Strain Name:
C57BL/6J-MtgxR4990Btlr/Mmmh
Stock Number:
042584-MU
Citation ID:
RRID:MMRRC_042584-MU
Other Names:
R4990 (G1), C57BL/6J-MtgxR4990Btlr
Major Collection:

Strain Information

Matr3
Name: matrin 3
Synonyms: 2810017I02Rik, 1110061A14Rik, D030046F20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17184
HGNC: HGNC:6912
Homologene: 7830
Plg
Name: plasminogen
Synonyms: Pg
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18815
VEGA: 17
HGNC: HGNC:9071
Homologene: 55452
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4833417A11Rik, 4832428G11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Cadm1
Name: cell adhesion molecule 1
Synonyms: RA175A, Necl2, SgIGSF, Tslc1, RA175N, RA175C, SynCam, 3100001I08Rik, Igsf4a, Igsf4, RA175B, 2900073G06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54725
HGNC: HGNC:5951
Homologene: 8641
Kdm4c
Name: lysine (K)-specific demethylase 4C
Synonyms: 2410141F18Rik, Jmjd2c
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76804
Homologene: 41004
Emb
Name: embigin
Synonyms: Gp70
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13723
Homologene: 7743
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: b2b1625.2Clo, D230004K18Rik, Megalin, Gp330
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Nkx2-1
Name: NK2 homeobox 1
Synonyms: T/EBP, Ttf-1, Titf1, thyroid transcription factor-1, tinman, thyroid-specific enhancer-binding protein
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21869
Homologene: 2488
Triobp
Name: TRIO and F-actin binding protein
Synonyms: Tara, Mus EST 478828, EST478828
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110253
Homologene: 5104
Ipo11
Name: importin 11
Synonyms: E330021B14Rik, 1700081H05Rik, Ranbp11, 2510001A17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76582
Homologene: 7089
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243272
Homologene: 10055
Hnrnpr
Name: heterogeneous nuclear ribonucleoprotein R
Synonyms: hnRNPR, Hnrpr, 2610003J05Rik, 2610528B01Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74326
HGNC: HGNC:5047
Homologene: 4251
Wwc1
Name: WW, C2 and coiled-coil domain containing 1
Synonyms: Kibra
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211652
Homologene: 69180
Atg16l1
Name: autophagy related 16 like 1
Synonyms: APG16L, WDR30, 1500009K01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 77040
Homologene: 41786
Rasa2
Name: RAS p21 protein activator 2
Synonyms: 5430433H21Rik, GAP1m
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114713
HGNC: HGNC:9872
Homologene: 4745
Rpl4
Name: ribosomal protein L4
Synonyms: 2010004J23Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67891
VEGA: 9
Homologene: 748
Pgm3
Name: phosphoglucomutase 3
Synonyms: 2810473H05Rik, Pgm-3, GlcNAc-P mutase
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 109785
HGNC: HGNC:8907
Homologene: 9205
Panx1
Name: pannexin 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 55991
HGNC: HGNC:8599
Homologene: 49416
Ecpas
Name: Ecm29 proteasome adaptor and scaffold
Synonyms: AI314180
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230249
Homologene: 6056
Sec22b
Name: SEC22 homolog B, vesicle trafficking protein
Synonyms: Sec22l1, ERS-24, 4930564D15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20333
Homologene: 3597
Ror1
Name: receptor tyrosine kinase-like orphan receptor 1
Synonyms: Ntrkr1, 2810404D04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26563
Homologene: 3675
Irx4
Name: Iroquois homeobox 4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 50916
HGNC: HGNC:6129
Homologene: 9485
Nbeal2
Name: neurobeachin-like 2
Synonyms: 1110014F23Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235627
Homologene: 86422
Ntng1
Name: netrin G1
Synonyms: A930010C08Rik, Lmnt1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80883
Homologene: 8949
Cenpe
Name: centromere protein E
Synonyms: Kif10, 312kDa, CENP-E, N-7 kinesin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229841
HGNC: HGNC:1856
Homologene: 20429
Asph
Name: aspartate-beta-hydroxylase
Synonyms: aspartyl beta-hydroxylase, calsequestrin-binding protein, BAH, jumbug, 3110001L23Rik, junctate, cI-37, Junctin, 2310005F16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 65973
HGNC: HGNC:757
Homologene: 20910
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: 9330197E15Rik, A630028O16Rik, LOC380767
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 211978
VEGA: 12
Homologene: 9102
Svil
Name: supervillin
Synonyms: B430302E16Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225115
Homologene: 25090
Cep295
Name: centrosomal protein 295
Synonyms: LOC382128, 5830418K08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319675
Homologene: 27936
Sv2b
Name: synaptic vesicle glycoprotein 2b
Synonyms: A830038F04Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 64176
