Strain Name:
C57BL/6J-MtgxR5224Btlr/Mmmh
Stock Number:
042797-MU
Citation ID:
RRID:MMRRC_042797-MU
Other Names:
R5224 (G1), C57BL/6J-MtgxR5224Btlr
Major Collection:

Strain Information

Mga
Name: MAX gene associated
Synonyms: Mga, D030062C11Rik, C130042M01Rik, Mad5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: G430090F17Rik, LOC328902
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Abtb3
Name: ankyrin repeat and BTB domain containing 3
Synonyms: Btbd11, 6330404E16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74007
Homologene: 72536
Ldlrad3
Name: low density lipoprotein receptor class A domain containing 3
Synonyms: Lrad3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241576
Homologene: 18377
Camk1g
Name: calcium/calmodulin-dependent protein kinase I gamma
Synonyms: CaMKIgamma, CLICK-III
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215303
Homologene: 48706
Washc2
Name: WASH complex subunit 2
Synonyms: Fam21, D6Wsu116e, C530005J20Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28006
Homologene: 41686
Erp44
Name: endoplasmic reticulum protein 44
Synonyms: Txndc4, 1110001E24Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76299
Homologene: 12638
Suco
Name: SUN domain containing ossification factor
Synonyms: osteopotentia, AI848100, Opt
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226551
HGNC: HGNC:1240
Homologene: 32212
Gphn
Name: gephyrin
Synonyms: geph, 5730552E08Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 268566
VEGA: 12
Homologene: 10820
Hnrnpul1
Name: heterogeneous nuclear ribonucleoprotein U-like 1
Synonyms: E1B-AP5, E1BAP5, E130317O14Rik, Hnrnpul, Hnrpul1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232989
Homologene: 31419
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: Mypt1, 5730577I22Rik, D10Ertd625e, 1200015F06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Nav2
Name: neuron navigator 2
Synonyms: Rainb1, POMFIL2, Unc53H2, 5330421F07Rik, HELAD1, RAINB2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78286
Homologene: 52330
Depdc1b
Name: DEP domain containing 1B
Synonyms: XTP1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218581
Homologene: 10157
Cct3
Name: chaperonin containing TCP1 subunit 3
Synonyms: TriC-P5, Cctg, Tcp1-rs3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12462
HGNC: HGNC:1616
Homologene: 4373
Tram1
Name: translocating chain-associating membrane protein 1
Synonyms: TRAMP, 1810049E02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72265
Homologene: 8621
Ahi1
Name: Abelson helper integration site 1
Synonyms: D10Bwg0629e, Jouberin, 1700015F03Rik, Ahi-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52906
VEGA: 10
Homologene: 9762
Plpp1
Name: phospholipid phosphatase 1
Synonyms: Hpic53, Lipid phosphate phosphatase 1, Ppap2a, mPAP, LPP1, LPP-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19012
VEGA: 13
HGNC: HGNC:9228
Homologene: 134049
Ganab
Name: alpha glucosidase 2 alpha neutral subunit
Synonyms: G2an, GluII
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14376
VEGA: 19
HGNC: HGNC:4138
Homologene: 5426
Lypd8
Name: LY6/PLAUR domain containing 8
Synonyms: 2210415F13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70163
Homologene: 52815
Jaml
Name: junction adhesion molecule like
Synonyms: Amica1, LOC270152
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270152
Homologene: 17708
Muc19
Name: mucin 19
Synonyms: apomucin, sld
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239611
Muc5ac
Name: mucin 5, subtypes A and C, tracheobronchial/gastric
Synonyms: MGM, 2210005L13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17833
HGNC: HGNC:7515
Homologene: 137237
Zfp354a
Name: zinc finger protein 354A
Synonyms: Tcf17, kid1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21408
Homologene: 55921
