Strain Name:
C57BL/6J-MtgxR5177Btlr/Mmmh
Stock Number:
042757-MU
Citation ID:
RRID:MMRRC_042757-MU
Other Names:
R5177 (G1), C57BL/6J-MtgxR5177Btlr
Major Collection:

Strain Information

Rc3h1
Name: RING CCCH (C3H) domains 1
Synonyms: roquin, 5730557L09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381305
Homologene: 19036
Lias
Name: lipoic acid synthetase
Synonyms: MGC7254, mLip1, 4933425M12Rik, 7a5ex, 2900022L22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 79464
Homologene: 4997
Mrps18b
Name: mitochondrial ribosomal protein S18B
Synonyms: 2400002C15Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66973
Homologene: 32205
Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: Edd1, Edd, 4432411E13Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Cltc
Name: clathrin heavy chain
Synonyms: CHC
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67300
HGNC: HGNC:2092
Homologene: 3572
Map4k4
Name: mitogen-activated protein kinase kinase kinase kinase 4
Synonyms: Nik, 9430080K19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26921
HGNC: HGNC:6866
Homologene: 7442
Klhdc4
Name: kelch domain containing 4
Synonyms: G430025P05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234825
Homologene: 69234
Dennd2c
Name: DENN domain containing 2C
Synonyms: A930010I20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329727
Homologene: 19542
Nme7
Name: NME/NM23 family member 7
Synonyms: non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase), nucleoside-diphosphate kinase, Nm23-M7, D530024H21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 171567
Homologene: 8344
Myo18a
Name: myosin XVIIIA
Synonyms: MyoPDZ
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 360013
Homologene: 7977
Bltp3a
Name: bridge-like lipid transfer protein family member 3A
Synonyms: 1110020K19Rik, Uhrf1bp1, F830021D11Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224648
Homologene: 9816
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Prpf3
Name: pre-mRNA processing factor 3
Synonyms: 3632413F13Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70767
Homologene: 3447
Rasa2
Name: RAS p21 protein activator 2
Synonyms: 5430433H21Rik, GAP1m
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114713
HGNC: HGNC:9872
Homologene: 4745
Tacc1
Name: transforming, acidic coiled-coil containing protein 1
Synonyms: 4833447E04Rik, Tacc1, B230378H13Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 320165
Homologene: 4575
Nbn
Name: nibrin
Synonyms: Nbs1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 27354
HGNC: HGNC:7652
Homologene: 1858
Socs6
Name: suppressor of cytokine signaling 6
Synonyms: HSPC060, CIS4, SSI4, STATI4, SOCS-4, 5830401B18Rik, 1500012M23Rik, SOCS-6, STAI4, Cish4, STAT4, Socs4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 54607
Homologene: 3120
Rhot1
Name: ras homolog family member T1
Synonyms: Arht1, Miro1, 2210403N23Rik, FLJ11040, C430039G08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 59040
Homologene: 56803
Ern1
Name: endoplasmic reticulum to nucleus signalling 1
Synonyms: Ire1alpha, 9030414B18Rik, Ire1p, Ire1a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78943
HGNC: HGNC:3449
Homologene: 55580
Epgn
Name: epithelial mitogen
Synonyms: 2310069M11Rik, epigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71920
Homologene: 19527
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: Plc, perlecan, Pcn, per
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Dcbld1
Name: discoidin, CUB and LCCL domain containing 1
Synonyms: 4631413K11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66686
Homologene: 12010
Nol8
Name: nucleolar protein 8
Synonyms: 4921532D18Rik, 5730412B09Rik, D13Ertd548e
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 70930
VEGA: 13
Homologene: 41210
Ppp1r12c
Name: protein phosphatase 1, regulatory subunit 12C
Synonyms: Mbs85, 2410197A17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232807
Homologene: 75071
Tmprss4
Name: transmembrane protease, serine 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214523
Homologene: 80930
Dnajc13
Name: DnaJ heat shock protein family (Hsp40) member C13
Synonyms: LOC382100, D030002L11Rik, Rme8
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235567
Homologene: 13574
Asic4
Name: acid-sensing ion channel family member 4
Synonyms: Accn4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241118
Homologene: 11166
Atp2b4
Name: ATPase, Ca++ transporting, plasma membrane 4
Synonyms: PMCA4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381290
HGNC: HGNC:817
Homologene: 48034
Lama2
Name: laminin, alpha 2
Synonyms: merosin, mer
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Rusc2
Name: RUN and SH3 domain containing 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100213
Homologene: 18967
Vmn2r57
Name: vomeronasal 2, receptor 57
Synonyms: EG269902
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269902