Homologene: 32236
Dock2
Name: dedicator of cyto-kinesis 2
Synonyms: CED-5, MBC, Hch
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 94176
HGNC: HGNC:2988
Homologene: 37984
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: Dbr, blanc-sale, dominant brown, Oca4, Matp, Aim-1, Aim1, bls
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 22293
Homologene: 9412
Fbxw18
Name: F-box and WD-40 domain protein 18
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 546161
Homologene: 110776
Aplnr
Name: apelin receptor
Synonyms: msr/apj, apelin receptor, APJ, Agtrl1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 23796
HGNC: HGNC:339
Homologene: 3784
Trappc11
Name: trafficking protein particle complex 11
Synonyms: D030016E14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 320714
Homologene: 11076
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20192
Homologene: 68151
D930020B18Rik
Name: RIKEN cDNA D930020B18 gene
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216393
Homologene: 87610
Dscam
Name: DS cell adhesion molecule
Synonyms: Down syndrome cell adhesion molecule, 4932410A21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13508
HGNC: HGNC:3039
Homologene: 74393
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 209012
Homologene: 41205
Tubd1
Name: tubulin, delta 1
Synonyms: 4930550G19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56427
Homologene: 9443
Cacna1b
Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms: Cchn1a, alpha(1B), Cav2.2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12287
HGNC: HGNC:1389
Homologene: 20184
Scarf1
Name: scavenger receptor class F, member 1
Synonyms: SREC, SREC-I
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380713
Homologene: 2741
Gm16432
Name: predicted gene 16432
Type: Gene
Species: Mouse
Chromosome: 1
Nt5e
Name: 5' nucleotidase, ecto
Synonyms: ecto-5'-nucleotidase, Nt5, 2210401F01Rik, CD73
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23959
HGNC: HGNC:8021
Homologene: 1895
Nrxn3
Name: neurexin III
Synonyms: neurexin III beta, neurexin III alpha, D12Bwg0831e, neurexin III alpha, 9330112C09Rik, neurexin III beta, 4933401A11Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Ms4a4a
Name: membrane-spanning 4-domains, subfamily A, member 4A
Synonyms: EG666907
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 666907
Homologene: 103874
Or13a17
Name: olfactory receptor family 13 subfamily A member 17
Synonyms: GA_x6K02T2PBJ9-42837030-42837962, MOR253-2, IB6, Olfr45
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18344
Homologene: 79416
Abca14
Name: ATP-binding cassette, sub-family A member 14
Synonyms: 1700110B15Rik, 4930539G24Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67928
Homologene: 86128
Megf6
Name: multiple EGF-like-domains 6
Synonyms: Egfl3, 2600001P17Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230971
HGNC: HGNC:3232
Homologene: 45412
Kcnh7
Name: potassium voltage-gated channel, subfamily H (eag-related), member 7
Synonyms: Kv11.3, erg3, 9330137I11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 170738
Homologene: 13249
Amer3
Name: APC membrane recruitment 3
Synonyms: Fam123c, 9430069J07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 211383
Homologene: 51888
Tmprss5
Name: transmembrane protease, serine 5 (spinesin)
Synonyms: spinesin
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 80893
Homologene: 69471
Pon3
Name: paraoxonase 3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 269823
HGNC: HGNC:9206
Homologene: 37371
Eno3
Name: enolase 3, beta muscle
Synonyms: Eno-3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13808
HGNC: HGNC:3354
Homologene: 68215
Or9g20
Name: olfactory receptor family 9 subfamily G member 20
Synonyms: Olfr1016, GA_x6K02T2Q125-47278889-47277960, MOR213-9
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 257915
Homologene: 83133
Elmo1
Name: engulfment and cell motility 1
Synonyms: C230095H21Rik, 6330578D22Rik, CED-12
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 140580
Homologene: 56685
Or3a1d
Name: olfactory receptor family 3 subfamily A member 1D
Synonyms: MOR255-3, GA_x6K02T2P1NL-4481525-4480578, Olfr411
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258704
Homologene: 74999
Klk1b26
Name: kallikrein 1-related petidase b26
Synonyms: Klk26, Egfbp2, PRECE-2, mGK-26, EGF-BP type B
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16618
Homologene: 68141
Fbp1
Name: fructose bisphosphatase 1
Synonyms: FBPase liver, FBPase brain isoform, Fbp-2, Fbp3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14121
HGNC: HGNC:3606
Homologene: 55467
Pcsk4
Name: proprotein convertase subtilisin/kexin type 4
Synonyms: PC4, SPC5
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18551
HGNC: HGNC:8746
Homologene: 22495
Ccdc152
Name: coiled-coil domain containing 152
Synonyms: ENSMUSG00000071814
