints9
Name: integrator complex subunit 9
Synonyms: D14Ertd231e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 210925
VEGA: 14
Homologene: 10096
Mcm5
Name: minichromosome maintenance complex component 5
Synonyms: mCD46, Cdc46, Mcmd5
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17218
HGNC: HGNC:6948
Homologene: 4904
Ddx39b
Name: DEAD box helicase 39b
Synonyms: Bat1, D17H6S81E-1, Bat-1, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B, 0610030D10Rik, Bat1a
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 53817
Homologene: 48376
Cep78
Name: centrosomal protein 78
Synonyms: 5730599I05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 208518
VEGA: 19
Homologene: 11030
Trps1
Name: transcriptional repressor GATA binding 1
Synonyms: D15Ertd586e, trichorhinophalangeal syndrome I (human)
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 83925
Homologene: 8556
Arhgap42
Name: Rho GTPase activating protein 42
Synonyms: 9030420J04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71544
Homologene: 28446
Dgkd
Name: diacylglycerol kinase, delta
Synonyms: dgkd-2, DGKdelta
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227333
HGNC: HGNC:2851
Homologene: 100054
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: 8030453L17Rik, KMT2H, chromatin remodeling factor, E430018P19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192195
Homologene: 10225
Asb6
Name: ankyrin repeat and SOCS box-containing 6
Synonyms: 2510004M11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72323
Homologene: 9895
Snap91
Name: synaptosomal-associated protein 91
Synonyms: AP180, 91kDa, F1-20
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20616
Homologene: 8429
Btla
Name: B and T lymphocyte associated
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208154
Homologene: 52233
Cldn6
Name: claudin 6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 54419
HGNC: HGNC:2048
Homologene: 10300
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Khsrp
Name: KH-type splicing regulatory protein
Synonyms: KSRP, 6330409F21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16549
VEGA: 17
HGNC: HGNC:6316
Homologene: 2734
Fancm
Name: Fanconi anemia, complementation group M
Synonyms: C730036B14Rik, D12Ertd364e
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104806
VEGA: 12
Homologene: 35378
Uggt1
Name: UDP-glucose glycoprotein glucosyltransferase 1
Synonyms: A930007H10Rik, C820010P03Rik, Ugcgl1, 0910001L17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 320011
Homologene: 10586
Ssh2
Name: slingshot protein phosphatase 2
Synonyms: SSH-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237860
Homologene: 14116
Znhit3
Name: zinc finger, HIT type 3
Synonyms: Trip3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 448850
Homologene: 37964
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269700
Homologene: 28297
Gm14411
Name: predicted gene 14411
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 628239
Dennd1c
Name: DENN domain containing 1C
Synonyms: 4432409M07Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70785
VEGA: 17
Homologene: 11773
Aldh3b1
Name: aldehyde dehydrogenase 3 family, member B1
Synonyms: ALDH4, ALDH7, 1700001N19Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67689
VEGA: 19
HGNC: HGNC:410
Homologene: 73890
Mmgt2
Name: membrane magnesium transporter 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216829
Homologene: 18391
Fcer2a
Name: Fc receptor, IgE, low affinity II, alpha polypeptide
Synonyms: Ly-42, FC epsilon RII, CD23, Fce2, low-affinity IgE receptor
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14128
HGNC: HGNC:3612
Homologene: 1517
Nr3c2
Name: nuclear receptor subfamily 3, group C, member 2
Synonyms: mineralocorticoid receptor, Mlr, aldosterone receptor, MR
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 110784
HGNC: HGNC:7979
Homologene: 121495
Itga4
Name: integrin alpha 4
Synonyms: VLA-4 receptor, alpha 4 subunit
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16401
HGNC: HGNC:6140
Homologene: 37364
Ptpro
Name: protein tyrosine phosphatase receptor type O
Synonyms: D28, PTPROt, Ptpn15, PTP-oc, GLEPP1, PTP-U2, PTP-BK, PTP-phi
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19277
HGNC: HGNC:9678
Homologene: 21564
Fbn2
Name: fibrillin 2
Synonyms: Fib-2, sy, Sne
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Vmn2r3