Homologene: 104832
Trpm1
Name: transient receptor potential cation channel, subfamily M, member 1
Synonyms: 4732499L03Rik, Mlsn1, melastatin, LTRPC1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17364
HGNC: HGNC:7146
Homologene: 19940
Rabl6
Name: RAB, member RAS oncogene family-like 6
Synonyms: B230208H17Rik, Rbel1a, Rbel1b, Rbel1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227624
Homologene: 11674
Abcb11
Name: ATP-binding cassette, sub-family B member 11
Synonyms: ABC16, Lith1, PFIC2, sister of P-glycoprotein, Bsep, PGY4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27413
HGNC: HGNC:42
Homologene: 74509
Uba5
Name: ubiquitin-like modifier activating enzyme 5
Synonyms: Ube1dc1, 5730525G14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66663
Homologene: 11738
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: E230008N13Rik, LOC381522
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381522
Homologene: 117988
F12
Name: coagulation factor XII (Hageman factor)
Synonyms: FXII
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 58992
HGNC: HGNC:3530
Homologene: 425
Trip6
Name: thyroid hormone receptor interactor 6
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22051
Homologene: 37757
Dpy19l1
Name: dpy-19 like C-mannosyltransferase 1
Synonyms: 1100001I19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244745
Homologene: 14762
Thsd7a
Name: thrombospondin, type I, domain containing 7A
Synonyms: LOC330267
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330267
Homologene: 46582
Vmn2r79
Name: vomeronasal 2, receptor 79
Synonyms: EG621430
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 621430
Homologene: 115466
Gal3st2c
Name: galactose-3-O-sulfotransferase 2C
Synonyms: Gm6086
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 619597
Homologene: 41471
Matn2
Name: matrilin 2
Synonyms: Crtm2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17181
VEGA: 15
HGNC: HGNC:6908
Homologene: 20538
Nek8
Name: NIMA (never in mitosis gene a)-related expressed kinase 8
Synonyms: 4632401F23Rik, b2b1449Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 140859
Homologene: 84442
Pla2g4b
Name: phospholipase A2, group IVB (cytosolic)
Synonyms: A030011C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 211429
Homologene: 3730
Slc34a1
Name: solute carrier family 34 (sodium phosphate), member 1
Synonyms: Na/Pi cotransporter, Slc17a2, Npt2, NaPi-IIa, renal Na+/Pi transporter
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20505
VEGA: 13
Homologene: 20663
Myorg
Name: myogenesis regulating glycosidase (putative)
Synonyms: NET37, AI464131
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329828
Homologene: 19853
Cylc2
Name: cylicin, basic protein of sperm head cytoskeleton 2
Synonyms: 4930488P18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74914
HGNC: HGNC:2583
Homologene: 136270
Rimbp3
Name: RIMS binding protein 3
Synonyms: LOC385766, LOC239731, RIM-BP3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239731
Homologene: 77940
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 620697
Homologene: 115466
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Oprk1
Name: opioid receptor, kappa 1
Synonyms: KOR-1, Oprk2, R21
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18387
HGNC: HGNC:8154
Homologene: 20253
Nostrin
Name: nitric oxide synthase trafficker
Synonyms: mDaIP2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329416
Homologene: 43142
Hmgxb3
Name: HMG box domain containing 3
Synonyms: 2510002C16Rik, A630042L21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 106894
VEGA: 18
Homologene: 44229
Polrmt
Name: polymerase (RNA) mitochondrial (DNA directed)
Synonyms: 1110018N15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216151
HGNC: HGNC:9200
Homologene: 37996
Arhgap22
Name: Rho GTPase activating protein 22
Synonyms: B230341L19Rik, RHOGAP2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239027
Homologene: 23292
Galnt7
Name: polypeptide N-acetylgalactosaminyltransferase 7
Synonyms: ppGaNTase-T7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 108150
HGNC: HGNC:4129
Homologene: 9685
Kif12
Name: kinesin family member 12
Synonyms: N-9 kinesin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16552
Homologene: 7796
Cfb
Name: complement factor B
Synonyms: Factor B, H2-Bf, alternative-complement pathway C3/C5 convertase, Bf, B, FB
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14962
HGNC: HGNC:1037
Homologene: 1292
Vps16
Name: VSP16 CORVET/HOPS core subunit
Synonyms: 1810074M16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 80743
Homologene: 7116
Slc25a11
Name: solute carrier family 25 (mitochondrial carrier oxoglutarate carrier), member 11
Synonyms: 2oxoc, 2310022P18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67863
Homologene: 2637
Or13a18
Name: olfactory receptor family 13 subfamily A member 18