Itpr2
Name: inositol 1,4,5-triphosphate receptor 2
Synonyms: Ip3r2, Itpr5
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16439
HGNC: HGNC:6181
Homologene: 37593
Tnr
Name: tenascin R
Synonyms: TN-R, J1-tenascin, restrictin, janusin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21960
Homologene: 124416
Nlrp9a
Name: NLR family, pyrin domain containing 9A
Synonyms: Nalp-theta, D7Ertd565e, Nalp9a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233001
Homologene: 116072
Brd10
Name: bromodomain containing 10
Synonyms: Gm9832, 9930021J03Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240613
Homologene: 19046
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Ephb6
Name: Eph receptor B6
Synonyms: Mep, Cekl
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13848
HGNC: HGNC:3396
Homologene: 20940
Pcdh10
Name: protocadherin 10
Synonyms: 6430521D13Rik, OL-pc, 6430703F07Rik, Olpc
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18526
Homologene: 74967
Nlrc5
Name: NLR family, CARD domain containing 5
Synonyms: AI451557
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 434341
Homologene: 88935
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239420
Homologene: 65982
Otoa
Name: otoancorin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 246190
Homologene: 71803
Capn8
Name: calpain 8
Synonyms: nCL-2', nCL-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 170725
HGNC: HGNC:1485
Homologene: 15643
Ugt2b38
Name: UDP glucuronosyltransferase 2 family, polypeptide B38
Synonyms: 9430041C03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100559
Homologene: 137225
Masp1
Name: MBL associated serine protease 1
Synonyms: Crarf
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17174
VEGA: 16
HGNC: HGNC:6901
Homologene: 88793
Pdzrn3
Name: PDZ domain containing RING finger 3
Synonyms: Semcap3, LNX3, 1110020C07Rik, semaphorin cytoplasmic domain-associated protein 3A
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 55983
Homologene: 10328
Ubtfl1
Name: upstream binding transcription factor, RNA polymerase I-like 1
Synonyms: B020006M18Rik, Hmgpi
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 546118
Homologene: 110115
Axl
Name: AXL receptor tyrosine kinase
Synonyms: Ufo, Ark, Tyro7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26362
HGNC: HGNC:905
Homologene: 7583
Gcc2
Name: GRIP and coiled-coil domain containing 2
Synonyms: 2600014C01Rik, 0610043A03Rik, 2210420P05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70297
Homologene: 45639
Gm7008
Name: predicted gene 7008
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 629820
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: NaN, NSS2, SNS2, NaT
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24046
VEGA: 9
Homologene: 8041
Nlrp5
Name: NLR family, pyrin domain containing 5
Synonyms: Op1, Mater, Nalp5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23968
Homologene: 65105
Kbtbd12
Name: kelch repeat and BTB (POZ) domain containing 12
Synonyms: 4833415F11Rik, 4933428M03Rik, Klhdc6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 74589
Homologene: 52613
Fmn1
Name: formin 1
Synonyms: Fmn, formin-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14260
HGNC: HGNC:3768
Homologene: 121778
Rhag
Name: Rhesus blood group-associated A glycoprotein
Synonyms: CD241, Rh50A, Rh50
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19743
Homologene: 68045
Or8k24
Name: olfactory receptor family 8 subfamily K member 24
Synonyms: Olfr1058, MOR190-2, GA_x6K02T2Q125-47855818-47854868
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258386
Homologene: 74093
Slc22a2
Name: solute carrier family 22 (organic cation transporter), member 2
Synonyms: Orct2, Oct2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20518
VEGA: 17
Homologene: 68293