Name: vomeronasal 2, receptor 3
Synonyms: EG637004
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 637004
Scn5a
Name: sodium channel, voltage-gated, type V, alpha
Synonyms: Nav1.5, mH1, SkM2, Nav1.5c
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20271
Homologene: 22738
Fgd5
Name: FYVE, RhoGEF and PH domain containing 5
Synonyms: ZFYVE23, C330025N11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232237
Homologene: 27798
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Nckap5
Name: NCK-associated protein 5
Synonyms: E030049G20Rik, D130011D22Rik, LOC380609
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210356
Homologene: 35542
Cpd
Name: carboxypeptidase D
Synonyms: D830034L15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12874
HGNC: HGNC:2301
Homologene: 999
Stxbp5l
Name: syntaxin binding protein 5-like
Synonyms: tomosyn-2, A830015P08Rik, insulin level locus 1, LLGL4, t2md1, T2dm1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207227
Homologene: 18173
Slco6c1
Name: solute carrier organic anion transporter family, member 6c1
Synonyms: 4933404A18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74441
Homologene: 65259
Spmap2
Name: sperm microtubule associated protein 2
Synonyms: Theg
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 21830
Homologene: 7976
Ddx60
Name: DExD/H box helicase 60
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234311
Homologene: 23031
Top2b
Name: topoisomerase (DNA) II beta
Synonyms: Top-2, D230016L12Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21974
Homologene: 134711
Arhgef4
Name: Rho guanine nucleotide exchange factor 4
Synonyms: 9330140K16Rik, C230030N03Rik, Asef
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226970
HGNC: HGNC:684
Homologene: 49414
Tex10
Name: testis expressed gene 10
Synonyms: 2810462N03Rik, clone 18330, 2610206N19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269536
Homologene: 32361
Speg
Name: SPEG complex locus
Synonyms: D1Bwg1450e, BPEG, Apeg1, SPEG, SPEGalpha, SPEGbeta
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11790
Homologene: 55619
Rpusd2
Name: RNA pseudouridylate synthase domain containing 2
Synonyms: BB231107, 4921503C21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 271842
Homologene: 5588
Garin4
Name: golgi associated RAB2 interactor family member 4
Synonyms: 4933417M04Rik, Fam71a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 619288
Homologene: 77474
Ddx31
Name: DEAD/H box helicase 31
Synonyms: 5830444G11Rik, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227674
Homologene: 6389
Or2ag16
Name: olfactory receptor family 2 subfamily AG member 16
Synonyms: GA_x6K02T2PBJ9-9130754-9129519, MOR283-3, Olfr698
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258595
Homologene: 133598
Fam234a
Name: family with sequence similarity 234, member A
Synonyms: Itfg3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106581
Homologene: 12932
Il1rap
Name: interleukin 1 receptor accessory protein
Synonyms: IL-1R AcP, 6430709H04Rik, IL-1RAcP
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16180
HGNC: HGNC:5995
Homologene: 1643
Or2t46
Name: olfactory receptor family 2 subfamily T member 46
Synonyms: MOR275-11_p, MOR275-5, Olfr325, GA_x6K02T2NKPP-844642-843680
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258261
Homologene: 133015
Rasgrf2
Name: RAS protein-specific guanine nucleotide-releasing factor 2
Synonyms: 6330417G04Rik, Grf2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19418
HGNC: HGNC:9876
Homologene: 2169
Gm6729
Name: predicted gene 6729
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 627035
VEGA: 10
Homologene: 141143
Dnah7c
Name: dynein, axonemal, heavy chain 7C
Synonyms: Dnahc7c
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100101919
Homologene: 41287
Setbp1
Name: SET binding protein 1
Synonyms: Seb
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240427
Homologene: 9192
1700036A12Rik
Name: RIKEN cDNA 1700036A12 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 100040447
Thoc3
Name: THO complex 3
Synonyms: 2410044K02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 73666
Homologene: 12505
Hspb3
Name: heat shock protein 3
Synonyms: 27kDa, 2310035K17Rik, spb3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 56534
VEGA: 13
HGNC: HGNC:5248
Homologene: 31369
Cpn1
Name: carboxypeptidase N, polypeptide 1