Synonyms: MOR253-8, GA_x6K02T2PBJ9-42759973-42760905, IF5, ID12, IB7, Olfr46
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18345
Homologene: 110491
Srl
Name: sarcalumenin
Synonyms: 9830004M20Rik, sar, sarcalumenin
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 106393
Homologene: 45500
Gimap1
Name: GTPase, IMAP family member 1
Synonyms: Imap38, IAP38, 38kDa, imap
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16205
Homologene: 7379
Ears2
Name: glutamyl-tRNA synthetase 2, mitochondrial
Synonyms: 3230401I01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67417
Homologene: 6907
Hddc3
Name: HD domain containing 3
Synonyms: 1110033O09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68695
Homologene: 12279
Tlr12
Name: toll-like receptor 12
Synonyms: Tlr11, LOC384059
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 384059
Homologene: 135964
Zfp783
Name: zinc finger protein 783
Synonyms: Gm20494, ENSMUSG00000072653, A230106D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232785
Or52j3
Name: olfactory receptor family 52 subfamily J member 3
Synonyms: MOR0-3P, GA_x6K02T2PBJ9-5902266-5903204, MOR0-3P, Olfr592, Olfr1525-ps1, MOR32-13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 404317
Homologene: 66215
Gm5414
Name: predicted gene 5414
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 406223
VEGA: 15
Sox12
Name: SRY (sex determining region Y)-box 12
Synonyms: 2010205A02Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20667
Homologene: 5057
Cfap91
Name: cilia and flagella associated protein 91
Synonyms: Spata26, 4932425I24Rik, Maats1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 320214
Homologene: 33470
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 5,602,674 bp
  • A to G, chromosome 1 at 39,986,762 bp
  • T to C, chromosome 1 at 75,450,839 bp
  • C to T, chromosome 1 at 94,009,208 bp
  • T to C, chromosome 1 at 133,728,768 bp
  • G to T, chromosome 1 at 160,951,652 bp
  • T to G, chromosome 1 at 164,380,676 bp
  • T to C, chromosome 2 at 25,585,373 bp
  • A to T, chromosome 2 at 69,175,754 bp
  • C to T, chromosome 2 at 69,285,295 bp
  • T to A, chromosome 2 at 120,041,601 bp
  • G to T, chromosome 2 at 130,443,368 bp
  • A to T, chromosome 2 at 152,397,178 bp
  • T to A, chromosome 3 at 95,849,724 bp
  • G to T, chromosome 3 at 103,157,812 bp
  • A to T, chromosome 4 at 15,965,132 bp
  • C to A, chromosome 4 at 41,498,407 bp
  • A to T, chromosome 4 at 43,421,805 bp
  • A to T, chromosome 4 at 45,917,508 bp
  • T to C, chromosome 4 at 51,228,587 bp
  • G to A, chromosome 4 at 63,167,904 bp
  • A to C, chromosome 4 at 128,618,376 bp
  • A to C, chromosome 4 at 137,518,772 bp
  • G to A, chromosome 5 at 65,392,237 bp
  • A to C, chromosome 5 at 91,028,277 bp
  • T to C, chromosome 5 at 137,312,172 bp
  • A to T, chromosome 6 at 12,379,583 bp
  • A to G, chromosome 6 at 47,946,803 bp
  • G to T, chromosome 6 at 48,743,098 bp
  • T to A, chromosome 7 at 4,484,496 bp
  • A to G, chromosome 7 at 41,400,240 bp
  • C to T, chromosome 7 at 64,203,016 bp
  • A to G, chromosome 7 at 80,343,166 bp
  • T to A, chromosome 7 at 85,221,991 bp
  • T to A, chromosome 7 at 87,001,969 bp
  • A to T, chromosome 7 at 103,187,503 bp
  • A to G, chromosome 7 at 122,044,460 bp
  • A to G, chromosome 7 at 140,610,189 bp
  • A to T, chromosome 8 at 25,201,221 bp
  • C to A, chromosome 8 at 57,584,027 bp
  • A to T, chromosome 8 at 121,813,790 bp
  • C to T, chromosome 9 at 24,438,628 bp
  • C to A, chromosome 9 at 45,173,962 bp
  • C to A, chromosome 9 at 96,544,791 bp
  • T to G, chromosome 9 at 104,049,298 bp
  • G to A, chromosome 9 at 104,230,986 bp
  • C to T, chromosome 10 at 27,190,703 bp
  • T to A, chromosome 10 at 52,304,634 bp
  • T to C, chromosome 10 at 79,737,476 bp
  • C to T, chromosome 11 at 60,712,007 bp
  • T to C, chromosome 11 at 70,645,817 bp
  • A to G, chromosome 11 at 77,864,842 bp
  • G to A, chromosome 11 at 78,170,471 bp
  • A to T, chromosome 11 at 80,246,766 bp
  • T to C, chromosome 11 at 86,705,163 bp
  • T to C, chromosome 11 at 106,411,775 bp
  • A to T, chromosome 13 at 49,661,112 bp
  • A to T, chromosome 13 at 55,401,162 bp
  • G to A, chromosome 13 at 55,420,168 bp
  • T to G, chromosome 14 at 33,366,693 bp
  • A to G, chromosome 15 at 34,433,514 bp
  • A to G, chromosome 15 at 38,006,517 bp
  • A to G, chromosome 15 at 101,625,817 bp
  • A to G, chromosome 16 at 4,496,403 bp
  • G to A, chromosome 16 at 17,209,917 bp
  • A to G, chromosome 16 at 38,332,321 bp
  • T to C, chromosome 17 at 27,885,018 bp
  • A to G, chromosome 17 at 34,859,026 bp
  • A to T, chromosome 17 at 35,914,525 bp
  • T to C, chromosome 18 at 49,893,584 bp
  • T to C, chromosome 18 at 61,172,194 bp
  • A to C, chromosome 18 at 88,869,380 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5177 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042757-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.