Strain Name:
C57BL/6J-MtgxR5212Btlr/Mmmh
Stock Number:
042786-MU
Citation ID:
RRID:MMRRC_042786-MU
Other Names:
R5212 (G1), C57BL/6J-MtgxR5212Btlr
Major Collection:

Strain Information

Arhgef7
Name: Rho guanine nucleotide exchange factor
Synonyms: PIX, betaPix-b, betaPix, Pak interacting exchange factor, p85SPR, betaPix-c, Cool, cool-1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54126
Homologene: 2895
Dock1
Name: dedicator of cytokinesis 1
Synonyms: Dock180, b2b3190Clo, 9130006G06Rik, D630004B07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Cdk11b
Name: cyclin dependent kinase 11B
Synonyms: Cdc2l1, CDK11-p58, CDK11-p46, PITSLRE proteins, CDK11-p110, Cdc2l2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12537
Homologene: 22416
Madcam1
Name: mucosal vascular addressin cell adhesion molecule 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17123
VEGA: 10
HGNC: HGNC:6765
Homologene: 8413
Ggnbp2
Name: gametogenetin binding protein 2
Synonyms: Zfp403, D330017P12Rik, DIF-3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217039
Homologene: 11750
Lig3
Name: ligase III, DNA, ATP-dependent
Synonyms: D11Wsu78e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16882
HGNC: HGNC:6600
Homologene: 32109
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: D630005A10Rik, apollon, A430032G04Rik, A430040A19Rik, Bruce
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12211
Homologene: 7248
Kifbp
Name: kinesin family binding protein
Synonyms: 2510003E04Rik, Kif1bp
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72320
Homologene: 9223
Wls
Name: wntless WNT ligand secretion mediator
Synonyms: Gpr177, 5031439A09Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68151
Homologene: 11779
Fnta
Name: farnesyltransferase, CAAX box, alpha
Synonyms: FTA
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14272
HGNC: HGNC:3782
Homologene: 1534
Emc2
Name: ER membrane protein complex subunit 2
Synonyms: 4921531G14Rik, Ttc35
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66736
VEGA: 15
Homologene: 8785
Tdrd3
Name: tudor domain containing 3
Synonyms: 4732418C03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219249
Homologene: 12771
Cep350
Name: centrosomal protein 350
Synonyms: 4933409L06Rik, 6430546F08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74081
Homologene: 8879
Atg2b
Name: autophagy related 2B
Synonyms: C030004M05Rik, 2410024A21Rik, C630028L02Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 76559
VEGA: 12
Homologene: 9974
Tmprss6
Name: transmembrane serine protease 6
Synonyms: matriptase-2, 1300008A22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 71753
VEGA: 15
Homologene: 12408
Ncbp3
Name: nuclear cap binding subunit 3
Synonyms: 1200014J11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66874
Homologene: 10231
Nufip1
Name: nuclear FMR1 interacting protein 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 27275
VEGA: 14
HGNC: HGNC:8057
Homologene: 8216
Arglu1
Name: arginine and glutamate rich 1
Synonyms: 9430010O03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234023
Homologene: 9960
Ccp110
Name: centriolar coiled coil protein 110
Synonyms: 6330503K22Rik, CP110
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101565
Homologene: 8810
Rsbn1l
Name: round spermatid basic protein 1-like
Synonyms: C330002G24Rik, 8430412F05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 242860
Homologene: 19435
Gas2l1
Name: growth arrest-specific 2 like 1
Synonyms: TU-71.1, GAR22, 4930500E24Rik, D0Jmb1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 78926
Homologene: 4733
Cd63
Name: CD63 antigen
Synonyms: Tspan30, ME491
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12512
VEGA: 10
HGNC: HGNC:1692
Homologene: 37526
Mindy1
Name: MINDY lysine 48 deubiquitinase 1
Synonyms: 4930504E06Rik, NF-E2 inducible protein, Fam63a, 1810005H09Rik, cI-40
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 75007
Homologene: 32409
Tmprss2
Name: transmembrane protease, serine 2