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 100039139
VEGA: 15
Homologene: 79247
Mesp1
Name: mesoderm posterior 1
Synonyms: bHLHc5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17292
Homologene: 32039
Snai3
Name: snail family zinc finger 3
Synonyms: Zfp293, Smuc, Snail-related gene from muscle cells
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 30927
Homologene: 8500
Speer1e
Name: spermatogenesis associated glutamate (E)-rich protein 1E
Synonyms: Gm5861
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 545728
Vmn2r39
Name: vomeronasal 2, receptor 39
Synonyms: EG545909
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 545909
Homologene: 113703
Zfp131
Name: zinc finger protein 131
Synonyms: Znf131, 2610109I01Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72465
VEGA: 13
Homologene: 12464
Or4c110
Name: olfactory receptor family 4 subfamily C member 110
Synonyms: MOR233-13, GA_x6K02T2Q125-50482823-50481885, Olfr1215
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258451
Homologene: 74071
Spata31d1a
Name: spermatogenesis associated 31 subfamily D, member 1A
Synonyms: 1700013B16Rik, Fam75d3, Fam75d1a
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72219
VEGA: 13
Homologene: 122131
Grem2
Name: gremlin 2, DAN family BMP antagonist
Synonyms: Prdc
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23893
Homologene: 8023
Gm11100
Name: predicted gene 11100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 102633458
Homologene: 131552
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 34,588,741 bp
  • T to C, chromosome 1 at 71,294,939 bp
  • A to G, chromosome 1 at 87,789,369 bp
  • A to G, chromosome 1 at 174,836,813 bp
  • A to C, chromosome 1 at 178,098,421 bp
  • A to G, chromosome 2 at 24,678,874 bp
  • C to T, chromosome 2 at 52,255,546 bp
  • T to C, chromosome 2 at 62,734,288 bp
  • G to T, chromosome 2 at 69,481,388 bp
  • G to A, chromosome 2 at 85,137,377 bp
  • A to T, chromosome 2 at 85,800,297 bp
  • A to T, chromosome 2 at 89,001,472 bp
  • G to T, chromosome 2 at 112,635,777 bp
  • T to C, chromosome 2 at 112,909,973 bp
  • T to G, chromosome 3 at 97,921,111 bp
  • A to T, chromosome 3 at 110,135,261 bp
  • T to A, chromosome 3 at 135,256,640 bp
  • G to T, chromosome 4 at 9,643,554 bp
  • A to G, chromosome 4 at 58,822,104 bp
  • A to G, chromosome 4 at 74,262,936 bp
  • A to G, chromosome 4 at 100,441,964 bp
  • A to G, chromosome 4 at 136,329,379 bp
  • G to A, chromosome 4 at 136,336,298 bp
  • A to G, chromosome 4 at 154,267,226 bp
  • A to T, chromosome 5 at 11,186,414 bp
  • A to T, chromosome 5 at 124,400,165 bp
  • T to C, chromosome 6 at 5,221,619 bp
  • A to G, chromosome 7 at 9,023,676 bp
  • T to A, chromosome 7 at 44,016,249 bp
  • T to C, chromosome 7 at 75,117,722 bp
  • A to G, chromosome 7 at 79,792,921 bp
  • G to A, chromosome 7 at 120,312,165 bp
  • G to A, chromosome 7 at 140,691,447 bp
  • A to T, chromosome 8 at 47,490,895 bp
  • G to A, chromosome 8 at 122,456,332 bp
  • A to G, chromosome 9 at 15,010,217 bp
  • T to C, chromosome 9 at 15,332,138 bp
  • T to A, chromosome 9 at 47,789,689 bp
  • A to G, chromosome 9 at 49,102,815 bp
  • T to C, chromosome 9 at 64,174,885 bp
  • T to A, chromosome 9 at 86,558,412 bp
  • A to G, chromosome 9 at 88,355,593 bp
  • A to G, chromosome 9 at 96,591,989 bp
  • T to C, chromosome 9 at 109,688,393 bp
  • A to C, chromosome 9 at 110,634,803 bp
  • C to T, chromosome 9 at 121,192,786 bp
  • A to G, chromosome 10 at 80,325,381 bp
  • C to G, chromosome 10 at 121,654,761 bp
  • C to T, chromosome 10 at 121,654,762 bp
  • T to C, chromosome 11 at 34,695,251 bp
  • T to C, chromosome 11 at 35,876,566 bp
  • A to G, chromosome 11 at 70,658,647 bp
  • A to G, chromosome 11 at 74,347,197 bp
  • T to C, chromosome 11 at 75,526,015 bp
  • T to C, chromosome 11 at 86,557,839 bp
  • T to C, chromosome 12 at 56,534,939 bp
  • T to C, chromosome 12 at 79,287,833 bp
  • T to A, chromosome 12 at 89,260,474 bp
  • T to C, chromosome 13 at 20,342,519 bp
  • T to C, chromosome 13 at 59,703,151 bp
  • C to T, chromosome 13 at 62,865,074 bp
  • G to T, chromosome 13 at 73,265,507 bp
  • A to T, chromosome 13 at 106,860,887 bp
  • T to A, chromosome 13 at 117,264,510 bp
  • A to C, chromosome 13 at 119,782,913 bp
  • A to C, chromosome 14 at 13,949,220 bp
  • A to G, chromosome 15 at 3,301,157 bp
  • A to G, chromosome 15 at 11,001,150 bp
  • C to A, chromosome 15 at 78,967,005 bp
  • A to G, chromosome 16 at 96,825,515 bp
  • T to C, chromosome 17 at 12,411,510 bp
  • T to A, chromosome 18 at 5,056,810 bp
  • T to A, chromosome 18 at 35,572,963 bp
  • T to C, chromosome 19 at 11,378,837 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4990 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042584-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.