Rftn1
Name: raftlin lipid raft linker 1
Synonyms: 2310015N21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76438
Homologene: 18745
Dtx3l
Name: deltex 3-like, E3 ubiquitin ligase
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 209200
Homologene: 51375
Sgms2
Name: sphingomyelin synthase 2
Synonyms: 4933405A16Rik, 5133401H06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74442
Homologene: 32705
Stx19
Name: syntaxin 19
Synonyms: A030009B12Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 68159
Homologene: 55777
Gatd3a
Name: glutamine amidotransferase like class 1 domain containing 3A
Synonyms: D10Jhu81e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 28295
VEGA: 10
HGNC: HGNC:1273
Homologene: 3416
Atp10d
Name: ATPase, class V, type 10D
Synonyms: 9830145H18Rik, D5Buc24e, IMAGE:1069176
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231287
Gm10499
Name: predicted gene 10499
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 69717
Homologene: 128352
Gm13054
Name: predicted gene 13054
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Gm11505
Name: predicted gene 11505
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 13,578,125 bp
  • A to G, chromosome 1 at 159,923,315 bp
  • A to G, chromosome 1 at 161,834,705 bp
  • A to C, chromosome 1 at 182,596,989 bp
  • A to T, chromosome 1 at 193,355,034 bp
  • G to A, chromosome 2 at 41,110,840 bp
  • A to G, chromosome 2 at 86,385,849 bp
  • G to T, chromosome 2 at 102,113,609 bp
  • A to G, chromosome 2 at 113,365,125 bp
  • A to G, chromosome 2 at 119,923,453 bp
  • A to G, chromosome 3 at 45,392,814 bp
  • G to A, chromosome 3 at 88,297,225 bp
  • T to A, chromosome 3 at 131,342,117 bp
  • A to T, chromosome 4 at 48,279,435 bp
  • T to C, chromosome 4 at 148,001,316 bp
  • C to T, chromosome 5 at 72,269,326 bp
  • A to G, chromosome 5 at 87,423,742 bp
  • C to T, chromosome 6 at 41,614,261 bp
  • T to A, chromosome 6 at 88,617,699 bp
  • C to T, chromosome 6 at 101,153,311 bp
  • T to G, chromosome 6 at 116,209,004 bp
  • T to A, chromosome 6 at 146,166,651 bp
  • T to A, chromosome 7 at 23,417,976 bp
  • G to A, chromosome 7 at 25,745,175 bp
  • G to A, chromosome 7 at 25,760,807 bp
  • T to C, chromosome 7 at 25,786,944 bp
  • A to G, chromosome 7 at 26,557,292 bp
  • T to C, chromosome 7 at 49,551,725 bp
  • A to G, chromosome 7 at 121,139,793 bp
  • T to A, chromosome 7 at 141,793,971 bp
  • G to T, chromosome 8 at 94,494,316 bp
  • G to T, chromosome 9 at 18,410,030 bp
  • T to A, chromosome 9 at 45,104,266 bp
  • T to A, chromosome 9 at 119,754,792 bp
  • A to G, chromosome 10 at 20,987,022 bp
  • T to A, chromosome 10 at 58,286,160 bp
  • A to G, chromosome 10 at 78,163,533 bp
  • CGTGACCTTTCTGGT to CGT, chromosome 10 at 85,645,522 bp
  • A to G, chromosome 10 at 108,261,025 bp
  • T to A, chromosome 11 at 51,070,049 bp
  • T to A, chromosome 11 at 58,386,808 bp
  • T to C, chromosome 11 at 87,796,457 bp
  • T to C, chromosome 12 at 40,223,343 bp
  • T to A, chromosome 12 at 78,590,587 bp
  • A to G, chromosome 13 at 108,384,820 bp
  • T to A, chromosome 13 at 112,851,512 bp
  • T to C, chromosome 15 at 47,888,684 bp
  • A to T, chromosome 15 at 91,928,025 bp
  • G to T, chromosome 16 at 23,494,695 bp
  • A to G, chromosome 16 at 35,938,793 bp
  • T to G, chromosome 16 at 62,822,574 bp
  • A to G, chromosome 17 at 12,586,832 bp
  • G to A, chromosome 17 at 36,143,339 bp
  • T to C, chromosome 17 at 40,828,504 bp
  • A to G, chromosome 17 at 50,107,219 bp
  • T to C, chromosome 18 at 12,933,696 bp
  • T to A, chromosome 19 at 8,910,591 bp
  • A to T, chromosome 19 at 29,719,050 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5224 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042797-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.