Synonyms: D16Ertd61e, epitheliasin
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 50528
VEGA: 16
Homologene: 4136
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: Dbr, blanc-sale, dominant brown, Oca4, Matp, Aim-1, Aim1, bls
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 22293
Homologene: 9412
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Itga4
Name: integrin alpha 4
Synonyms: VLA-4 receptor, alpha 4 subunit
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16401
HGNC: HGNC:6140
Homologene: 37364
Ush2a
Name: usherin
Synonyms: MUSH2A, LOC269160, Ush2a, A930037M10Rik, A930011D15Rik, LOC381317, Ushrn
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 22283
Homologene: 66151
Spaca6
Name: sperm acrosome associated 6
Synonyms: Ncrna00085, 4930546H06Rik, B230206P06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75202
Homologene: 53443
1110002E22Rik
Name: RIKEN cDNA 1110002E22 gene
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 102634333
Homologene: 131709
Jakmip1
Name: janus kinase and microtubule interacting protein 1
Synonyms: Gababrbp, Marlin-1, 5830437M04Rik, C330021K24Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76071
Homologene: 90789
Abca9
Name: ATP-binding cassette, sub-family A member 9
Synonyms: D630040K07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217262
HGNC: HGNC:39
Homologene: 33332
Fshr
Name: follicle stimulating hormone receptor
Synonyms: FSH-R, Follitropin receptor, follicle-stimulating hormone receptor
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14309
VEGA: 17
HGNC: HGNC:3969
Homologene: 117
Crybg1
Name: crystallin beta-gamma domain containing 1
Synonyms: Aim1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11630
HGNC: HGNC:356
Homologene: 18168
Rnpepl1
Name: arginyl aminopeptidase (aminopeptidase B)-like 1
Synonyms: 1110014H17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108657
Homologene: 36367
Adamtsl4
Name: ADAMTS-like 4
Synonyms: Tsrc1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229595
Homologene: 23141
Snap47
Name: synaptosomal-associated protein, 47
Synonyms: 1110031B06Rik, SNAP-47
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67826
Homologene: 14206
Nek8
Name: NIMA (never in mitosis gene a)-related expressed kinase 8
Synonyms: 4632401F23Rik, b2b1449Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 140859
Homologene: 84442
F13b
Name: coagulation factor XIII, beta subunit
Synonyms: Cf-13b, Cf13b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14060
HGNC: HGNC:3534
Homologene: 1512
Clip1
Name: CAP-GLY domain containing linker protein 1
Synonyms: Clip 170, Clip50, CLIP-170, 4631429H07Rik, cytoplasmic linker protein 50, Rsn, restin, 1110007I12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56430
Homologene: 74455
Krt82
Name: keratin 82
Synonyms: Krt2-20
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 114566
VEGA: 15
HGNC: HGNC:6459
Homologene: 13200
Krt8
Name: keratin 8
Synonyms: EndoA, cytokeratin8, cytokeratin-8, K8, Krt2-8, Card2, cytokeratin 8, Krt-2.8
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16691
VEGA: 15
HGNC: HGNC:6446
Homologene: 55643
Pbx3
Name: pre B cell leukemia homeobox 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18516
HGNC: HGNC:8634
Homologene: 21243
Amer2
Name: APC membrane recruitment 2
Synonyms: 2600011E07Rik, Fam123a, Amer2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 72125
VEGA: 14
Homologene: 45141
Setd7
Name: SET domain containing (lysine methyltransferase) 7
Synonyms: Set7/9, 1600028F23Rik, Set7, KMT7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 73251
Homologene: 12741
Mtss2
Name: MTSS I-BAR domain containing 2
Synonyms: Mtss1l, ABBA
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244654
Homologene: 70926
Gm7732
Name: predicted gene 7732
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 665654
Hand1
Name: heart and neural crest derivatives expressed 1
Synonyms: Ehand1, bHLHa27, Thing1, Th1, Hxt, eHAND