Synonyms: 50 kDa, CPN, 0610011F20Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 93721
HGNC: HGNC:2312
Homologene: 1002
Or9s15
Name: olfactory receptor family 9 subfamily S member 15
Synonyms: GA_x6K02T2R7CC-81157497-81156526, MOR208-3, Olfr1411
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 258483
Homologene: 115530
Arpc5
Name: actin related protein 2/3 complex, subunit 5
Synonyms: p16-Arc, 5830443F10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67771
HGNC: HGNC:708
Homologene: 4176
Igkv10-95
Name: immunoglobulin kappa variable 10-95
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 434031
Clca6
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Gm5471
Name: predicted pseudogene 5471
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 432950
VEGA: 15
Gm5478
Name: predicted pseudogene 5478
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 432987
VEGA: 15
Gm14798
Name: predicted gene 14798
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,806,104 bp
  • A to T, chromosome 1 at 36,158,159 bp
  • G to T, chromosome 1 at 46,665,990 bp
  • A to G, chromosome 1 at 75,427,904 bp
  • T to G, chromosome 1 at 87,941,501 bp
  • A to T, chromosome 1 at 88,254,965 bp
  • T to C, chromosome 1 at 92,596,743 bp
  • T to A, chromosome 1 at 97,081,493 bp
  • A to G, chromosome 1 at 126,222,706 bp
  • A to G, chromosome 1 at 152,768,871 bp
  • A to T, chromosome 1 at 191,163,008 bp
  • A to G, chromosome 2 at 28,858,852 bp
  • A to G, chromosome 2 at 30,828,235 bp
  • T to A, chromosome 2 at 79,322,652 bp
  • T to C, chromosome 2 at 119,038,715 bp
  • A to G, chromosome 2 at 177,125,697 bp
  • T to A, chromosome 3 at 64,275,717 bp
  • T to C, chromosome 3 at 88,982,260 bp
  • T to G, chromosome 3 at 144,969,352 bp
  • T to C, chromosome 4 at 48,468,968 bp
  • A to G, chromosome 4 at 52,450,947 bp
  • G to T, chromosome 5 at 121,277,834 bp
  • G to A, chromosome 5 at 122,168,226 bp
  • T to C, chromosome 6 at 68,680,617 bp
  • T to C, chromosome 6 at 92,069,437 bp
  • G to A, chromosome 6 at 116,258,930 bp
  • G to T, chromosome 6 at 137,420,372 bp
  • A to C, chromosome 7 at 106,753,079 bp
  • T to A, chromosome 8 at 3,691,130 bp
  • A to G, chromosome 8 at 61,972,220 bp
  • A to G, chromosome 8 at 75,115,854 bp
  • T to C, chromosome 8 at 76,909,749 bp
  • C to T, chromosome 9 at 9,011,299 bp
  • T to A, chromosome 9 at 60,769,998 bp
  • C to A, chromosome 9 at 86,777,049 bp
  • T to A, chromosome 9 at 119,486,372 bp
  • T to C, chromosome 10 at 63,335,659 bp
  • T to C, chromosome 10 at 79,580,050 bp
  • C to A, chromosome 10 at 86,541,166 bp
  • A to T, chromosome 11 at 58,581,075 bp
  • A to G, chromosome 11 at 62,665,051 bp
  • G to A, chromosome 11 at 76,814,818 bp
  • A to G, chromosome 11 at 77,421,269 bp
  • A to G, chromosome 11 at 84,916,313 bp
  • A to G, chromosome 11 at 94,432,544 bp
  • C to A, chromosome 11 at 101,770,498 bp
  • A to G, chromosome 12 at 65,120,530 bp
  • A to T, chromosome 12 at 69,845,723 bp
  • A to G, chromosome 12 at 105,799,658 bp
  • A to G, chromosome 12 at 111,321,542 bp
  • T to C, chromosome 13 at 54,468,548 bp
  • A to G, chromosome 13 at 91,982,654 bp
  • A to C, chromosome 13 at 113,663,491 bp
  • T to G, chromosome 14 at 16,409,189 bp
  • A to G, chromosome 14 at 64,990,554 bp
  • A to T, chromosome 15 at 44,971,901 bp
  • T to C, chromosome 15 at 50,831,387 bp
  • T to C, chromosome 15 at 76,454,335 bp
  • T to A, chromosome 15 at 101,644,645 bp
  • A to G, chromosome 16 at 26,722,886 bp
  • T to C, chromosome 16 at 37,208,119 bp
  • T to C, chromosome 16 at 45,239,298 bp
  • A to T, chromosome 17 at 23,681,493 bp
  • T to C, chromosome 17 at 26,213,558 bp
  • C to T, chromosome 17 at 35,253,089 bp
  • T to C, chromosome 17 at 57,025,605 bp
  • A to C, chromosome 17 at 57,076,980 bp
  • T to C, chromosome 17 at 67,750,655 bp
  • A to T, chromosome 17 at 67,812,486 bp
  • A to T, chromosome 18 at 58,095,962 bp
  • T to C, chromosome 18 at 78,856,991 bp
  • A to G, chromosome 19 at 3,920,808 bp
  • A to G, chromosome 19 at 15,969,155 bp
  • T to A, chromosome 19 at 36,961,738 bp
  • G to A, chromosome 19 at 43,986,284 bp
  • A to T, chromosome X at 8,369,606 bp
  • T to C, chromosome X at 94,080,858 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4133 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041637-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.