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15110
HGNC: HGNC:4807
Homologene: 3545
Plppr3
Name: phospholipid phosphatase related 3
Synonyms: Lppr3, BC005764
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216152
Homologene: 11769
Nans
Name: N-acetylneuraminic acid synthase (sialic acid synthase)
Synonyms: N-acetylneuraminic acid phosphate synthase, 4632418E04Rik, Sas
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 94181
Homologene: 10343
Trav6-1
Name: T cell receptor alpha variable 6-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 667449
C330011M18Rik
Name: RIKEN cDNA C330011M18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Gm6124
Name: predicted gene 6124
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620018
Homologene: 45597
Gm20517
Name: predicted gene 20517
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 112694759
Gm7489
Name: predicted gene 7489
Type: Gene
Species: Mouse
Chromosome: 15
Bhlhe23
Name: basic helix-loop-helix family, member e23
Synonyms: beta-cell E-box transactivating factor 4, Bhlhb4, A930001L02Rik, BETA4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 140489
Homologene: 50956
Gm3371
Name: predicted gene 3371
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100861814
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 92,911,323 bp
  • A to T, chromosome 1 at 139,512,987 bp
  • T to C, chromosome 1 at 155,875,437 bp
  • T to A, chromosome 1 at 188,444,705 bp
  • T to C, chromosome 2 at 34,288,781 bp
  • T to C, chromosome 2 at 76,789,601 bp
  • T to A, chromosome 2 at 76,797,675 bp
  • C to T, chromosome 2 at 76,901,613 bp
  • A to C, chromosome 2 at 79,280,595 bp
  • T to A, chromosome 2 at 180,776,093 bp
  • A to T, chromosome 3 at 51,542,817 bp
  • G to T, chromosome 3 at 95,294,803 bp
  • T to A, chromosome 3 at 95,677,670 bp
  • T to A, chromosome 3 at 138,065,850 bp
  • A to G, chromosome 3 at 159,873,008 bp
  • T to C, chromosome 4 at 46,502,547 bp
  • T to C, chromosome 4 at 155,638,615 bp
  • A to T, chromosome 5 at 20,896,214 bp
  • A to T, chromosome 5 at 37,104,901 bp
  • T to A, chromosome 5 at 123,630,681 bp
  • A to G, chromosome 7 at 39,223,004 bp
  • C to T, chromosome 7 at 118,729,696 bp
  • A to C, chromosome 7 at 134,789,194 bp
  • T to A, chromosome 8 at 8,683,843 bp
  • A to G, chromosome 8 at 11,728,388 bp
  • A to G, chromosome 8 at 26,009,707 bp
  • G to T, chromosome 8 at 84,066,592 bp
  • A to G, chromosome 8 at 110,729,218 bp
  • A to T, chromosome 10 at 43,967,743 bp
  • A to T, chromosome 10 at 62,563,129 bp
  • C to G, chromosome 10 at 79,668,345 bp
  • C to T, chromosome 10 at 79,862,445 bp
  • A to G, chromosome 10 at 128,911,853 bp
  • A to T, chromosome 11 at 5,061,108 bp
  • A to C, chromosome 11 at 57,831,447 bp
  • T to C, chromosome 11 at 59,428,352 bp
  • A to G, chromosome 11 at 73,053,547 bp
  • T to C, chromosome 11 at 78,172,516 bp
  • A to G, chromosome 11 at 82,787,678 bp
  • T to A, chromosome 11 at 84,854,021 bp
  • T to C, chromosome 11 at 110,107,226 bp
  • A to G, chromosome 12 at 105,646,796 bp
  • A to C, chromosome 14 at 44,403,654 bp
  • A to T, chromosome 14 at 52,638,704 bp
  • A to G, chromosome 14 at 60,379,820 bp
  • A to T, chromosome 14 at 76,133,098 bp
  • G to C, chromosome 14 at 87,506,215 bp
  • C to T, chromosome 15 at 11,027,785 bp
  • A to G, chromosome 15 at 43,510,844 bp
  • T to A, chromosome 15 at 53,885,620 bp
  • A to G, chromosome 15 at 78,446,260 bp
  • T to C, chromosome 15 at 101,545,049 bp
  • G to T, chromosome 15 at 101,997,967 bp
  • G to T, chromosome 16 at 97,576,292 bp
  • C to A, chromosome 17 at 17,838,394 bp
  • T to C, chromosome 17 at 21,130,084 bp
  • T to C, chromosome 17 at 47,618,850 bp
  • A to C, chromosome 17 at 74,670,374 bp
  • T to A, chromosome 17 at 88,986,256 bp
  • T to A, chromosome 17 at 88,986,257 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5212 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042786-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.